中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
2期
218-222
,共5页
张亚楠%崔炜%韩梅%郑斌%刘凡%谢瑞芹%杨晓红%谷国强%郑红梅%温进坤
張亞楠%崔煒%韓梅%鄭斌%劉凡%謝瑞芹%楊曉紅%穀國彊%鄭紅梅%溫進坤
장아남%최위%한매%정빈%류범%사서근%양효홍%곡국강%정홍매%온진곤
华法林%基因多态性%多聚酶链反应%限制性片段长度多态性
華法林%基因多態性%多聚酶鏈反應%限製性片段長度多態性
화법림%기인다태성%다취매련반응%한제성편단장도다태성
Warfarin%Gene polymorphism%Polymerase chain reaction%Restriction fragment length polymorphism
目的 了解河北省汉族人群CYP450 2C9和VKORC1的基因多态性分布与其他不同民族之间的差异,探讨CYP450 2C9~*3+1075C/A和VKORC1-1639A/G基因多态性与华法林剂量的关系.方法 随机选取门诊体检的健康人487名,检测CYP450 2C9~*3+1075C/A和VKORC1-1639A/G基因型及其分布频率,并与国外多个民族的CYP450 2C9~*3+1075C/A和VKORC1-1639A/G的基因多态性进行比较.选取服用华法林稳定剂量的患者101例,检测其基闪型并结合其个体差异和华法林的稳定剂量进行分析.结果 487名正常人CYP450 2C9*3+1075C/A基因型中,AA型为449名(92.2%),AC型36名(7.4%),CC型2名(0.4%);VKORC1-1639A/G基因型中AA型为415名(85.2%),GA型72名(14.8%),未发现GG型(0.0%).采用多元逐步回归得出有关华法林稳定剂量的方程:In(D)=0.346+0.017(体重)-0.376(CYP450 2C9~*3+1075C/A)+0.148(VKORC1-1639A/G)-0.002(年龄)(r=0.827,P=0.02).结论 汉族人群CYP450 2C9和VKORC1存在明显的基因多态性.CYP450 2C9~*3+1075C/A和VKORC1-1639A/G的基因型和患者个体之间的体重、年龄差异是影响华法林稳定剂量的因素.
目的 瞭解河北省漢族人群CYP450 2C9和VKORC1的基因多態性分佈與其他不同民族之間的差異,探討CYP450 2C9~*3+1075C/A和VKORC1-1639A/G基因多態性與華法林劑量的關繫.方法 隨機選取門診體檢的健康人487名,檢測CYP450 2C9~*3+1075C/A和VKORC1-1639A/G基因型及其分佈頻率,併與國外多箇民族的CYP450 2C9~*3+1075C/A和VKORC1-1639A/G的基因多態性進行比較.選取服用華法林穩定劑量的患者101例,檢測其基閃型併結閤其箇體差異和華法林的穩定劑量進行分析.結果 487名正常人CYP450 2C9*3+1075C/A基因型中,AA型為449名(92.2%),AC型36名(7.4%),CC型2名(0.4%);VKORC1-1639A/G基因型中AA型為415名(85.2%),GA型72名(14.8%),未髮現GG型(0.0%).採用多元逐步迴歸得齣有關華法林穩定劑量的方程:In(D)=0.346+0.017(體重)-0.376(CYP450 2C9~*3+1075C/A)+0.148(VKORC1-1639A/G)-0.002(年齡)(r=0.827,P=0.02).結論 漢族人群CYP450 2C9和VKORC1存在明顯的基因多態性.CYP450 2C9~*3+1075C/A和VKORC1-1639A/G的基因型和患者箇體之間的體重、年齡差異是影響華法林穩定劑量的因素.
목적 료해하북성한족인군CYP450 2C9화VKORC1적기인다태성분포여기타불동민족지간적차이,탐토CYP450 2C9~*3+1075C/A화VKORC1-1639A/G기인다태성여화법림제량적관계.방법 수궤선취문진체검적건강인487명,검측CYP450 2C9~*3+1075C/A화VKORC1-1639A/G기인형급기분포빈솔,병여국외다개민족적CYP450 2C9~*3+1075C/A화VKORC1-1639A/G적기인다태성진행비교.선취복용화법림은정제량적환자101례,검측기기섬형병결합기개체차이화화법림적은정제량진행분석.결과 487명정상인CYP450 2C9*3+1075C/A기인형중,AA형위449명(92.2%),AC형36명(7.4%),CC형2명(0.4%);VKORC1-1639A/G기인형중AA형위415명(85.2%),GA형72명(14.8%),미발현GG형(0.0%).채용다원축보회귀득출유관화법림은정제량적방정:In(D)=0.346+0.017(체중)-0.376(CYP450 2C9~*3+1075C/A)+0.148(VKORC1-1639A/G)-0.002(년령)(r=0.827,P=0.02).결론 한족인군CYP450 2C9화VKORC1존재명현적기인다태성.CYP450 2C9~*3+1075C/A화VKORC1-1639A/G적기인형화환자개체지간적체중、년령차이시영향화법림은정제량적인소.
Objective To investigate the distribution of gene polymorphism of CYP450 2C9 and VKORC1-1639A/G in the Chinese population as well as the difference of genetic polymorphism between Chinese Han population and other ethnic populations.Contribution of CYP2C9 and VKORC1 genotype to the maintenance doses on warfarin was also studied.Methods The genotype and allele frequencies were calculated and compared with those in other populations.One hundred and one patients with stable anticoagulation with warfarin under a target international normalized ratio(INR)of 2.0 to 3.0 were enrolled for studying the relationship between the CYP2C9 and VKORC1 gene polymorphism and the warfarin maintaining dosage.Results CYP450 2C9~*3 + 1075C/A allele frequencies were:AA in 449 cases(92.2%),AC in 36 cases(7.4%)and CC in 2 cases(0.4%),respectively.VKORC1-1639A/G allele frequencies were AA in 415 cases(85.2%),GA in 72 cases(14.8%),but GG in no case(0.0%),respectively.When linear stepwise regression analysis was used to identify factors contributing to warfarin stable dose,the final equation was:ln(D)=0.346 + 0.017(weight)-0.376(CYP450 2C9~*3 + 1075C/A)+ 0.148(VKORC1-1639A/G)-0.002(age)(r=0.827,P=0.02).Conclusion There existed significant gene polymorphism CYP450 2C9~*3 + 1075C/A and VKORC1-1639A/G in the Chinese Han population.Both Gene polymorphisms of CYP450 2C9*3 + 1075C/A and VKORC1-1639A/G were significantly affecting the maintaining dose of warfarin in the Chinese population.
