国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2012年
1期
35-41
,共7页
吴晓牧%周鑫华%王卫真%王朝东%胡国柱%熊英琼%刘丽娟%张昆南
吳曉牧%週鑫華%王衛真%王朝東%鬍國柱%熊英瓊%劉麗娟%張昆南
오효목%주흠화%왕위진%왕조동%호국주%웅영경%류려연%장곤남
卒中%脑缺血%动脉粥样硬化%多态现象,遗传学%血管紧张素原%血管紧张素Ⅱ%受体,血管紧张素,1型%醛固酮合酶%高血压
卒中%腦缺血%動脈粥樣硬化%多態現象,遺傳學%血管緊張素原%血管緊張素Ⅱ%受體,血管緊張素,1型%醛固酮閤酶%高血壓
졸중%뇌결혈%동맥죽양경화%다태현상,유전학%혈관긴장소원%혈관긴장소Ⅱ%수체,혈관긴장소,1형%철고동합매%고혈압
Stroke%Brain Ischemia%Atherosclerosis%Polymorphism,Genetic%Angiotensinogen%Angiotensin Ⅱ%Receptor,Angiotensin,Type 1%Aldosterone Synthase%Hypertension
目的 研究肾素-血管紧张素-醛固酮系统血管紧张素原(angiotensinogen,AGT)基因M235T、血管紧张素Ⅱ1型受体(angiot ensinⅡtype 1 receptor,AGTR1)基因A1166C、醛固酮合酶( aldosterone synthase,CYP11B2)基因- 344C/T多态性与中国南方汉族人群大动脉粥样硬化性卒中(large-artery atherosclerosis,LAA)的相关性.方法 采用聚合酶链反应和基因测序技术对中国南方汉族LAA患者和正常对照者AGT基因M235T、ATGR1基因A1166C和CYP11B2基因- 344C/T多态性进行基因分型,并通过二分类logistic回归分析确定这3种基因多态性与LAA的相关性.结果 共纳入LAA患者107例和142名健康对照者.LAA组AGT基因235TT基因型(66.36%对50.70%,x2=6.122,P=0.047)和T等位基因(79.44%对70.07%,x2=5.581,P=0.018)频率显著高于对照组,AGTR1基因1166CC基因型(0%对0%,x2=1.494,P=0.222)和C等位基因(7.48%对4.93%,x2=1.399,P=0.237)频率与对照组无显著性差异,CYP11B2基因- 344CC基因型(9.35%对4.23%,x2=3.603,P=0.165)和C等位基因(27.10%对26.06%,x2=0.069,P=0.793)频率与对照组亦无显著性差异.二分类logistic回归分析显示,这3种基因多态性与单纯性LAA患病均无显著相关性.合并高血压的LAA患者AGT基因235TT基因型(68.00%对41.90%,x2=12.446,P=0.002)和T等位基因(79.33%对64.76%,x2=8.993,P=0.003)频率均显著高于血压正常对照组,logistic回归分析显示,暴露于TT基因型的优势比(odds ratio,OR)为2.153[ 95%可信区间(confidence interval,CI)0.789 ~5.872],T等位基因的OR值为2.089(95% CI1.285 ~3.396).结论 AGT基因M235T多态性与南方汉族人群单纯性LAA无关,但可能与合并高血压的LAA患病风险相关;CYP11B2基因- 344C/T多态性和AGTR1基因A1166C多态性与南方汉族人群LAA发病无关.
目的 研究腎素-血管緊張素-醛固酮繫統血管緊張素原(angiotensinogen,AGT)基因M235T、血管緊張素Ⅱ1型受體(angiot ensinⅡtype 1 receptor,AGTR1)基因A1166C、醛固酮閤酶( aldosterone synthase,CYP11B2)基因- 344C/T多態性與中國南方漢族人群大動脈粥樣硬化性卒中(large-artery atherosclerosis,LAA)的相關性.方法 採用聚閤酶鏈反應和基因測序技術對中國南方漢族LAA患者和正常對照者AGT基因M235T、ATGR1基因A1166C和CYP11B2基因- 344C/T多態性進行基因分型,併通過二分類logistic迴歸分析確定這3種基因多態性與LAA的相關性.結果 共納入LAA患者107例和142名健康對照者.LAA組AGT基因235TT基因型(66.36%對50.70%,x2=6.122,P=0.047)和T等位基因(79.44%對70.07%,x2=5.581,P=0.018)頻率顯著高于對照組,AGTR1基因1166CC基因型(0%對0%,x2=1.494,P=0.222)和C等位基因(7.48%對4.93%,x2=1.399,P=0.237)頻率與對照組無顯著性差異,CYP11B2基因- 344CC基因型(9.35%對4.23%,x2=3.603,P=0.165)和C等位基因(27.10%對26.06%,x2=0.069,P=0.793)頻率與對照組亦無顯著性差異.二分類logistic迴歸分析顯示,這3種基因多態性與單純性LAA患病均無顯著相關性.閤併高血壓的LAA患者AGT基因235TT基因型(68.00%對41.90%,x2=12.446,P=0.002)和T等位基因(79.33%對64.76%,x2=8.993,P=0.003)頻率均顯著高于血壓正常對照組,logistic迴歸分析顯示,暴露于TT基因型的優勢比(odds ratio,OR)為2.153[ 95%可信區間(confidence interval,CI)0.789 ~5.872],T等位基因的OR值為2.089(95% CI1.285 ~3.396).結論 AGT基因M235T多態性與南方漢族人群單純性LAA無關,但可能與閤併高血壓的LAA患病風險相關;CYP11B2基因- 344C/T多態性和AGTR1基因A1166C多態性與南方漢族人群LAA髮病無關.
