中国医药
中國醫藥
중국의약
CHINA MEDICINE
2010年
5期
426-428
,共3页
结直肠肿瘤%蛋白激酶C%半胱氨酸天冬氨酸蛋白酶3%细胞凋亡
結直腸腫瘤%蛋白激酶C%半胱氨痠天鼕氨痠蛋白酶3%細胞凋亡
결직장종류%단백격매C%반광안산천동안산단백매3%세포조망
Colorectal carcinoma%Protein kinase C-α%Caspase-3%Apoptosis
目的 探讨蛋白激酶Cα(PKC-α)与半胱氨酸天冬氨酸蛋白酶3(Caspase-3)在大肠癌中的表达及其与细胞凋亡的关系.方法 采用免疫组化SP法检测83例大肠组织(正常组织15例,腺瘤组织21例,癌组织47例)中PKC-α和Caspase-3的表达水平,同时采用DNA原位末端标记法标记大肠癌组织中的凋亡细胞.结果 PKC-α在大肠癌组织中阳性表达[31/47例(66.0%)]与正常组织[3/15例(20.0%)]及腺瘤[10/21例(47.6%)]相比,差异有统计学意义(P＜0.05).Caspase-3在正常组织中的阳性表达[12/15例(80.0%)],与腺瘤[13/21例(61.9%)]及大肠癌组织[15/47例(31.9%)]相比,差异有统计学意义(P＜0.05),Caspase-3的阳性表达仅与大肠癌组织分化程度关系密切(P＜0.05).PKC-α阳性表达的大肠癌组织中细胞凋亡指数(1.62±0.57)明显低于阴性组(3.28±0.75)(P＜0.05),Caspase-3阳性组细胞凋亡指数(2.40±0.55)明显高于阴性组(1.25±0.59)(P＜0.05).PKC-α与Caspase-3的表达呈负相关(r=-0.498,P＜0.05).结论 PKC-α和Caspase-3与大肠癌的细胞凋亡密切相关,参与细胞凋亡的调节机制,在大肠癌恶变的过程中发挥重要作用.
目的 探討蛋白激酶Cα(PKC-α)與半胱氨痠天鼕氨痠蛋白酶3(Caspase-3)在大腸癌中的錶達及其與細胞凋亡的關繫.方法 採用免疫組化SP法檢測83例大腸組織(正常組織15例,腺瘤組織21例,癌組織47例)中PKC-α和Caspase-3的錶達水平,同時採用DNA原位末耑標記法標記大腸癌組織中的凋亡細胞.結果 PKC-α在大腸癌組織中暘性錶達[31/47例(66.0%)]與正常組織[3/15例(20.0%)]及腺瘤[10/21例(47.6%)]相比,差異有統計學意義(P＜0.05).Caspase-3在正常組織中的暘性錶達[12/15例(80.0%)],與腺瘤[13/21例(61.9%)]及大腸癌組織[15/47例(31.9%)]相比,差異有統計學意義(P＜0.05),Caspase-3的暘性錶達僅與大腸癌組織分化程度關繫密切(P＜0.05).PKC-α暘性錶達的大腸癌組織中細胞凋亡指數(1.62±0.57)明顯低于陰性組(3.28±0.75)(P＜0.05),Caspase-3暘性組細胞凋亡指數(2.40±0.55)明顯高于陰性組(1.25±0.59)(P＜0.05).PKC-α與Caspase-3的錶達呈負相關(r=-0.498,P＜0.05).結論 PKC-α和Caspase-3與大腸癌的細胞凋亡密切相關,參與細胞凋亡的調節機製,在大腸癌噁變的過程中髮揮重要作用.
목적 탐토단백격매Cα(PKC-α)여반광안산천동안산단백매3(Caspase-3)재대장암중적표체급기여세포조망적관계.방법 채용면역조화SP법검측83례대장조직(정상조직15례,선류조직21례,암조직47례)중PKC-α화Caspase-3적표체수평,동시채용DNA원위말단표기법표기대장암조직중적조망세포.결과 PKC-α재대장암조직중양성표체[31/47례(66.0%)]여정상조직[3/15례(20.0%)]급선류[10/21례(47.6%)]상비,차이유통계학의의(P＜0.05).Caspase-3재정상조직중적양성표체[12/15례(80.0%)],여선류[13/21례(61.9%)]급대장암조직[15/47례(31.9%)]상비,차이유통계학의의(P＜0.05),Caspase-3적양성표체부여대장암조직분화정도관계밀절(P＜0.05).PKC-α양성표체적대장암조직중세포조망지수(1.62±0.57)명현저우음성조(3.28±0.75)(P＜0.05),Caspase-3양성조세포조망지수(2.40±0.55)명현고우음성조(1.25±0.59)(P＜0.05).PKC-α여Caspase-3적표체정부상관(r=-0.498,P＜0.05).결론 PKC-α화Caspase-3여대장암적세포조망밀절상관,삼여세포조망적조절궤제,재대장암악변적과정중발휘중요작용.
Objective To study the expression of protein kinase C (PKC)-α and Caspase-3 in colorectal carcinoma and to analyze their relation with apoptosis.Methods Immunohistochemistry and the TdT-mediated dUTP nick and labeling(TUNEL) were used to investigate apoptosis and the alteration of PKC-α and Caspase-3 in 47 cases of colorectal carcinomas,21 colorectal adenomas and 15 normal tissues.Results The positive rates of PKC-α expression in normal colorectal tissues,adenomas and carcinomas were 20.0%,47.6%,66.0%,respectively(P＜0.05).Overexpression of Caspase-3 was 31.9 %,61.9%,80.0% in carcinomas,adenomas and normal colorectal tissues,respectively (P＜0.05),which was correlated with turnout cellular differentiation (P＜0.05).The apoptosis index (AI) of was significantly lower than that of PKC-α-negative cases (1.62±0.57 vs 3.28±0.75) (P＜0.05).The apoptosis index of Caspase-3-positive cases was significantly higher than that of Caspase-3-negative cases (2.40±0.55 vs 1.25±0.59) (P＜0.05).The expression of PKC-α was negatively correlated with Caspase-3 (r=-0.498,P＜0.05).Conclusions The expression of PKC-α and Caspase-3 is closely associated with apoptosis in colorectal carcinoma.The PKC-α and Caspase-3 may participate in the mechanism of apoptosis and play important roles in malignance of colorectal carcinoma.
