CAJ | 학술논문

背景:膜部和嵴内型室间隔缺损周围结构比较复杂,进行封堵治疗存在一定的难度,因此确定最佳干预方法需要建屯相应的动物模型.目的:在超声心图定位F建立室间隔膜部及嵴内型缺损动物模型.设计:动物造模实验.单位:复旦大学附属中山医院心内科.材料:选用健康成年草狗6只,雌雄不拘,从上海市实验动物中心购置.实验用主动脉-大隐静脉近端吻合器-打孔器为美国St.Jude公司产品,并对其进行改良即将其头部的塑料外壳成分削平、薄,也可把塑料外壳削短,使通过荷包进右室穿刺室间隔时阻力减少.PhilipsSonos5500多功能超声诊断仪由美国菲利普公司生产,经胸超声探头频率为2.5～3.5 MHz.方法:实验于2006-04/07在复旦大学附属中山医院、上海市心血管病研究所中心实验室完成(生物级别:BSL2级).将6只草狗麻醉后,取卧位,在超声心动图定位下,使用主动脉-大隐静脉近端吻合器-打孔器对室间隔穿刺,制备室间隔缺损.实验中动物处置符合复旦大学实验动物伦理委员会要求.主要观察指标:术后1周行超声诊断仪检查室间隔缺损位置、最大分流速度,跨室间隔缺损处压差,肺动脉压力.结果:成活的3只草狗成功建立了室间隔缺损模犁并进入结果分析.采用超声诊断仪于术后1周测得2只为嵴内型室间隔缺损,1只为膜部室间隔缺损:室间隔缺损直径1.8～3.6 mm.测得室间隔缺损处最大分流速度为1.8～4.0m/s:跨室间隔缺损处压差42～51 mmHg:肺动脉收缩压23～29mmHg.结论:在超声心动图定位下采用改良的主动脉-大隐静脉近端打孔器对室间隔穿刺可成功建立嵴内型/膜部室间隔缺损.
배경:막부화척내형실간격결손주위결구비교복잡,진행봉도치료존재일정적난도,인차학정최가간예방법수요건둔상응적동물모형.목적:재초성심도정위F건립실간격막부급척내형결손동물모형.설계:동물조모실험.단위:복단대학부속중산의원심내과.재료:선용건강성년초구6지,자웅불구,종상해시실험동물중심구치.실험용주동맥-대은정맥근단문합기-타공기위미국St.Jude공사산품,병대기진행개량즉장기두부적소료외각성분삭평、박,야가파소료외각삭단,사통과하포진우실천자실간격시조력감소.PhilipsSonos5500다공능초성진단의유미국비리보공사생산,경흉초성탐두빈솔위2.5～3.5 MHz.방법:실험우2006-04/07재복단대학부속중산의원、상해시심혈관병연구소중심실험실완성(생물급별:BSL2급).장6지초구마취후,취와위,재초성심동도정위하,사용주동맥-대은정맥근단문합기-타공기대실간격천자,제비실간격결손.실험중동물처치부합복단대학실험동물윤리위원회요구.주요관찰지표:술후1주행초성진단의검사실간격결손위치、최대분류속도,과실간격결손처압차,폐동맥압력.결과:성활적3지초구성공건립료실간격결손모리병진입결과분석.채용초성진단의우술후1주측득2지위척내형실간격결손,1지위막부실간격결손:실간격결손직경1.8～3.6 mm.측득실간격결손처최대분류속도위1.8～4.0m/s:과실간격결손처압차42～51 mmHg:폐동맥수축압23～29mmHg.결론:재초성심동도정위하채용개량적주동맥-대은정맥근단타공기대실간격천자가성공건립척내형/막부실간격결손.
BACKGROUND:Surrounding structure of intracristal ventricular septal defects(IVSD) and membranous ventricular septal defects(MVSD)is complicated,and it is difficult to perform transcatheter closure treatment.Corresponding animal models should be established to define an optimal interventional therapy.OBJECTIVE:To prepare the animal models of IVSD and MVSD under echoeardiogram location.DESIGN:Animal modeling experiment.SETTING:Department of Cardiology,Zhongshan Hospital Affiliated to Fudan University.MATERIALS:Six healthy adult dogs,irrespective of gender,were purchased from Shanghai Experimental Animal Center.Aorta and great saphenous vein stapler-puncher(St.Jude corporation,USA)was used.The main improvement is to cut the plastic shell even,thin,or shorter in order to reduce the resistance when puncturing the"purse"in the fight ventficle and the interventficular septum.Sonos 5500 multifunction ultrasonoscope(Philips,USA)was used,and the frequency of the probe was between 2.5-3.5 MHz.METHODS:Experiments were performed at the Zhongshan Hospital Affiliated to Fudan University and Central Laboratory of Shanghai Institute of Cardiovascular Disease from April to July 2006(biosaflety level 2).After anaesthesia,6 adult dogs were fixed at decumbent position.The interventricular septum was punetured to make the preparation with the location of echocardiography(ECHO)by using the modified aorta and great saphenous vein proximal stapler-puncher.Animal intervention met the Animal Ethical Committee of Fudan University.MAIN OUTCOME MEASURES:One week after the surgery,transthoracic echocardiography examination was used to check the VSD site,the highest shunt flow rate,the pressure difference between both sides of the VSD,as well as pulmonary artenal pressure.RESULTS:The VSD models were successfully established in three survivals and included in the final result.Two models of IVSD and one model of MVSD were examined by ECHO one week after the surgery.The diameter of VSD ranges from 1.8 to 3.6 mm.The Peak Flow Rate of the VSD ranges between 1.8 and 4.0 m/s.The pressure difierence of both sides of the VSD ranges between 42 and 51 mm Hg.The pulmonary arterial systolic pressure ranges from 23 to 29 mm Hg.CoNCLUSION:Located by the ECHO,it is possible to establish animal models of the IVSD and MVSD with the modified aorta and great saphenous vein proximal stapler-puncher.