CAJ | 학술논문

目的:探讨雷公藤甲素对外周血单个核细胞(PBMC)分泌自细胞介素-1β(IL-1β)的抑制作用与IL-1β基因多态性的关系.方法:采用PCR-RFLP法对31名健康志愿者IL-1β基因启动子区-511位点C-T多态性进行基因型检测,同时进行PBMC培养,用脂多糖(LPS)刺激培养细胞,并予以雷公藤甲素处理PBMC,收集培养上清液,ELISA法检测上清液中IL-1β的含量.结果:携带IL-1β-511T/T纯合子基因型PBMC经LPS刺激后IL-1β的分泌量明显较非T/T纯合子为高(P＜0.05);雷公藤甲素能显著抑制LPS诱导的C/C和C/T基因型PBMC分泌IL-1β(P＜0.05),但对T/T基因型的抑制作用不明显(P＞0.05).结论:IL-1β基因-511C-T多态性与IL-1β的分泌量相关,雷公藤甲素对不同基因型的IL-1β抑制作用有差异,这可能是导致雷公藤药理作用出现个体差异的原因之一.
목적:탐토뢰공등갑소대외주혈단개핵세포(PBMC)분비자세포개소-1β(IL-1β)적억제작용여IL-1β기인다태성적관계.방법:채용PCR-RFLP법대31명건강지원자IL-1β기인계동자구-511위점C-T다태성진행기인형검측,동시진행PBMC배양,용지다당(LPS)자격배양세포,병여이뢰공등갑소처리PBMC,수집배양상청액,ELISA법검측상청액중IL-1β적함량.결과:휴대IL-1β-511T/T순합자기인형PBMC경LPS자격후IL-1β적분비량명현교비T/T순합자위고(P＜0.05);뢰공등갑소능현저억제LPS유도적C/C화C/T기인형PBMC분비IL-1β(P＜0.05),단대T/T기인형적억제작용불명현(P＞0.05).결론:IL-1β기인-511C-T다태성여IL-1β적분비량상관,뢰공등갑소대불동기인형적IL-1β억제작용유차이,저가능시도치뢰공등약리작용출현개체차이적원인지일.
AIM: To explore whether the inhibitory effect of triptolide on IL - 1β production by PBMC is asso ciated with IL - 1β gene polymorphisms. METHODS: IL - 1β gene polymorphism was analyzed in 31 healthy volunteers. From genomic DNA, the C - T polymorphism at IL - 1 β - 511 was typed by PCR - RFLP. Meanwhile the IL - 1 β was also measured in the supernatants of the cultured and stimulated peripheral blood mononuclear cells (PBMC) by ELISA. RE SULTS: After LPS stimulation in PBMC cultures of healthy subjects, the secretion levels of IL - 1 β in 9 volunteers who carried IL - 1β -511 T/T genotype were higher than in volunteers who are not T/T genotype (P ＜0.05). Triptolide sup pressed the production of IL - 1β significantly in LPS - treated human PBMC carried C/C and C/T genotype ( P ＜ 0.05 ), but this significant inhibitory effect of triptolide was not seen in T/T genotype ( P ＞ 0.05 ). CONCLUSION: The gene polymorphism at IL - 1β - 511 was related to the production of IL - 1β, and the inhibitory effect of triptolide on the produc tion of IL - 1β was different in C/C, C/T, T/T genotype of IL - 1β -511, which may be one of the reasons for the phe nomenon that people respond differently to triptolide.