CAJ | 학술논문

目的以新型可注射生物材料壳聚糖β-磷酸三钙为支架,负载骨髓间充质干细胞(MSCs)与富血小板血浆(PRP)构建成新型可注射组织工程骨,观察其体内成骨效应.方法采用中国青山羊双侧胫骨平台下腔穴型骨缺损模型.30只中国青山羊随机分为五组:空白组:骨缺损部不植入任何组织工程材料;单纯材料组:单纯植入组织工程材料壳聚糖β-磷酸三钙;PRP组:植入单纯复合PRP组织工程材料:MSCs组:植入单纯复合MSCs的组织工程材料;PRP/MSCs组:植入复合PRP、MSCs的组织工程材料.于术后第4、8周取出骨缺损区标本进行大体观察和组织学切片观察,图像分析骨缺损区域骨小梁的生成数量.结果术后8周,大体观察显示PRP/MSC组骨缺损区域表面新生骨连续,外观类似正常骨.术后4、8周,组织学显示PRP/MSCs组骨缺损边缘区域类骨质数量明显增多,骨缺损部多为点片状新生骨组织,其中大的片状新生骨组织明显增多.术后4周空白组、单纯材料组、PRP组、MSCs组、PRP/MSCs组的成骨面积百分比分别为8.79±3.63、14.49±3.72、24.18±5.38、24.42±5.10、31.10±3.49:8周时分别为15.41±4.21、25.36±5.37、30.71±4.39、33.97±4.45、48.60±5.97,4周、8周PRP/MSCs组骨修复效果均优于其他各组(P<0.05).结论负载PRP和MSCs的新型可注射组织工程骨具有良好的骨修复作用.
목적 이신형가주사생물재료각취당β-린산삼개위지가,부재골수간충질간세포(MSCs)여부혈소판혈장(PRP)구건성신형가주사조직공정골,관찰기체내성골효응.방법 채용중국청산양쌍측경골평태하강혈형골결손모형.30지중국청산양수궤분위오조:공백조:골결손부불식입임하조직공정재료;단순재료조:단순식입조직공정재료각취당β-린산삼개;PRP조:식입단순복합PRP조직공정재료:MSCs조:식입단순복합MSCs적조직공정재료;PRP/MSCs조:식입복합PRP、MSCs적조직공정재료.우술후제4、8주취출골결손구표본진행대체관찰화조직학절편관찰,도상분석골결손구역골소량적생성수량.결과 술후8주,대체관찰현시PRP/MSC조골결손구역표면신생골련속,외관유사정상골.술후4、8주,조직학현시PRP/MSCs조골결손변연구역류골질수량명현증다,골결손부다위점편상신생골조직,기중대적편상신생골조직명현증다.술후4주공백조、단순재료조、PRP조、MSCs조、PRP/MSCs조적성골면적백분비분별위8.79±3.63、14.49±3.72、24.18±5.38、24.42±5.10、31.10±3.49:8주시분별위15.41±4.21、25.36±5.37、30.71±4.39、33.97±4.45、48.60±5.97,4주、8주PRP/MSCs조골수복효과균우우기타각조(P<0.05).결론 부재PRP화MSCs적신형가주사조직공정골구유량호적골수복작용.
Objective To study the effect of a novel injectable scaffold material chitosan- beta-TCP combining bone marrow mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) on repairing bone defect of goat. Methods The model of the studies was 12ram diameter circular hole tibia bone defect of goat. 30 Chinese goats were raudomly divided into 5 groups: blank group: nothing was embeded in bone defect; simple material group: the material embeded in bone defect was chitosan-beta-TCP; PRP group: the material was chitesan-beta-TCP combining PRP; MSCs group: the material was chitosan-beta-TCP combining MSCs; PRP/MSCs group:the material was chitosan-beta-TCP combining MSCs and PRP. At 4,8 weeks after operation, the samples were observed, histological and image analysis were used to evaluate the effect of bone regeneration. Results At 8 weeks, the surface of bone defect zone of PRP/MSCs group were coverd by continuous new bones, like normal bone. Histological slice showed the esteoid at boundary of normal bone of MSCs/PRP group obviously increased compare to other groups at the 4th or 8th week after operation respectively. The new bone tissues of bone defect were punctiform or lamellar new bone tissues, in which the proportion of big lamellar new bone tissue obviously increased. Image analysis showed that the areas of balnk group, simple material group, PRP group, MSCs group, PRP/MSCs group were 8.79±3.63,14.49± 3.72,24.18 ± 5.38,24.42 ± 5.10,31.10 ± 3.49 at 4 weeks and 15.41 ± 4.21,25.36 ± 5.37,30.71 ± 4.39, 33.97 ± 4.45,48.60 ± 5.97 at 8 weeks respectively. The effect of bone regeneration of PRP/MSCs group was better than other groups (P < 0.05). Conclusion The injectable tissue-engineering bone constructed with chitosan-beta-TCP, MSCs and PRP possesses good ability on repairing bone defect.