中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
7期
446-449
,共4页
黄鹤卿%穆荣%刘霞%王世瑶%李忆农%栗占国
黃鶴卿%穆榮%劉霞%王世瑤%李憶農%慄佔國
황학경%목영%류하%왕세요%리억농%률점국
关节炎,类风湿%白细胞介素类%自身抗体
關節炎,類風濕%白細胞介素類%自身抗體
관절염,류풍습%백세포개소류%자신항체
Arthritis,rheumatoid%Interleukins%Autoantibodies
目的 通过检测早期类风湿关节炎(RA)患者血清中的白细胞介素(IL)-33水平,分析其与早期RA之间的相关性.方法 收集病程<1年的早期RA患者100例,骨关节炎(OA)患者40例以及健康对照者70名.采用双抗体夹心酶联免疫吸附试验(ELISA)测定血清中的IL-33水平,并分析血清IL-33水平与RA各临床和实验室指标的相关性.计量资料的比较采用Kruskal-Wallis检验和(或)Mann-Whitney U检验,计数资料比较采用X2检验,相关性分析采用Spearman相关分析.结果 RA患者血清IL-33水平为(282±871)pg/ml,显著高于健康对照组[(7±38)pg/ml,P(0.01)和OA患者[(8±35)pg/ml,P<0.01].血清IL-33水平与类风湿因子(RF)、隐性类风湿因子IgG(HRF-IgG)、抗环瓜氨酸肽(CCP)抗体、抗突变型瓜氨酸化波形蛋白(MCV)抗体呈正相关(r分别为:0.312,0.277,0.213,0.302,P<0.01或P<0.05). IL-33阳性组患者的RF阳性率、HRF-IgG阳性率、抗CCP抗体阳性率、抗MCV抗体阳性率(86%、31%、86%、94%)较IL-33阴性组患者(54%、11%、42%、72%)显著升高(P均<0.05). 结论 IL-33在RA患者血清中显著升高,并与多种自身抗体(包括RF、抗CCP抗体、抗MCV抗体和HRF-IgG)显著相关,可能是RA预后不良的因素之一.
目的 通過檢測早期類風濕關節炎(RA)患者血清中的白細胞介素(IL)-33水平,分析其與早期RA之間的相關性.方法 收集病程<1年的早期RA患者100例,骨關節炎(OA)患者40例以及健康對照者70名.採用雙抗體夾心酶聯免疫吸附試驗(ELISA)測定血清中的IL-33水平,併分析血清IL-33水平與RA各臨床和實驗室指標的相關性.計量資料的比較採用Kruskal-Wallis檢驗和(或)Mann-Whitney U檢驗,計數資料比較採用X2檢驗,相關性分析採用Spearman相關分析.結果 RA患者血清IL-33水平為(282±871)pg/ml,顯著高于健康對照組[(7±38)pg/ml,P(0.01)和OA患者[(8±35)pg/ml,P<0.01].血清IL-33水平與類風濕因子(RF)、隱性類風濕因子IgG(HRF-IgG)、抗環瓜氨痠肽(CCP)抗體、抗突變型瓜氨痠化波形蛋白(MCV)抗體呈正相關(r分彆為:0.312,0.277,0.213,0.302,P<0.01或P<0.05). IL-33暘性組患者的RF暘性率、HRF-IgG暘性率、抗CCP抗體暘性率、抗MCV抗體暘性率(86%、31%、86%、94%)較IL-33陰性組患者(54%、11%、42%、72%)顯著升高(P均<0.05). 結論 IL-33在RA患者血清中顯著升高,併與多種自身抗體(包括RF、抗CCP抗體、抗MCV抗體和HRF-IgG)顯著相關,可能是RA預後不良的因素之一.
목적 통과검측조기류풍습관절염(RA)환자혈청중적백세포개소(IL)-33수평,분석기여조기RA지간적상관성.방법 수집병정<1년적조기RA환자100례,골관절염(OA)환자40례이급건강대조자70명.채용쌍항체협심매련면역흡부시험(ELISA)측정혈청중적IL-33수평,병분석혈청IL-33수평여RA각림상화실험실지표적상관성.계량자료적비교채용Kruskal-Wallis검험화(혹)Mann-Whitney U검험,계수자료비교채용X2검험,상관성분석채용Spearman상관분석.결과 RA환자혈청IL-33수평위(282±871)pg/ml,현저고우건강대조조[(7±38)pg/ml,P(0.01)화OA환자[(8±35)pg/ml,P<0.01].혈청IL-33수평여류풍습인자(RF)、은성류풍습인자IgG(HRF-IgG)、항배과안산태(CCP)항체、항돌변형과안산화파형단백(MCV)항체정정상관(r분별위:0.312,0.277,0.213,0.302,P<0.01혹P<0.05). IL-33양성조환자적RF양성솔、HRF-IgG양성솔、항CCP항체양성솔、항MCV항체양성솔(86%、31%、86%、94%)교IL-33음성조환자(54%、11%、42%、72%)현저승고(P균<0.05). 결론 IL-33재RA환자혈청중현저승고,병여다충자신항체(포괄RF、항CCP항체、항MCV항체화HRF-IgG)현저상관,가능시RA예후불량적인소지일.
Objective To analyze the correlation between serum interleukin (IL)-33 and early rheumatoid arthritis (RA) by testing the serum IL-33 of patients with RA.Methods One hundred patients with early RA whose disease duration was less than 1 year were selected,40 patients with osteoarthritis (OA) and 70 healthy controis were selected.The serum IL-33 levels of the three groups were detected by enzyme-linked immunosorbent assay (ELISA).The correlation between serum IL-33 levels and clinical features of patients with RA were analyzed.Kruskal-Wallis test,Mann-Whitney U test,X2 test and Spearman correlation analysis were used for statistical analysis. Results The level of serum IL-33 in RA patients [(282±871)pg/ml ] was significantlv higher than that of the healthy controls [(7±38) pg/ml,P<O.01 ] and OA patients [(8±35)pg/ml,P<0.01].The levels of serum IL-33 in RA patients were related with rheumatoid factor (RF),occult rheumatoid factor immunoglobulin G (HRF-IgG),anti-cyclic citrullinated peptide antibodies (anti-CCP),anti-mutated citrullinated vimentin antibodies (anti-MCV).The positive rate of RF,HRF-IgG,anti-CCP and anti-MCV in IL-33 positive group (86%,31%,86%,94%) was significantly higher than that of the IL-33 negative group (54%,11%,42%,72%)(P<0.05).Conclusion The level of serum IL-33 is significantly elevated in RA and it is significanfly associated with some of the auto-antibodies (including RF,anti-CCP antibodies,anti-MCV antibodies and HRF-IgG).Thus,IL-33 may be a factor for unfavorable prognosis to RA patients.
