国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2011年
5期
261-265
,共5页
高分辨熔解分析%突变%临床应用%Citrin缺陷病
高分辨鎔解分析%突變%臨床應用%Citrin缺陷病
고분변용해분석%돌변%림상응용%Citrin결함병
High resolution melting analysis%Mutation%Clinical application%Citrin deficiency
高分辨率熔解分析(high resolution melting analysis,HRMA)是一种研究单核苷酸多态性及DNA突变的新技术.HRMA作为一种简便、准确、快速、低成本的闭管操作模式,已在DNA突变扫描、基因分型、甲基化检测与序列匹配等临床研究中得到应用.该文就HRMA的技术特点及其临床应用作一综述.
高分辨率鎔解分析(high resolution melting analysis,HRMA)是一種研究單覈苷痠多態性及DNA突變的新技術.HRMA作為一種簡便、準確、快速、低成本的閉管操作模式,已在DNA突變掃描、基因分型、甲基化檢測與序列匹配等臨床研究中得到應用.該文就HRMA的技術特點及其臨床應用作一綜述.
고분변솔용해분석(high resolution melting analysis,HRMA)시일충연구단핵감산다태성급DNA돌변적신기술.HRMA작위일충간편、준학、쾌속、저성본적폐관조작모식,이재DNA돌변소묘、기인분형、갑기화검측여서렬필배등림상연구중득도응용.해문취HRMA적기술특점급기림상응용작일종술.
High resolution melting analysis (HRMA) is a novel technique developed in recent years for detection of single nucleotide polymorphisms ( SNPs ) and DNA mutations.Nowadays HRMA has been applied into clinical research such as DNA variant scanning,genotyping,methylation analysis and sequence matching,as an inexpensive closed tube format that is convenient,accurate and rapid.The technical features of HRMA and its clinical application were reviewed in this paper.
