CAJ | 학술논문

本文采用富集筛选法,以1-氨基环丙烷-1-羧酸（1-aminocyclopropane-1-carboxylate,ACC）为唯一氮源,从白城农科院实验地燕麦根际土壤中分离出2株具有ACC脱氨酶活性的细菌。其中菌株7ACC9-1的ACC脱氨酶酶活性较高,并用此菌株处理燕麦种子,在不同浓度的碳酸氢钠溶液的碱胁迫下进行燕麦种子的萌发实验和初生苗的生长试验,测定燕麦种子的发芽率、燕麦初生苗的根长和苗高。结果表明：燕麦种子用菌株处理后,发芽率显着提高;同时,在各浓度的碱胁迫下,燕麦处理组的初生苗根长和苗高均显着地优于对照组。说明具ACC脱氨酶活性的7ACC9-1能够有效地缓解燕麦种子在盐碱胁迫下萌发及其在初生苗生长阶段所受到的危害。
본문채용부집사선법,이1-안기배병완-1-최산（1-aminocyclopropane-1-carboxylate,ACC）위유일담원,종백성농과원실험지연맥근제토양중분리출2주구유ACC탈안매활성적세균。기중균주7ACC9-1적ACC탈안매매활성교고,병용차균주처리연맥충자,재불동농도적탄산경납용액적감협박하진행연맥충자적맹발실험화초생묘적생장시험,측정연맥충자적발아솔、연맥초생묘적근장화묘고。결과표명：연맥충자용균주처리후,발아솔현착제고;동시,재각농도적감협박하,연맥처리조적초생묘근장화묘고균현착지우우대조조。설명구ACC탈안매활성적7ACC9-1능구유효지완해연맥충자재염감협박하맹발급기재초생묘생장계단소수도적위해。
Two PGPR strains with ACC deaminase activity was isolated in the soil of oat roots in BaiCheng Academy of Agricultural Science by using enriching and screening method which took the 1-aminocyclopropane-1-carboxylate as the only source of the nitrogen.The 7ACC9-2＇s ACC deaminase activity is higher than another,and it was used to treat the oat seeds,which were used in the experiment of the oat seeds germination and growth under different concentration sodium bicarbonate,measuring seed germination rate,seedling height and root length.The results showed that the germination rate was significantly increased by 7ACC9-2,and seedling height and root length are also significantly better than check group under different concentration sodium bicarbonate.The 7ACC9-2 can effectively solve the problem of the salinization of oat seed germination and harms of growth stage.