목적 연구신소-혈관긴장소-철고동계통혈관긴장소원(angiotensinogen,AGT)기인M235T、혈관긴장소Ⅱ1형수체(angiot ensinⅡtype 1 receptor,AGTR1)기인A1166C、철고동합매( aldosterone synthase,CYP11B2)기인- 344C/T다태성여중국남방한족인군대동맥죽양경화성졸중(large-artery atherosclerosis,LAA)적상관성.방법 채용취합매련반응화기인측서기술대중국남방한족LAA환자화정상대조자AGT기인M235T、ATGR1기인A1166C화CYP11B2기인- 344C/T다태성진행기인분형,병통과이분류logistic회귀분석학정저3충기인다태성여LAA적상관성.결과 공납입LAA환자107례화142명건강대조자.LAA조AGT기인235TT기인형(66.36%대50.70%,x2=6.122,P=0.047)화T등위기인(79.44%대70.07%,x2=5.581,P=0.018)빈솔현저고우대조조,AGTR1기인1166CC기인형(0%대0%,x2=1.494,P=0.222)화C등위기인(7.48%대4.93%,x2=1.399,P=0.237)빈솔여대조조무현저성차이,CYP11B2기인- 344CC기인형(9.35%대4.23%,x2=3.603,P=0.165)화C등위기인(27.10%대26.06%,x2=0.069,P=0.793)빈솔여대조조역무현저성차이.이분류logistic회귀분석현시,저3충기인다태성여단순성LAA환병균무현저상관성.합병고혈압적LAA환자AGT기인235TT기인형(68.00%대41.90%,x2=12.446,P=0.002)화T등위기인(79.33%대64.76%,x2=8.993,P=0.003)빈솔균현저고우혈압정상대조조,logistic회귀분석현시,폭로우TT기인형적우세비(odds ratio,OR)위2.153[ 95%가신구간(confidence interval,CI)0.789 ~5.872],T등위기인적OR치위2.089(95% CI1.285 ~3.396).결론 AGT기인M235T다태성여남방한족인군단순성LAA무관,단가능여합병고혈압적LAA환병풍험상관;CYP11B2기인- 344C/T다태성화AGTR1기인A1166C다태성여남방한족인군LAA발병무관.
Objective To study the correlation between the renin-angiotensin-aldosterone system angiotensinogen (AGT) gene M235T,angiotensin Ⅱ type 1 receptor (AGTR1) gene Al166C,aldosterone synthase (CYP11B2) gene -344C/T polymorphisms and large-artery atherosclerotic (LAA) stroke in a southern Chinese Han population.Methods Polymerase chain reaction and gene sequencing technology were used for the genotyping in patients with LAA and normal controls with AGT gene M235T,AGTR1 gene A1166C,and CYP11B2 gene - 344C/T polymorphisms in a southern Chinese Han population,and to determine the correlation between the 3 gene polymorphisms and LAA by binary logistic regression analysis.Results A total of 107 patients with LAA and 142 healthy controls were included in the study.The frequencies of the AGT gene 253TT genotype (66.36% vs.50.70%,x2 =6.122,P =0.047) and T allele (79.44% vs.70.07% %,x2 =5.581,P =0.018) in the LAA group were significantly higher than those in the control group.The frequencies of the AGTR1 gene 1166CC genotype (0% vs.0%,x2 =1.494,P =0.222) and C allele (7.48% vs.4.93%,x2 =1.399,P =0.237) in the LAA group were no significantly differences with those in the control group.The frequencies of the CYP11B2 gene - 344CC genotype (9.35% vs.4.23%,x2 =3.603,P =0.165) and C allele (27.10% vs.26.06%,x2 =0.069,P =0.793) in the LAA group were no significant differences with those in the control group.Binary logistic regression analysis showed that there was no significant correlation between the three gene polymorphisms and the simple LAA diseases.The frequencies of AGT gene 235TT genotype (68.00% vs.41.90%,x2 =12.446,P =0.002) and T allele (79.33% vs.64.76%,x2 =8.993,P =0.003) in the LAA patients complicated with hypertension were significantly higher than those in the normotensive control group.Logistic regression analysis showed that the odds ratio (OR) exposed to TT genotype was 2.153 (95% confidence interval [CI] 0.789-5.872).The OR of T allele was 2.089 (95% CI 1.285-3.396).Conclusions The AGT gene M235T polymorphism is not associated with the simple LAA in the southern Chinese Han population,but it may be associated with the risk of LAA complicated with hypertension;CYP11B2 gene -344C/T polymorphism and AGTR1 gene A1166C polymorphism are not associated with the onset of LAA in the southern Chinese Han population.