湖南师范大学学报（医学版）
湖南師範大學學報（醫學版）
호남사범대학학보（의학판）
JOURNAL OF HUNAN NORMAL UNIVERSITY(MEDICAL SCIENCE)
2007年
3期
1-5
,共5页
Elifuraha Francis Mchomvu%杨作成%孙跃女%李小明%陈淳媛
Elifuraha Francis Mchomvu%楊作成%孫躍女%李小明%陳淳媛
Elifuraha Francis Mchomvu%양작성%손약녀%리소명%진순원
Ⅰ型胶原%Ⅳ型胶原%转移生长因子β1%成纤维细胞,心脏%大鼠
Ⅰ型膠原%Ⅳ型膠原%轉移生長因子β1%成纖維細胞,心髒%大鼠
Ⅰ형효원%Ⅳ형효원%전이생장인자β1%성섬유세포,심장%대서
Collagen Ⅰ%Collagen Ⅳ%TGF-β1%Fibroblast,cardiac%rat
目的:观察TGF-β1对原代培养的新生大鼠心肌成型与Ⅳ胶原表达的影响.方法:取出生3～7d的SD大鼠心脏,利用酶消化法结合差速贴壁体外培养心肌成纤维细胞;分别用不同浓度的TGF-β1(TGF-β1=10 ng/mL 、1ng/mL、0 ng/mL)干预心肌成纤维细胞,分别于干预后5h、24h,采用RT-PCR检测I 型胶原和Ⅳ型胶原的表达.结果:1.用0ng/mL(对照组), 1ng/mL和10ng/mL的TGF-β1干预心肌成纤维细胞5h, RT-PCR检测I 型胶原/β-actin光密度值分别为0.61±0.02, 0.73±0.03 和 0.86±0.04,光密度值随着TGF-β1浓度的增加而增加,各组比较,差异有显著性(P＜0.01);干预心肌成纤维细胞24h后,RT-PCR检测I 型胶原/β-actin光密度值分别为0.65±.03, 0.91±0.02 和 0.98±0.02,光密度值随着TGF-β1浓度的增加而增加,各组比较,差异有显著性(P＜0.01).2.用0 ng/mL(对照组), 1ng/mL and 10ng/mL的TGF-β1干预心肌成纤维细胞5h, RT-PCR检测Ⅳ型胶原/β-actin光密度值分别为0.58±0.06, 0.71±0.02, 0.87±0.02,光密度值随着TGF-β1浓度的增加而增加,各组比较,差异有显著性(P＜0.01);干预心肌成纤维细胞24h后,RT-PCR检测Ⅳ型胶原/β-actin光密度值分别为0.62±0.01, 0.83±0.05, 0.96±0.02,光密度值随着TGFβ1浓度的增加而增加,各组比较,差异有显著性(P＜0.01).结论:TGFβ1能加强I型和Ⅳ型胶原的表达,在24小时内呈时间和剂量依赖性.
目的:觀察TGF-β1對原代培養的新生大鼠心肌成型與Ⅳ膠原錶達的影響.方法:取齣生3～7d的SD大鼠心髒,利用酶消化法結閤差速貼壁體外培養心肌成纖維細胞;分彆用不同濃度的TGF-β1(TGF-β1=10 ng/mL 、1ng/mL、0 ng/mL)榦預心肌成纖維細胞,分彆于榦預後5h、24h,採用RT-PCR檢測I 型膠原和Ⅳ型膠原的錶達.結果:1.用0ng/mL(對照組), 1ng/mL和10ng/mL的TGF-β1榦預心肌成纖維細胞5h, RT-PCR檢測I 型膠原/β-actin光密度值分彆為0.61±0.02, 0.73±0.03 和 0.86±0.04,光密度值隨著TGF-β1濃度的增加而增加,各組比較,差異有顯著性(P＜0.01);榦預心肌成纖維細胞24h後,RT-PCR檢測I 型膠原/β-actin光密度值分彆為0.65±.03, 0.91±0.02 和 0.98±0.02,光密度值隨著TGF-β1濃度的增加而增加,各組比較,差異有顯著性(P＜0.01).2.用0 ng/mL(對照組), 1ng/mL and 10ng/mL的TGF-β1榦預心肌成纖維細胞5h, RT-PCR檢測Ⅳ型膠原/β-actin光密度值分彆為0.58±0.06, 0.71±0.02, 0.87±0.02,光密度值隨著TGF-β1濃度的增加而增加,各組比較,差異有顯著性(P＜0.01);榦預心肌成纖維細胞24h後,RT-PCR檢測Ⅳ型膠原/β-actin光密度值分彆為0.62±0.01, 0.83±0.05, 0.96±0.02,光密度值隨著TGFβ1濃度的增加而增加,各組比較,差異有顯著性(P＜0.01).結論:TGFβ1能加彊I型和Ⅳ型膠原的錶達,在24小時內呈時間和劑量依賴性.
