中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
7期
917-919
,共3页
鼠李科%植物提取物/药理学%乙醇/药理学%肝细胞/药物作用/代谢%脂肪肝,酒精性/代谢/遗传学%解偶联蛋白2/代谢/遗传学
鼠李科%植物提取物/藥理學%乙醇/藥理學%肝細胞/藥物作用/代謝%脂肪肝,酒精性/代謝/遺傳學%解偶聯蛋白2/代謝/遺傳學
서리과%식물제취물/약이학%을순/약이학%간세포/약물작용/대사%지방간,주정성/대사/유전학%해우련단백2/대사/유전학
Rhamnaceae%Plant extracts/pharmacology%Ethanol/pharmacology%Hepatocytes/drug effects/metabolism%Fatty liver,alcoholic/metabolism/genetics%Uncoupling protein 2/metabolism/genetics
目的 通过建立乙醇体外诱导的L-02肝细胞脂肪肝模型,探讨不同剂量的乙醇对肝脏脂肪变L-02细胞解偶联蛋白(Uncoupling protin 2,UCP2)表达水平的关系,观察枳棋子水提取液对其表达的影响.方法 建立肝细胞脂肪变模型,选最适合浓度枳棋子提取液进行干预,采用半定量RT-PCR法测各组UCP2 mRNA表达量.结果 各浓度脂肪变性模型中UCP2 mRNA表达均有下降,经枳棋子水提取液干预后UCP2 mRNA表达均有明显升高(0.6％乙醇组治疗后值由0.4859升至0.7442).结论 乙醇可降低L-02细胞UCP2 mRNA的表达,UCP2 mRNA的低表达可能与L-02细胞AFLD发生有关.枳棋子可使L-02细胞UCP2 mRNA的表达上调,并推测UCP2 mRNA的表达上调是枳棋子减轻酒精诱导肝细胞脂肪变性的机制之一.
目的 通過建立乙醇體外誘導的L-02肝細胞脂肪肝模型,探討不同劑量的乙醇對肝髒脂肪變L-02細胞解偶聯蛋白(Uncoupling protin 2,UCP2)錶達水平的關繫,觀察枳棋子水提取液對其錶達的影響.方法 建立肝細胞脂肪變模型,選最適閤濃度枳棋子提取液進行榦預,採用半定量RT-PCR法測各組UCP2 mRNA錶達量.結果 各濃度脂肪變性模型中UCP2 mRNA錶達均有下降,經枳棋子水提取液榦預後UCP2 mRNA錶達均有明顯升高(0.6％乙醇組治療後值由0.4859升至0.7442).結論 乙醇可降低L-02細胞UCP2 mRNA的錶達,UCP2 mRNA的低錶達可能與L-02細胞AFLD髮生有關.枳棋子可使L-02細胞UCP2 mRNA的錶達上調,併推測UCP2 mRNA的錶達上調是枳棋子減輕酒精誘導肝細胞脂肪變性的機製之一.
목적 통과건립을순체외유도적L-02간세포지방간모형,탐토불동제량적을순대간장지방변L-02세포해우련단백(Uncoupling protin 2,UCP2)표체수평적관계,관찰지기자수제취액대기표체적영향.방법 건립간세포지방변모형,선최괄합농도지기자제취액진행간예,채용반정량RT-PCR법측각조UCP2 mRNA표체량.결과 각농도지방변성모형중UCP2 mRNA표체균유하강,경지기자수제취액간예후UCP2 mRNA표체균유명현승고(0.6％을순조치료후치유0.4859승지0.7442).결론 을순가강저L-02세포UCP2 mRNA적표체,UCP2 mRNA적저표체가능여L-02세포AFLD발생유관.지기자가사L-02세포UCP2 mRNA적표체상조,병추측UCP2 mRNA적표체상조시지기자감경주정유도간세포지방변성적궤제지일.
Objective To establish the model of alcohol-induced hepatocyte steatosis in L-02 liver cells in vitro,investigate the relationship between cell damage,UCP2 gene expression and ethanol concentrations in L-02 cells of steatosis,and explore the protective effect of Hovenia Dulcis Thunb extracted liquid.Method Hovenia Dulcis Thunb extracted liquid at the most compatible dosage was applied to the L-02 cells of steatosis.Then UCP2 mRNA was detected by the semi-quantitative RT-PCR method.Results In steatosis model groups,UCP2 mRNA expression was downregulated ( P ＜0.05).In the steatosis groups treated with Hovenia Dulcis Thunb extracted liquid,UCP2 mRNA level was significantly increased compared to steatosis group ( its expression level increased from 0.4859 to 0.7442 after treatment of 0.6％ ethanol,P＜ 0.05).Conclusions Ethanol can down-regulate the UCP2 mRNA expression of L-02 cells in a certain period.The low expression of UCP2 mRNA is probably relevant to the occurrence of alcoholic fatty liver disease.Hovenia Dulcis Thunb can prevent from the cell toxicity of ethanol and decrease the degree of steatosis.Hovenia Dulcis Thunb extracted liquid can increase the expression level of UCP2 mRNA in the L-02 cells.
