CAJ | 학술논문

利用SRAP分子标记技术,对采自中国西南5省区的44份野生狗牙根及8份非洲狗牙根材料进行遗传多样性研究.结果表明,18对引物组合共得到扩增总条带236条,多态性条带数206条,平均每对引物扩增出11.4条带,多态性位点百分率为87.29%,材料间的遗传相似系数范围为0.569～0.929,平均GS值为0.723.聚类分析结果表明,52份材料可聚为5类;基于Shannon多样性指数估算了8个狗牙根生态地理类群内和类群间的遗传分化,类群内的遗传变异占总变异的63.81%,类群间的遗传变异占总变异的36.19%,表明这些抗源材料遗传差异较大,各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性.
이용SRAP분자표기기술,대채자중국서남5성구적44빈야생구아근급8빈비주구아근재료진행유전다양성연구.결과표명,18대인물조합공득도확증총조대236조,다태성조대수206조,평균매대인물확증출11.4조대,다태성위점백분솔위87.29%,재료간적유전상사계수범위위0.569～0.929,평균GS치위0.723.취류분석결과표명,52빈재료가취위5류;기우Shannon다양성지수고산료8개구아근생태지리류군내화류군간적유전분화,류군내적유전변이점총변이적63.81%,류군간적유전변이점총변이적36.19%,표명저사항원재료유전차이교대,각생태지리류군간적유전분화여기소처적생태지리배경구유일정적상관성.
Sequence-related amplified polymorphism (SRAP) markers were used to detect the genetic diversity of 44 wild accessions of Cynodon dactylon collected from Sichuan, Chongqing,Yunnan, Guizhou and Tibet of China, and 8 wild accessions of C. dactylon collected from Africa. The results showed that eighteen primer pairs produced 206 polymorphic bands, averaged 11.4 bands per primer pair. The percentage of polymorphic bands in average was 87.29%.The Nei's genetic similarity coefficient of the tested accessions ranged from 0.569 to 0.929, and the average Nei's coefficient was 0.723.The 54 accessions were classified into five major groups: Ⅰ, Ⅱ, Ⅲ, Ⅳand Ⅴ by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Genetic differentiation between and within eight eco-geographical groups of C. dactylon was estimated by Shannon's diversity index, which showed that 63.81% genetic variance existed within group, and 36.19% genetic variance was among groups, indicating rather large genetic distances among those germplasm,and there was a correlation between genetic differentiation and eco-geographical habits among the groups.