CAJ | 학술논문

目的探讨丹红注射液对家兔颈动脉经皮腔内血管成形(PTA)术后内膜增生和碱性成纤维细胞生长因子(bFGF)及组织因子(TF)表达的影响.方法 50只新西兰大白兔按随机数字表法分为5组:假手术组、模型组和低、中、高剂量丹红注射液治疗组,每组10只;每组再按检测时间点分为术后7 d、28 d 2个亚组,每亚组各5只.假手术组仅结扎颈外动脉,后四组采用球囊损伤颈动脉法制备家兔颈动脉PTA术后狭窄模型.各治疗组于球囊损伤术前第1天开始分别静脉注射丹红注射液1 mL/(kg·d)、2 mL/(kg·d)、4 mL/(kg·d),连续7 d或14 d.假手术组及模型组给予静脉注射生理盐水2 mL/(kg·d).术后7、28 d时分别取模型靶血管进行组织学观察,免疫组化染色检测bFGF和TF的表达,图像分析系统进行半定量分析.结果术后7 d时模型组新生内膜面积、新生内膜面积/中层面积(I/M)与其他组比较差异无统计学意义(P>0.05);术后28 d时模型组新生内膜面积、I/M比值较假手术组明显增加,而中、高剂量丹红注射液治疗组较模型组均明显降低,差异有统计学意义(P<0.05).与模型组相比,中、高剂量丹红注射液治疗组血管壁bFGF和TF表达量均明显降低,差异有统计学意义(P<0.05),而低剂量丹红注射液治疗组差异无统计学意义(P>0.05).结论中、高剂量组丹红注射液可抑制家兔颈动脉PTA术后内膜增生,其机制可能是通过下调动脉壁bFGF和TF的表达来实现的.
목적 탐토단홍주사액대가토경동맥경피강내혈관성형(PTA)술후내막증생화감성성섬유세포생장인자(bFGF)급조직인자(TF)표체적영향.방법 50지신서란대백토안수궤수자표법분위5조:가수술조、모형조화저、중、고제량단홍주사액치료조,매조10지;매조재안검측시간점분위술후7 d、28 d 2개아조,매아조각5지.가수술조부결찰경외동맥,후사조채용구낭손상경동맥법제비가토경동맥PTA술후협착모형.각치료조우구낭손상술전제1천개시분별정맥주사단홍주사액1 mL/(kg·d)、2 mL/(kg·d)、4 mL/(kg·d),련속7 d혹14 d.가수술조급모형조급여정맥주사생리염수2 mL/(kg·d).술후7、28 d시분별취모형파혈관진행조직학관찰,면역조화염색검측bFGF화TF적표체,도상분석계통진행반정량분석.결과 술후7 d시모형조신생내막면적、신생내막면적/중층면적(I/M)여기타조비교차이무통계학의의(P>0.05);술후28 d시모형조신생내막면적、I/M비치교가수술조명현증가,이중、고제량단홍주사액치료조교모형조균명현강저,차이유통계학의의(P<0.05).여모형조상비,중、고제량단홍주사액치료조혈관벽bFGF화TF표체량균명현강저,차이유통계학의의(P<0.05),이저제량단홍주사액치료조차이무통계학의의(P>0.05).결론 중、고제량조단홍주사액가억제가토경동맥PTA술후내막증생,기궤제가능시통과하조동맥벽bFGF화TF적표체래실현적.
Objecfive To study the effect of Danhong injection(DI)on intimal hyperplasia and expressions of basic fibroblast growth factor(bFGF)and tissue factor(TF)in carotid arteries of rabbits after percutaneous transluminal angioplasty(PTA). Methods Fifty New Zealand White rabbits were randomly divided into 5 groups (n=10),namely sham-operated group,model group,and DI treatment groups(low-,medium-and high-dose DI treatment groups).The animals in the sham-operated group were only given external carotid artery ligation.The rabbit models of carotid stenosis in the later 4 groups were established by ballon-injury.Before the carotid balloon injury,the animals in the DI treatment groups were given DI at doses of 1 mL/kg·d-1,2mL/kg·d-1 and 4 mL/kg·d-1,respectively,for 7 or 14 d via intravenous injection;the animals in the sham-operated and model groups were given saline solution(2mL/kg·d-1)instead.The animals were sacrificed and their common carotid arteries were removed on the 7th and 28th d of surgery;morphology changes of the arterial wall with hematoxylin eosin staining were observed by optical microscope,and the expressions ofbFGF and TF of the vascular wall were detected through immunohistochemical techniques;with the help of an image analysis system,semiquantitative analysis was performed.Results On the 7th d of surgery,the intimal area and intima area/media area (I/M)ratio in the model group showed no significant differences as compared with those in the other 4groups(P>0.05).On the 28th d of surgery,the intimai area and I/M ratio in the model group were increased markedly as compared with those in the sham-operated group (P<0.05), and the intimal area and I/M ratio in the medium and high dose DI treatment groups were significantly reduced as compared with those in the in the model group (P<0.05). The expressions of TF and bFGF in the neointima of carotid arteries of rabbits treated with medium and high dose of DI were significantly weaker than those of the model group (P<0.05), and those of rabbits treated with low dose of DI had no obvious differences as compared with those of the model group (P>0.05). Conclusion Medium and high dose of DI can inhibit the intimal hyperplasia resulting from percutaneous transluminal angioplasty, might through the inhibition of expressions of bFGF and TF in the neointima of the arterial wall.