低频电疗对白鼠周围神经损伤后脊髓后角神经细胞活动电位的影响
저빈전료대백서주위신경손상후척수후각신경세포활동전위적영향
Effect of low-frequency transcutaneous electrical nerve stimulation on the action potential of spinal cord posterior horn cells in rats after peripheral nerve injury
  • 万方数据
    中国临床康复 중국림상강복
    CHINESE JOURNAL OF CLINICAL REHABILITATION
    2006年 22期 183-186 ,共4页

    崔松彪%赵和荣%吴光%朴虎男%许梅花

    최송표%조화영%오광%박호남%허매화

    疼痛%周围神经/损伤%电刺激疗法 疼痛%週圍神經/損傷%電刺激療法
    동통%주위신경/손상%전자격요법
    背景:迄今为止有关低频电疗缓解疼痛作用机制的研究寥寥无几,且有关低频电疗对脊髓后角神经细胞活动电位的影响尚不清楚.目的:应用周围神经损伤的动物模型,观察低频电疗对被机械性刺激和温度刺激所引发的脊髓后角神经细胞活动电位(膜电位)的影响,并观察纳洛酮干预后的效应.设计:随机对照动物实验.单位:延边大学医学院附属医院神经内科.材料:实验于2004-02/10在延边大学医学院中心实验室进行.取80只SD雄性白鼠,随机取60只手术分离出坐骨神经,将坐骨神经的2个分支胫神经和腓肠神经结扎后切断,留下腓神经作为实验组,其余20只经手术分离出坐骨神经后放原位,重新缝合皮肤作为对照组.方法:①疼痛测定:手术1周后用机械性刺激和温度刺激每5 s刺激大鼠一次,共刺激10次后测定躲避反应的频率(0%～40%为轻度疼痛,40%～70%为中度,70%以上为重度).②对照组和实验组中重度疼痛的大鼠测定自发的和被机械性刺激和温度刺激所引发的脊髓后角神经细胞膜电位.③实验组大鼠用环状电极在腿部进行经皮低频电刺激(电流3 mA,时间10 min,频率10 Hz),测定刺激前后脊髓后角神经细胞膜电位.④实验组低频电刺激的同时,经尾部静脉注射纳洛酮,测定注射纳洛酮前和注射10 min后脊髓后角神经细胞膜电位.结果:经补充后80只大鼠进入结果分析.①实验组被机械性刺激和温度刺激所引发的躲避反应频率明显高于对照组(P＜0.01).②实验组被机械性刺激和温度刺激所引发的脊髓后角神经细胞膜电位明显高于对照组(P＜0.01).③实验组经低频电刺激10 min后,被机械性刺激和温度刺激所引发的脊髓后角神经细胞的膜电位明显低于刺激前[每10 s(102.6±0.86),(136.9±1.46)次;每10 s(175.2±1.28),(240.8±1.51)次,P＜0.01].④实验组注射纳洛酮10 min后,被机械性刺激和温度刺激所引发的脊髓后角神经细胞的膜电位明显高于注射前[每10 s(174.5±0.41),(235.4±1.41)次,P＜0.01].结论:低频电刺激能有效抑制被无害刺激所引发的脊髓后角神经细胞的活动电位,且静脉注射纳洛酮(8 mg/kg)可使之逆转到治疗前的水平,说明低频电疗可能是刺激中枢神经系统使其分泌内源鸦片物质,作用于脊髓后角细胞使其活性降低,从而达到缓解疼痛的目的.
    배경:흘금위지유관저빈전료완해동통작용궤제적연구요요무궤,차유관저빈전료대척수후각신경세포활동전위적영향상불청초.목적:응용주위신경손상적동물모형,관찰저빈전료대피궤계성자격화온도자격소인발적척수후각신경세포활동전위(막전위)적영향,병관찰납락동간예후적효응.설계:수궤대조동물실험.단위:연변대학의학원부속의원신경내과.재료:실험우2004-02/10재연변대학의학원중심실험실진행.취80지SD웅성백서,수궤취60지수술분리출좌골신경,장좌골신경적2개분지경신경화비장신경결찰후절단,류하비신경작위실험조,기여20지경수술분리출좌골신경후방원위,중신봉합피부작위대조조.방법:①동통측정:수술1주후용궤계성자격화온도자격매5 s자격대서일차,공자격10차후측정타피반응적빈솔(0%～40%위경도동통,40%～70%위중도,70%이상위중도).②대조조화실험조중중도동통적대서측정자발적화피궤계성자격화온도자격소인발적척수후각신경세포막전위.③실험조대서용배상전겁재퇴부진행경피저빈전자격(전류3 mA,시간10 min,빈솔10 Hz),측정자격전후척수후각신경세포막전위.④실험조저빈전자격적동시,경미부정맥주사납락동,측정주사납락동전화주사10 min후척수후각신경세포막전위.결과:경보충후80지대서진입결과분석.①실험조피궤계성자격화온도자격소인발적타피반응빈솔명현고우대조조(P＜0.01).②실험조피궤계성자격화온도자격소인발적척수후각신경세포막전위명현고우대조조(P＜0.01).③실험조경저빈전자격10 min후,피궤계성자격화온도자격소인발적척수후각신경세포적막전위명현저우자격전[매10 s(102.6±0.86),(136.9±1.46)차;매10 s(175.2±1.28),(240.8±1.51)차,P＜0.01].④실험조주사납락동10 min후,피궤계성자격화온도자격소인발적척수후각신경세포적막전위명현고우주사전[매10 s(174.5±0.41),(235.4±1.41)차,P＜0.01].결론:저빈전자격능유효억제피무해자격소인발적척수후각신경세포적활동전위,차정맥주사납락동(8 mg/kg)가사지역전도치료전적수평,설명저빈전료가능시자격중추신경계통사기분비내원아편물질,작용우척수후각세포사기활성강저,종이체도완해동통적목적.
    BACKGROUND: Up to now, few studies related to the mechanism of low-frequency transcutaneous electrical nerve stimulation (TENS) in relieving pain, and the effect of low-frequency TENS on the activity potential of dorsal horn cells in rats after peripheral nerve injury. OBJECTIVE: To observe the effects of low-frequency TENS on the activity potential of dorsal horn cells induced by mechanical allodynia and thermal allodynia by using animal models of peripheral nerve injury, and observe the efficacy after interfering of naloxone. DESIGN: A randomized control animal study. SETTING: Department of Neurology, Affiliated Hospital of Medical