CAJ | 학술논문

目的探讨异源动物蓝舌病毒dsRNA诱导人源细胞产生干扰素(IFN)的能力.方法借助体外培养系统,利用ELISA技术检测IFN诱导剂多聚次黄苷酸-胞苷酸(PolyI:C)、蓝舌病毒(BTV)及BTV dsRNA 3种不同处理因素作用后,培养细胞上清液中IFN-β的含量,以比较研究其诱导人肺癌细胞A549和人胚肺细胞HEL产生IFN-β的能力和特征.结果这3种因子均能诱导人肺癌A549细胞和人正常肺细胞HEL产生IFN-β,BTV dsRNA诱导IFN-β产生的能力显著高于Poly I:C和BTV.浓度高的BTV dsRNA诱导细胞产生的IFN-β水平高,说明二者之间存在剂量依赖性.BTVdsRNA对不同性质的人源细胞(人肺癌A549细胞、人正常肺细胞HEL)具有不同的诱导产生IFN-β的能力,诱导人正常肺细胞HEL产生IFN-β的能力显著地高于诱导人肺癌细胞A549产生IFN-β的能力(P<0.05).结论裸露的BTV dsRNA分子能有效地诱导人源细胞产生IFN-β;结合BTV和dsRNA对人的安全性以及人类正在努力寻找新的内源性IFN诱导剂的事实,可以推断BTV dsRNA具有发展成内源性IFN诱导剂之潜能.
목적 탐토이원동물람설병독dsRNA유도인원세포산생간우소(IFN)적능력.방법 차조체외배양계통,이용ELISA기술검측IFN유도제다취차황감산-포감산(PolyI:C)、람설병독(BTV)급BTV dsRNA 3충불동처리인소작용후,배양세포상청액중IFN-β적함량,이비교연구기유도인폐암세포A549화인배폐세포HEL산생IFN-β적능력화특정.결과 저3충인자균능유도인폐암A549세포화인정상폐세포HEL산생IFN-β,BTV dsRNA유도IFN-β산생적능력현저고우Poly I:C화BTV.농도고적BTV dsRNA유도세포산생적IFN-β수평고,설명이자지간존재제량의뢰성.BTVdsRNA대불동성질적인원세포(인폐암A549세포、인정상폐세포HEL)구유불동적유도산생IFN-β적능력,유도인정상폐세포HEL산생IFN-β적능력현저지고우유도인폐암세포A549산생IFN-β적능력(P<0.05).결론 라로적BTV dsRNA분자능유효지유도인원세포산생IFN-β;결합BTV화dsRNA대인적안전성이급인류정재노력심조신적내원성IFN유도제적사실,가이추단BTV dsRNA구유발전성내원성IFN유도제지잠능.
Objective To investigate the capability of bluetongue virus(BTV)dsRNA inducing IFN-β from human cells.Methods Artificial complex interfemn inducer polyinosinic-polycytidylic acid (PolyI:C).BTV and BTV dsRNA were added to A549(human lung cancer cell)and HEL(human lung normal cells)culture system in difierent concentrations.IFN-β in culture median was detected by ELISA.Results Though all of the 3 reagents could induce IFN-β,BTV dsRNA significanay induced the highest level of IFN-β.The production of IFN-β was induced by BTV dsRNA in dose dependence.BTV dsRNA induced IFN-β level from HEL Was higher than that from A549(P<0.05).Conclusion BTV dsRNA Can induce IFN-β from human cells effectively,which shows its potential of an endogenous IFN-β inducer.