목적:관찰TGF-β1대원대배양적신생대서심기성형여Ⅳ효원표체적영향.방법:취출생3～7d적SD대서심장,이용매소화법결합차속첩벽체외배양심기성섬유세포;분별용불동농도적TGF-β1(TGF-β1=10 ng/mL 、1ng/mL、0 ng/mL)간예심기성섬유세포,분별우간예후5h、24h,채용RT-PCR검측I 형효원화Ⅳ형효원적표체.결과:1.용0ng/mL(대조조), 1ng/mL화10ng/mL적TGF-β1간예심기성섬유세포5h, RT-PCR검측I 형효원/β-actin광밀도치분별위0.61±0.02, 0.73±0.03 화 0.86±0.04,광밀도치수착TGF-β1농도적증가이증가,각조비교,차이유현저성(P＜0.01);간예심기성섬유세포24h후,RT-PCR검측I 형효원/β-actin광밀도치분별위0.65±.03, 0.91±0.02 화 0.98±0.02,광밀도치수착TGF-β1농도적증가이증가,각조비교,차이유현저성(P＜0.01).2.용0 ng/mL(대조조), 1ng/mL and 10ng/mL적TGF-β1간예심기성섬유세포5h, RT-PCR검측Ⅳ형효원/β-actin광밀도치분별위0.58±0.06, 0.71±0.02, 0.87±0.02,광밀도치수착TGF-β1농도적증가이증가,각조비교,차이유현저성(P＜0.01);간예심기성섬유세포24h후,RT-PCR검측Ⅳ형효원/β-actin광밀도치분별위0.62±0.01, 0.83±0.05, 0.96±0.02,광밀도치수착TGFβ1농도적증가이증가,각조비교,차이유현저성(P＜0.01).결론:TGFβ1능가강I형화Ⅳ형효원적표체,재24소시내정시간화제량의뢰성.
Objective To investigate the effect of TGF-β1 on synthesis of collagen I and collagen Ⅳ.Methods The cardiac fibroblasts wereisolated from SD mouse, which were treated using different concentrations of TGF-β1 ie.1ng/mL and 10ng/mL, then incubated for a specific duration of time.Cells were divided into three main groups(TGF-β1 10ng/mL, 1ng/mL and 0ng/mL).As stated above that all groups were observed for a given time(5 hrs or 24 hours).Then RT-PCR were used to detect biosynthesis of collagen type I and collagen Ⅳ respectively.Results 1.The primary cultured rat myocardial fibroblasts were treated using different concentrations of TGF-β1(0ng/mL, for control group; 1ng/mL and, and 10ng/mL for test groups respectively)after 5 hrs the OD values of collagen I/β-actin checked with RT-PCR were 0.61±0.02, 0.73±0.03 and 0.86±0.04 respectively, and after 24 h the OD values of collagen I/β-actin for control group, 1ng/ml and 10ng/mL were 0.65±0.03, 0.91±0.02 and 0.98±0.02 respectively(all P＜0.01 compared with controls, and other groups).And treated the cells with the same condition, the OD values for collagen Ⅳ/β-actin were 0.58±0.06, 0.71±0.02, 0.87±0.02 respectively, 24h the OD values of RT-PCR were 0.62±0.01, 0.83±0.05, 0.96±0.02(all P＜0.01 compared with controls, and other groups)respectively.Conclusion TGF-β1 can enhance biosynthesis of collagen I and Ⅳ in a dose and time dependent manner during 24 hours observation period.
