CAJ | 학술논문

目的建立不同胰岛素敏感状态的细胞模型,研究不同状态下蛋白激酶B(PKB)的表达. 方法对3T3-L1脂肪细胞采用不同浓度葡萄糖(3.0 mmol/L、5.5 mmol/L和50.0 mmol/L)进行培养,应用胰岛素介导的3H-2脱氧-D葡萄糖摄取率,评价低糖组、对照组和高糖组3T3-L1脂肪细胞的胰岛素敏感性,并以RT-PCR方法检测葡萄糖刺激前后各组细胞中PKB的表达. 结果 3T3-L1脂肪细胞3H-2脱氧-D葡萄糖摄取率依次为,低糖组0.80±0.28,对照组0.53±0.33,高糖组0.32±0.13;在相同条件下.3组细胞间的PKB mRNA表达差异无统计学意义;但在葡萄糖刺激后普遍降低,低糖组、对照组和高糖组分别降低了52%、46%和59%,差异有统计学意义(P<0.05). 结论体外不同的葡萄糖浓度培养条件,可产生3T3-L1脂肪细胞不同的胰岛素敏感状态,而细胞在此条件下的PKB表达并无明显差异.
목적 건립불동이도소민감상태적세포모형,연구불동상태하단백격매B(PKB)적표체. 방법 대3T3-L1지방세포채용불동농도포도당(3.0 mmol/L、5.5 mmol/L화50.0 mmol/L)진행배양,응용이도소개도적3H-2탈양-D포도당섭취솔,평개저당조、대조조화고당조3T3-L1지방세포적이도소민감성,병이RT-PCR방법검측포도당자격전후각조세포중PKB적표체. 결과 3T3-L1지방세포3H-2탈양-D포도당섭취솔의차위,저당조0.80±0.28,대조조0.53±0.33,고당조0.32±0.13;재상동조건하.3조세포간적PKB mRNA표체차이무통계학의의;단재포도당자격후보편강저,저당조、대조조화고당조분별강저료52%、46%화59%,차이유통계학의의(P<0.05). 결론 체외불동적포도당농도배양조건,가산생3T3-L1지방세포불동적이도소민감상태,이세포재차조건하적PKB표체병무명현차이.
Objective To establish cell models with different insulin sensitivity status and examine the expression of protein kinase B (PKB/Akt) in the same conditions. Methods 3T3-L1cells were cultured with different glucose concentrations (3.0, 5.5 and 50.0 mmol/L respectively).Insulin sensitivity, as presented by insulin-induced glucose transport rate in the cells, was tested by 3H-2-DG incorporation. PKB expression in cells was determined by RT-PCR. Results The insulin-induced glucose transport rate in 3T3-L1 cells was affected by different concentrations of glucose. The transport rate of 3H -2-DG in cells cultured in low glucose concentration was increased by 152% and 50% compared with high glucose concentration and control group, respectively. There was no difference in PKB mRNA expression among the three groups under the same conditions,whereas there was an obvious reduction of PKB expression in the three groups after the cells were treated with glucose and insulin. Conclusions 3T3-L1 cells cultured with different concentrations of glucose present the different insulin sensitivity status, but the difference of PKB mRNA expression has not been observed in the same condition.