中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2009年
10期
1-4
,共4页
余州%张晓燕%刁美玲%王勇%唐爱发%桂耀庭
餘州%張曉燕%刁美玲%王勇%唐愛髮%桂耀庭
여주%장효연%조미령%왕용%당애발%계요정
基因芯片%CatSper基因家族%小鼠%人类
基因芯片%CatSper基因傢族%小鼠%人類
기인심편%CatSper기인가족%소서%인류
gene chip%CatSper gene family%mice%human
目的 研究CatSper基因家族在人和小鼠组织中的分布特点.方法 将不同发育阶段的小鼠睾丸组织Cdna与Affymetrix全基因组芯片探进行杂交,筛选出差异表达基因CatSper基因家族.RT-PCR验证差异表达基因在不同发育阶段的小鼠睾丸组织的表达特征,及其在人和小鼠不同组织中的分布.结果 基因芯片分析发现CatSper1、CatSper2和CatSper3在小鼠睾丸的表达呈阶段特异性.RT-PCR结果 表明该基因家族在睾丸的表达丰度明显高于其它组织:CatSper1和CatSper4在人体睾丸组织中特异性表达.结论 CatSper基因家族在人和小鼠中呈现睾丸特异性表达或高表达,可能在精子发生中发挥重要功能.
目的 研究CatSper基因傢族在人和小鼠組織中的分佈特點.方法 將不同髮育階段的小鼠睪汍組織Cdna與Affymetrix全基因組芯片探進行雜交,篩選齣差異錶達基因CatSper基因傢族.RT-PCR驗證差異錶達基因在不同髮育階段的小鼠睪汍組織的錶達特徵,及其在人和小鼠不同組織中的分佈.結果 基因芯片分析髮現CatSper1、CatSper2和CatSper3在小鼠睪汍的錶達呈階段特異性.RT-PCR結果 錶明該基因傢族在睪汍的錶達豐度明顯高于其它組織:CatSper1和CatSper4在人體睪汍組織中特異性錶達.結論 CatSper基因傢族在人和小鼠中呈現睪汍特異性錶達或高錶達,可能在精子髮生中髮揮重要功能.
목적 연구CatSper기인가족재인화소서조직중적분포특점.방법 장불동발육계단적소서고환조직Cdna여Affymetrix전기인조심편탐진행잡교,사선출차이표체기인CatSper기인가족.RT-PCR험증차이표체기인재불동발육계단적소서고환조직적표체특정,급기재인화소서불동조직중적분포.결과 기인심편분석발현CatSper1、CatSper2화CatSper3재소서고환적표체정계단특이성.RT-PCR결과 표명해기인가족재고환적표체봉도명현고우기타조직:CatSper1화CatSper4재인체고환조직중특이성표체.결론 CatSper기인가족재인화소서중정현고환특이성표체혹고표체,가능재정자발생중발휘중요공능.
Objective To analyze the expression characteristics of CatSper gene family in human and mouse tissues. Methods Testis cDNA samples from Balb/C mice of different postnatal days were performed with mouse whole genome Affymetrix Genechip to screen differentially expressed genes. CatSper gene family screened by Genechip were further analysed by RT-PCR for their stage-specific and tissue-specific expression characteristics. Results The distinct expression of CatSperl, CatSperl and CatSper3 in developmental mouse testis was stage-specific. The abundance of CatSper gene family was higher in testis than any other tissues that we had detected. And CatSperl and CatSper4 were exclusively expressed in human testis. Conclusion CatSper gene family whose expression were Testis-specific or testis-enriched may play a vital role in human and mouse spermatogenesis.
