CAJ | 학술논문

目的:研究百草枯诱导的氧化胁迫在涡虫严重致死率发生中的角色.方法:动物的存活率用于反映涡虫的致死率.百草枯用来诱导严重的氧化胁迫,氧化胁迫的发生程度通过过氧化物酶(SOD)的活性变化来监测.游离氧自由基清除剂DMSO用来在百草枯处理前或后处理涡虫.结果:浓度为2 mmol·L-1百草枯处理1 h显著降低SOD活性,而2 mmol·L-1百草枯处理2、4、8 h可以导致更严重的SOD活性降低.暴露2 h后,浓度为1 mmol·L-1百草枯不明显影响SOD活性,而浓度为2 、3与4 mmol·L-1的百草枯处理则显著降低SOD活性.同时,经浓度为2、3与4 mmol·L-1百草枯处理后,继续发育3 d的涡虫存活率发生了显著的降低.进而,对浓度为2 mmol·L-1百草枯处理2、4与8 h涡虫进行预先或延后DMSO(0.1%)处理4 h可以显著增加百草枯处理动物的SOD活性.尽管如此,在2 mmol·L-1百草枯处理2、4与8 h基础上再进行DMSO(0.1%)预先或延后处理4 h涡虫动物的SOD活性尚不能够达到正常涡虫中的SOD活性水平.而且,对浓度为2 mmol·L-1百草枯处理2、4与8 h涡虫进行预先或延后DMSO(0.1%)处理4 h还可以显著提高百草枯处理动物的存活率.结论:DMSO预先或延后处理可以有效地预防或恢复百草枯暴露涡虫中经氧化胁迫诱导的严重致死率毒害.
목적:연구백초고유도적양화협박재와충엄중치사솔발생중적각색.방법:동물적존활솔용우반영와충적치사솔.백초고용래유도엄중적양화협박,양화협박적발생정도통과과양화물매(SOD)적활성변화래감측.유리양자유기청제제DMSO용래재백초고처리전혹후처리와충.결과:농도위2 mmol·L-1백초고처리1 h현저강저SOD활성,이2 mmol·L-1백초고처리2、4、8 h가이도치경엄중적SOD활성강저.폭로2 h후,농도위1 mmol·L-1백초고불명현영향SOD활성,이농도위2 、3여4 mmol·L-1적백초고처리칙현저강저SOD활성.동시,경농도위2、3여4 mmol·L-1백초고처리후,계속발육3 d적와충존활솔발생료현저적강저.진이,대농도위2 mmol·L-1백초고처리2、4여8 h와충진행예선혹연후DMSO(0.1%)처리4 h가이현저증가백초고처리동물적SOD활성.진관여차,재2 mmol·L-1백초고처리2、4여8 h기출상재진행DMSO(0.1%)예선혹연후처리4 h와충동물적SOD활성상불능구체도정상와충중적SOD활성수평.이차,대농도위2 mmol·L-1백초고처리2、4여8 h와충진행예선혹연후DMSO(0.1%)처리4 h환가이현저제고백초고처리동물적존활솔.결론:DMSO예선혹연후처리가이유효지예방혹회복백초고폭로와충중경양화협박유도적엄중치사솔독해.
Objective: To investigate the roles of oxidative stress induced by paraquat treatment in the formation of severe of lethality in planarian Dugesia japonica. Methods: The percentage of survival animals was used to reflect the lethality. The paraquat was used to induce the formation of severe oxidative stress, which was monitored by the superoxide dismutase(SOD) activity. DMSO, an effective free radical scavenger, was used to treat the planarians before or after the paraquat treatment. Results: The significant decrease of SOD activity was observed in planarians exposed to 2 mmol·L-1 of paraquat for 1 h, and more severe decreases of SOD activity were further detected in planarians exposed to 2 mmol·L-1 of paraquat for 2, 4, and 8 h. After 2 h exposure, 1 mmol·L-1 of paraquat treatment did not induce an obvious alteration of SOD activity;however, the significant decreases of SOD activities were observed in paraquat exposed planarians at the concentrations from 2 mmol·L-1 to 4 mmol·L-1. Moreover, three days after the paraquat treatment, the significant decreases of percentages of survival animals were observed in planarians exposed to 2, 3, and 4 mmol·L-1 of paraquat. Furthermore, the significant increases of SOD activities were detected in 2 mmol·L-1 paraquat exposed planarians for 2, 4, and 8 h pre-or post-treated with 0.1% DMSO for 4h compared with those in 2 mmol·L-1 paraquat exposed planarians for 2, 4, and 8h, respectively. Nevertheless, the values of SOD activities in 2 mmol·L-1 paraquat exposed planarians for 2, 4, and 8 h pre-or post-treated with 0.1% DMSO for 4h could not reach the value of SOD activity in planarians without paraquat treatment. The significant increase of percentage of survival animals was further observed in 2 mmol·L-1 paraquat exposed planarians for 2 h pre-or post-treated with 0.1% DMSO for 4 h compared with that in 2 mmol·L-1 paraquat exposed planarians for 2 h. Conclusion: These data suggest that pre-or post-treatment with DMSO can effectively prevent or retrieve the formation of oxidative stress-induced severe lethality in paraquat-treated planarians.