College, Yanbian University. MATERIALS: The experiment was carried out in the central laboratory of Medical College, Yanbian University between February and October 2004. Eighty male Sprague-Dawley rats were used, and 60 random selected ones were operated to separate sciatic nerve, two branch tibial nerves and sural nerves of sciatic nerve were amputated after ligation, and peroneal nerve was left as the experimental group; the other 20 rats were placed at the origin after sciatic nerve was separated, and then the skin was sutured as the control group. METHODS: ① Pain detection (Behavioral test): At 1 week postoperatively, the rats were given mechanical allodynia and thermal allodynia once every 5 seconds for 10 times, and then the frequency of foot withdrawal was detected (0%-40% for mild pain, 40%-70% for moderate pain; 70% and above for severe pain). ② The spontaneous activity potential of dorsal horn cells and that induced by mechanical allodynia and thermal allodynia were detected among the rats with moderate and severe pain in the control group and study group. ③ Low-frequency TENS of 3 mA and 10 Hz was applied to the legs of rats in the experimental group with annular electrode for 10 minute, and the membrane potential of dorsal horn cell was detected before and after stimulation. ④ At the same time of low-frequency TENS was given, rats in the experimental group were injected with naloxone via audal vein, and the membrane potential of dorsal horn cell was detected before and at 10 minutes after naloxone injection.RESULTS: Finally 80 rats were involved in the analysis of results after compensation. ① The foot withdrawal frequencies induced by mechanical allodynia and thermal allodynia in the experimental group were obviously higher than those in the control group (P ＜ 0.01). ② The membrane potential of dorsal horn cell by mechanical allodynia and thermal allodynia in the experimental group were obviously higher than those in the control group (P ＜ 0.01). ③ The membrane potential of dorsal horn cell by mechanical allodynia and thermal allodynia at 10 minutes after low-frequency TENS in the experimental group were obviously higher than those in the control group [(102.6±0.9), (136.9±1.46) impulses per 10 seconds; (175.2±1.28), (240.8±1.51) impulses per 10 seconds, P ＜ 0.01]. ④ In the experimental group, the membrane potential of dorsal horn cell by mechanical allodynia and thermal allodynia at 10 minutes after naloxone injection were obviously higher than those before injection [(174.5±0.4), (235.4±1.4) impulses per 10 seconds, P ＜ 0.01].CONCLUSION: Low-frequency TENS can effectively inhibit the activity potential of spinal dorsal horn cells induced by non-noxious stimulation,and the intravenous injection of naloxone (8 mg/kg) can recover it to the pretreatment level, indicating that low-frequency TENS may alleviate pain by stimulating central nervous system to make it secrete endogenous opium system, and acting on spinal dorsal horn cells to reduce the activity.
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