CAJ | 학술논문

通过使用市售的常规C18液相色谱柱(规格:150mm×4.6mm,5 μm),探讨了早熟素Ⅱ的分析方法并对其进行了可行性论证.以V(甲醇):V(水)=80:20为流动相,流量1 mL·min~(-1),检测波长为224nm,柱温35℃.得出早熟素Ⅱ在8～108μg·mL~(-1)的范围内呈良好的线性关系(相关系数,为0.99997),最低检出限为1.0379μg·mL~(-1)(信噪比为3),加标回收率为93.3%～104.98%,变异系数为0.12%～4.41%.与使用非常规色谱柱分析早熟索Ⅱ的方法相比,该方法采用了市售常规柱,能够更大限度地满足实验条件,通用性更强.
통과사용시수적상규C18액상색보주(규격:150mm×4.6mm,5 μm),탐토료조숙소Ⅱ적분석방법병대기진행료가행성론증.이V(갑순):V(수)=80:20위류동상,류량1 mL·min~(-1),검측파장위224nm,주온35℃.득출조숙소Ⅱ재8～108μg·mL~(-1)적범위내정량호적선성관계(상관계수,위0.99997),최저검출한위1.0379μg·mL~(-1)(신조비위3),가표회수솔위93.3%～104.98%,변이계수위0.12%～4.41%.여사용비상규색보주분석조숙색Ⅱ적방법상비,해방법채용료시수상규주,능구경대한도지만족실험조건,통용성경강.
A method for determination of precocene Ⅱ was developed by HPLC with conventional C18 column(150 mm×4.6 mm, 5 um)and its feasibility assessment was made. The samples were analyzed on a Luna C18 column using methyl alcohol- ultrapure water (80 : 20, V/V) as the mobile phase at a flow rate of 1 mL·min~(-1). The detection wavelength was 224 nm and the column tem-perature was 35 ℃. Well linear relationship was showed at the range of 8～108 μg·mL~(-1) and correlation coefficient of the calibration curve was 0.999 97. The detection limit was 1.037 9 μg·mL~(-1) (S/N=3). The recovery of added sample ranged from 93.3% to 104.98% with the relative standard deviations of 0.12%～4.41%. Comparing with other measuring methods using unconventional column, better versatility was manifested by the method in which a conventional liquid chromatographic column which was easily available in the market was employed and expetimental conditions were met to a great extent.