国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2011年
10期
684-687
,共4页
生长抑素%膝骨性关节炎%自由基代谢
生長抑素%膝骨性關節炎%自由基代謝
생장억소%슬골성관절염%자유기대사
Somatostain%Knee osteoarthritis%Free radical metabolism
目的 探讨生长抑素对家兔膝关节骨性关节炎动物模型自由基代谢的影响,从而探讨生长抑素关节腔内注射治疗骨性关节炎的可能作用机制.方法 取日本大耳白兔40只,随机分成正常对照组、空白模型组、0.9％的氯化钠溶液组、生长抑素组.造模后向生长抑素组右侧膝关节腔内注射生长抑素溶液；向0.9％的氯化钠溶液组右侧膝关节腔内注射0.9％的氯化钠溶液.在造模后10周测量家兔右后膝关节活动度,测定血清一氧化氮和透明质酸的含量,测定滑膜组织中超氧化物歧化酶活性及丙二醛含量.结果 生长抑素组治疗后的兔膝关节活动度为(96.01±1.06)度,较治疗前(50.21±1.80)度明显改善,空白模型治疗后的兔膝关节活动度为(51.89±1.89)度,治疗前为(50.76±1.44)度,改善不明显,两组间差异具有统计学意义(P =0.0025,P＜0.01)；生长抑素组血清一氧化氮含量为(80.14±1.67)μmol/mL,HA含量为(133.73±2.75) μmol/mL,空白模型组血清一氧化氮含量为(111.60±2.76) μmol/mL,HA含量为(309.11±1.89) μmol/mL,两组组间差异有显著统计学意义(P1 =0.0049,P2 =0.0052,P＜0.01)；生长抑素组滑膜组织超氧化物歧化酶活性(24.74±1.67),与空白模型组(15.77±2.76)相比明显升高,差异有显著统计学意义(P =0.0046,P＜0.01),丙二醛含量(0.89±1.46),与空白模型组(1.33±1.03)相比明显降低,且差异有显著统计学意义(P =0.0039,P＜0.01).结论 关节腔内注射生长抑素可以改善家兔膝关节骨性关节炎的氧化应激损伤,提高膝关节的活动度.
目的 探討生長抑素對傢兔膝關節骨性關節炎動物模型自由基代謝的影響,從而探討生長抑素關節腔內註射治療骨性關節炎的可能作用機製.方法 取日本大耳白兔40隻,隨機分成正常對照組、空白模型組、0.9％的氯化鈉溶液組、生長抑素組.造模後嚮生長抑素組右側膝關節腔內註射生長抑素溶液；嚮0.9％的氯化鈉溶液組右側膝關節腔內註射0.9％的氯化鈉溶液.在造模後10週測量傢兔右後膝關節活動度,測定血清一氧化氮和透明質痠的含量,測定滑膜組織中超氧化物歧化酶活性及丙二醛含量.結果 生長抑素組治療後的兔膝關節活動度為(96.01±1.06)度,較治療前(50.21±1.80)度明顯改善,空白模型治療後的兔膝關節活動度為(51.89±1.89)度,治療前為(50.76±1.44)度,改善不明顯,兩組間差異具有統計學意義(P =0.0025,P＜0.01)；生長抑素組血清一氧化氮含量為(80.14±1.67)μmol/mL,HA含量為(133.73±2.75) μmol/mL,空白模型組血清一氧化氮含量為(111.60±2.76) μmol/mL,HA含量為(309.11±1.89) μmol/mL,兩組組間差異有顯著統計學意義(P1 =0.0049,P2 =0.0052,P＜0.01)；生長抑素組滑膜組織超氧化物歧化酶活性(24.74±1.67),與空白模型組(15.77±2.76)相比明顯升高,差異有顯著統計學意義(P =0.0046,P＜0.01),丙二醛含量(0.89±1.46),與空白模型組(1.33±1.03)相比明顯降低,且差異有顯著統計學意義(P =0.0039,P＜0.01).結論 關節腔內註射生長抑素可以改善傢兔膝關節骨性關節炎的氧化應激損傷,提高膝關節的活動度.
목적 탐토생장억소대가토슬관절골성관절염동물모형자유기대사적영향,종이탐토생장억소관절강내주사치료골성관절염적가능작용궤제.방법 취일본대이백토40지,수궤분성정상대조조、공백모형조、0.9％적록화납용액조、생장억소조.조모후향생장억소조우측슬관절강내주사생장억소용액；향0.9％적록화납용액조우측슬관절강내주사0.9％적록화납용액.재조모후10주측량가토우후슬관절활동도,측정혈청일양화담화투명질산적함량,측정활막조직중초양화물기화매활성급병이철함량.결과 생장억소조치료후적토슬관절활동도위(96.01±1.06)도,교치료전(50.21±1.80)도명현개선,공백모형치료후적토슬관절활동도위(51.89±1.89)도,치료전위(50.76±1.44)도,개선불명현,량조간차이구유통계학의의(P =0.0025,P＜0.01)；생장억소조혈청일양화담함량위(80.14±1.67)μmol/mL,HA함량위(133.73±2.75) μmol/mL,공백모형조혈청일양화담함량위(111.60±2.76) μmol/mL,HA함량위(309.11±1.89) μmol/mL,량조조간차이유현저통계학의의(P1 =0.0049,P2 =0.0052,P＜0.01)；생장억소조활막조직초양화물기화매활성(24.74±1.67),여공백모형조(15.77±2.76)상비명현승고,차이유현저통계학의의(P =0.0046,P＜0.01),병이철함량(0.89±1.46),여공백모형조(1.33±1.03)상비명현강저,차차이유현저통계학의의(P =0.0039,P＜0.01).결론 관절강내주사생장억소가이개선가토슬관절골성관절염적양화응격손상,제고슬관절적활동도.
Objective To discuss the effect of somatostain SS on metabolism of free radicals in animal models of osteoarthritis,so to discuss the treatment mechanism of intra- articular injection of somatostatin on osteoarthritis.Methods Forty experimental Japanese big-ear rabbits were randomly divided into four groups:normal control group,blank model group,normal saline group and SS group,n =10 for each group.After modeling,the rats in SS group and normal saline group received intra- articular injection of somatostain and saline respectively on the right knee.Ten weeks after the modeling,we measured the movement of right knee joint in rabbits,the levels of nitric oxide ( NO ) and hyaluronic acid (HA) in serum from the heart blood,and the activity of SOD and the content of MDA in synovial tissue.Results Compared with the blank model group,the movement of the knee joint was significantly increased in SS group,(96.01 ± 1.06)vs (50.21 ± 1.80) (P ＜ 0.01 ).In the blank model group,the levels of NO and HA (μmoL/mL) in serum were ( 111.60 ± 2.76) and ( 309.11 ± 1.89 ),which was significantly decreased in SS group,which was( 80.14 ± 1.67 ) and ( 133.73 ± 2.75 ) ( P1 =0.0049,P2 =0.0052,P ＜ 0.01 ).Compared with the blank model group,the SOD activity was (15.77 ± 2.76) and the MDA in serum nmol/mg prot was( 1.33 ±1.03),while was significantly increased in SS group,(24.74 ± 1.67) ( P ＜ 0.01 ),and the levels of MDA in serum was significantly decreased in the SS group,(0.89 ±1.46) (P＜0.01).Conclusion Intra-articular injection of somatostatin in knee osteoarthritis can improve the oxidative stress,enhance the activity of the knee joint.
