中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
9期
790-793
,共4页
张锟%何方军%白祥军%高伟%尹燕华%李占飞
張錕%何方軍%白祥軍%高偉%尹燕華%李佔飛
장곤%하방군%백상군%고위%윤연화%리점비
创伤和损伤%髓源抑制细胞%感染%免疫功能障碍
創傷和損傷%髓源抑製細胞%感染%免疫功能障礙
창상화손상%수원억제세포%감염%면역공능장애
Wounds and injuries%Myeloid-derived suppressor cell%Infection%Immune dysfunction
目的 探讨严重创伤患者外周血中髓源抑制细胞(MDSCs)数量变化及其临床意义.方法 收集34例多发伤患者和7例健康志愿者外周血,以CD14-/CD11b+/CD33+作为MDSCs标志物,用流式细胞术(FCM)检测MDSCs所占比例,ELISA检测血清IL-10和C反应蛋白水平,分析MDSCs数量的变化情况及其临床意义. 结果对照组外周血MDSCs占(1.13±0.25)%,感染组多发伤患者伤后1,2,3和7 d外周血MDSCs所占比例分别为(1.20±0.22)%、(6.44±0.35)%、(13.84±2.07)%和(15.60±1.63)%,非感染组患者伤后1,2,3和7 d外周血MDSCs所占比例分别为(1.29±0.30)%、(4.93±0.32)%、(5.15±0.21)%和(3.77±0.34)%.两组2,3和7 d时MDSCs比例差异有统计学意义(P＜0.05).MDSCs比例与ISS、血清IL-10和C反应蛋白水平无相关性. 结论多发伤伤患者外周血MDSCs比例升高与并发感染相关,提示它可能在创伤后免疫功能障碍中起重要作用.
目的 探討嚴重創傷患者外週血中髓源抑製細胞(MDSCs)數量變化及其臨床意義.方法 收集34例多髮傷患者和7例健康誌願者外週血,以CD14-/CD11b+/CD33+作為MDSCs標誌物,用流式細胞術(FCM)檢測MDSCs所佔比例,ELISA檢測血清IL-10和C反應蛋白水平,分析MDSCs數量的變化情況及其臨床意義. 結果對照組外週血MDSCs佔(1.13±0.25)%,感染組多髮傷患者傷後1,2,3和7 d外週血MDSCs所佔比例分彆為(1.20±0.22)%、(6.44±0.35)%、(13.84±2.07)%和(15.60±1.63)%,非感染組患者傷後1,2,3和7 d外週血MDSCs所佔比例分彆為(1.29±0.30)%、(4.93±0.32)%、(5.15±0.21)%和(3.77±0.34)%.兩組2,3和7 d時MDSCs比例差異有統計學意義(P＜0.05).MDSCs比例與ISS、血清IL-10和C反應蛋白水平無相關性. 結論多髮傷傷患者外週血MDSCs比例升高與併髮感染相關,提示它可能在創傷後免疫功能障礙中起重要作用.
목적 탐토엄중창상환자외주혈중수원억제세포(MDSCs)수량변화급기림상의의.방법 수집34례다발상환자화7례건강지원자외주혈,이CD14-/CD11b+/CD33+작위MDSCs표지물,용류식세포술(FCM)검측MDSCs소점비례,ELISA검측혈청IL-10화C반응단백수평,분석MDSCs수량적변화정황급기림상의의. 결과대조조외주혈MDSCs점(1.13±0.25)%,감염조다발상환자상후1,2,3화7 d외주혈MDSCs소점비례분별위(1.20±0.22)%、(6.44±0.35)%、(13.84±2.07)%화(15.60±1.63)%,비감염조환자상후1,2,3화7 d외주혈MDSCs소점비례분별위(1.29±0.30)%、(4.93±0.32)%、(5.15±0.21)%화(3.77±0.34)%.량조2,3화7 d시MDSCs비례차이유통계학의의(P＜0.05).MDSCs비례여ISS、혈청IL-10화C반응단백수평무상관성. 결론다발상상환자외주혈MDSCs비례승고여병발감염상관,제시타가능재창상후면역공능장애중기중요작용.
Objective To explore the quantity change and significance of CD14-/CD11b+/CD33 + myeloid-derived suppressor cells (MDSCs) in patients with multiple injury. Methods Thirtyfour patients with multiple injury and seven healthy volunteers were enrolled in this study. Peripheral blood was collected and the factors of CD14-/CD1 1 b+/ CD33 + were taken as markers of MDSCs. The percentage of MDSCs was determined by flow cytometry (FCM) and serum interleukin-10 and C-reactive protein levels were determined by ELISA to analyze the quantity change and clinical significance of MDSCs. Results The percentage of MDSCs in peripheral blood of healthy volunteers was (1.13 +0. 25) %. At days 1,2, 3 and 7 after injury, the percentage of MDSCs in peripheral blood were (1.20 +0.22) %, (6.44 + 0.35) %, (13.84 ± 2.07) % and (15.60 ± 1.63) % respectively in patients with infection and multiple injury, whereas (1.29 ±0. 30)%, (4.93 +0. 32)%, (5.15 ±0. 21)% and (3.77 ± 0.34) % respectively in patients without infection. The percentages of MDSCs in two groups showed significant differences at days 2, 3 and 7 after trauma (P＜0.05). No correlation was found between MDSCs percentage in peripheral blood and injury severity score, serum interleukin-10 or C reactive protein in patients with multiple injury (P ＞ 0.05). Conclusions The increase of proportion ofMDSCs in peripheral blood correlates with the onset of infection in patients with multiple injury, indicating that the expansion of MDSCs in peripheral blood may play important roles in immune dysfunction after multiple injury.
