中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2011年
4期
328-332
,共5页
范一宏%张高松%李延玲%张璐%吕宾
範一宏%張高鬆%李延玲%張璐%呂賓
범일굉%장고송%리연령%장로%려빈
胶质细胞源性神经营养因子%丝裂原激活蛋白激酶类%Akt%慢传输型便秘
膠質細胞源性神經營養因子%絲裂原激活蛋白激酶類%Akt%慢傳輸型便祕
효질세포원성신경영양인자%사렬원격활단백격매류%Akt%만전수형편비
Glial cell line-derived neurotrophic factor%Mitogen-activated protein kinases%Akt%Slow transit constipation
目的 研究慢传输型便秘(STC)大鼠胃、结肠中Akt、MAPK的表达及外源性胶质细胞源性神经营养因子(GDNF)对其的影响,并探讨GDNF对STC大鼠的作用途径及其信号传导机制.方法 成年SD大鼠44只,随机分为对照组和模型组.对照组用生理盐水灌胃;模型组用大黄灌胃,共3.5个月,建立STC大鼠模型.建模成功后,对照组分为正常对照亚组和GDNF亚组,模型组分为STC亚组和STC+GDNF亚组,GDNF亚组和STC+GDNF亚组给予外源性GDNF 1周.采用免疫组化法检测大鼠胃、结肠组织中Akt和MAPK的表达情况.结果 (1)胃组织中,STC亚组的Akt表达水平较正常对照亚组有减弱趋势(P＞0.05);STC+GDNF亚组较STC亚组增强(P＜0.05);STC+GDNF亚组与正常对照亚组、GDNF亚组比较,Akt表达水平的差异无统计学意义(P＞0.05).(2)结肠组织中,STC组的Akt、MAPK表达水平均较正常对照亚组减弱(P＜0.05),而在STC+GDNF亚组表达均增强(P＜0.05).(3)胃组织中,STC亚组的MAPK表达水平较正常对照亚组减弱(P＜0.05);STC+GDNF亚组较STC亚组表达增强(P＜0.01);STC+GDNF亚组与正常对照亚组、GDNF亚组比较,MAPK表达水平的差异无统计学意义(P＞0.05).结论 长期使用大黄可使大鼠的胃和结肠组织中的Akt和MAPK表达减少,这可能是STC的发病机制之一.外源性GDNF有可能通过提高STC大鼠胃和结肠组织中Akt和MAPK的表达而保护消化道中的神经元.
目的 研究慢傳輸型便祕(STC)大鼠胃、結腸中Akt、MAPK的錶達及外源性膠質細胞源性神經營養因子(GDNF)對其的影響,併探討GDNF對STC大鼠的作用途徑及其信號傳導機製.方法 成年SD大鼠44隻,隨機分為對照組和模型組.對照組用生理鹽水灌胃;模型組用大黃灌胃,共3.5箇月,建立STC大鼠模型.建模成功後,對照組分為正常對照亞組和GDNF亞組,模型組分為STC亞組和STC+GDNF亞組,GDNF亞組和STC+GDNF亞組給予外源性GDNF 1週.採用免疫組化法檢測大鼠胃、結腸組織中Akt和MAPK的錶達情況.結果 (1)胃組織中,STC亞組的Akt錶達水平較正常對照亞組有減弱趨勢(P＞0.05);STC+GDNF亞組較STC亞組增彊(P＜0.05);STC+GDNF亞組與正常對照亞組、GDNF亞組比較,Akt錶達水平的差異無統計學意義(P＞0.05).(2)結腸組織中,STC組的Akt、MAPK錶達水平均較正常對照亞組減弱(P＜0.05),而在STC+GDNF亞組錶達均增彊(P＜0.05).(3)胃組織中,STC亞組的MAPK錶達水平較正常對照亞組減弱(P＜0.05);STC+GDNF亞組較STC亞組錶達增彊(P＜0.01);STC+GDNF亞組與正常對照亞組、GDNF亞組比較,MAPK錶達水平的差異無統計學意義(P＞0.05).結論 長期使用大黃可使大鼠的胃和結腸組織中的Akt和MAPK錶達減少,這可能是STC的髮病機製之一.外源性GDNF有可能通過提高STC大鼠胃和結腸組織中Akt和MAPK的錶達而保護消化道中的神經元.
목적 연구만전수형편비(STC)대서위、결장중Akt、MAPK적표체급외원성효질세포원성신경영양인자(GDNF)대기적영향,병탐토GDNF대STC대서적작용도경급기신호전도궤제.방법 성년SD대서44지,수궤분위대조조화모형조.대조조용생리염수관위;모형조용대황관위,공3.5개월,건립STC대서모형.건모성공후,대조조분위정상대조아조화GDNF아조,모형조분위STC아조화STC+GDNF아조,GDNF아조화STC+GDNF아조급여외원성GDNF 1주.채용면역조화법검측대서위、결장조직중Akt화MAPK적표체정황.결과 (1)위조직중,STC아조적Akt표체수평교정상대조아조유감약추세(P＞0.05);STC+GDNF아조교STC아조증강(P＜0.05);STC+GDNF아조여정상대조아조、GDNF아조비교,Akt표체수평적차이무통계학의의(P＞0.05).(2)결장조직중,STC조적Akt、MAPK표체수평균교정상대조아조감약(P＜0.05),이재STC+GDNF아조표체균증강(P＜0.05).(3)위조직중,STC아조적MAPK표체수평교정상대조아조감약(P＜0.05);STC+GDNF아조교STC아조표체증강(P＜0.01);STC+GDNF아조여정상대조아조、GDNF아조비교,MAPK표체수평적차이무통계학의의(P＞0.05).결론 장기사용대황가사대서적위화결장조직중적Akt화MAPK표체감소,저가능시STC적발병궤제지일.외원성GDNF유가능통과제고STC대서위화결장조직중Akt화MAPK적표체이보호소화도중적신경원.
Objective To study the expression of Akt and MAPK in the stomach and colon of slow transit constipation (STC) in rats, as well as the effect of exogenous glial cell line-derived neurotrophic factor (GDNF) on it. Methods Forty-four SD rats were divided into control group and model group randomly. The STC model group was established by gastric irrigation of rhubarb for 3.5 months. The control group was received normal saline. After model building, each group was equally divided into 2 subgroup randomly, administrated with exogenous GDNF and normal saline by vein injection for one week respectively. The expression of Akt and MAPK in stomach and colon was detected by immunohistochemistry.Results ( 1 ) The expression of Akt in the stomach tended to weaker in STC rats comparing with the normal rats ( P ＞ 0. 05 ), but it was stronger in STC plus GDNF group than in STC group ( P ＜ 0. 05 ). ( 2 ) The expression of Akt and MAPK in the colon was weaker in STC group than in the normal group ( all P ＜0. 05 ), and was stronger in STC plus GDNF group than in STC group ( all P ＜ 0. 05 ). ( 3 ) The expression of MAPK in the stomach in STC group was weaker than in normal group (P ＜ 0.05 ), and was stronger in STC plus GDNF group than in STC group (P ＜0.01 ). There was no significant difference among STC plus GDNF group, normal group and GDNF group (P ＞ 0. 05 ). Conclusions Long term consumption of rhubarb could induce STC by down-regulating the expression of Akt and MAPK in digestive tract. Exogenous GDNF may have a potential role on the etiology of STC.
