CAJ | 학술논문

目的了解宁夏地区1984-2011年小肠结肠炎耶尔森菌菌型变迁情况及遗传进化特征.方法对1984-2011年由腹泻患者、猪、鼠类、羊和犬标本中分离的296株小肠结肠炎耶尔森菌进行血清分型、生物分型及黏附侵袭位点基因( ail)、肠毒素基因A(ystA)、ystB、黏附素基因A(yadA)、毒力因子基因F(virF)等毒力基因检测,同时对O:3和O:9血清型菌株进行PFGE分型,并进行聚类分析.结果 296株小肠结肠炎耶尔森菌中,猪是最主要的宿主,占菌株分离总数的65.20％(193/296),其次是鼠类,占32.43％(96/296).血清型和生物型在不同时期均具有各自的优势型别,1984-1985年共分离2株O:3和3株O:9血清型菌株,均为生物3型,菌株较少,优势型别不明显；1997-1999年分离到177株O:9血清型菌株,为这一时期的优势型别,生物2型分离株有178株；2007-2011年分离到54株O:3血清型菌株,为此时期的优势型别,其次为O:5血清型,分离到26株,生物型以1A和3型为主,分别为44、59株.PCR检测将分离到的248株小肠结肠炎耶尔森菌毒力携带情况分为4型,其中致病性菌株均为Ⅰ型(ail+、ystA+、ystB -、yadA+、virF+)；采用PFGE分型将O:3血清型菌株分为12个型别,K6GN11 C30021、K6GN11 C30012为主要带型,占所有分型菌株的63.64％(42/66)；O:9血清型分为14个型别,K6GN11C90010、K6GN11C90008、K6GN11C30018、K6GN11 C90003为主要型别,占所有分型菌株的89.01％ (162/182).结论宁夏地区小肠结肠炎耶尔森菌分离菌株的血清型在不同时期具有不同的优势菌型,且生物型随着血清型的改变亦发生动态变化.分离自不同年代的致病性小肠结肠炎耶尔森菌的变异较大.
목적 료해저하지구1984-2011년소장결장염야이삼균균형변천정황급유전진화특정.방법 대1984-2011년유복사환자、저、서류、양화견표본중분리적296주소장결장염야이삼균진행혈청분형、생물분형급점부침습위점기인( ail)、장독소기인A(ystA)、ystB、점부소기인A(yadA)、독력인자기인F(virF)등독력기인검측,동시대O:3화O:9혈청형균주진행PFGE분형,병진행취류분석.결과 296주소장결장염야이삼균중,저시최주요적숙주,점균주분리총수적65.20％(193/296),기차시서류,점32.43％(96/296).혈청형화생물형재불동시기균구유각자적우세형별,1984-1985년공분리2주O:3화3주O:9혈청형균주,균위생물3형,균주교소,우세형별불명현；1997-1999년분리도177주O:9혈청형균주,위저일시기적우세형별,생물2형분리주유178주；2007-2011년분리도54주O:3혈청형균주,위차시기적우세형별,기차위O:5혈청형,분리도26주,생물형이1A화3형위주,분별위44、59주.PCR검측장분리도적248주소장결장염야이삼균독력휴대정황분위4형,기중치병성균주균위Ⅰ형(ail+、ystA+、ystB -、yadA+、virF+)；채용PFGE분형장O:3혈청형균주분위12개형별,K6GN11 C30021、K6GN11 C30012위주요대형,점소유분형균주적63.64％(42/66)；O:9혈청형분위14개형별,K6GN11C90010、K6GN11C90008、K6GN11C30018、K6GN11 C90003위주요형별,점소유분형균주적89.01％ (162/182).결론 저하지구소장결장염야이삼균분리균주적혈청형재불동시기구유불동적우세균형,차생물형수착혈청형적개변역발생동태변화.분리자불동년대적치병성소장결장염야이삼균적변이교대.
Objective To analyze the genetic evolution and bacterial type changes of Yersinia enterocolitica in the Ningxia area between year 1984 and 2011.Methods A total of 296 strains of Yersinia enterocolitica was collected from diarrhea patients,pig,rodents,sheep and dogs between year 1984 and 2011.The serotype,biotype,ail,ystA,ystB,yadA,virF and other toxic genes were detected.The PFGE subtypes of serotype O:3 and O:9 strains and the cluster features were analyzed.Results Out of 296 Yersinia enterocolitica strains,pig was the main host,accounting for 65.20％ (193/296),followed by rodents,accounting for 32.43％ (96/296).Serotype and biotype had their own respective dominant types in different periods.During 1984 and 1985,2 strains of serotype O:3 and 3 strains of serotype O:9 were isolated,all belonged to biotype 3.Because of lack of strains,there were no obvious dominant types found.Between 1997 and 1999,177 strains of serotype O:9 Yersinia enterocolitica were isolated as the dominant strain ; and there were 178 strains of biotype 2 Yersinia enterocolitica were found.During 2007 and 2011,54 strains of serotype O:3 Yersinia enterocolitica were isolated as dominant strain; followed by 26 strains of serotype O:5.There were separately 44 and 59 strains of biotype 1A and biotype 3.The PCR test divided the 248 strains into 4 types,including pathogenic strains as type Ⅰ ( ail +,ystA+,ystB+,yadA +,virF+ ).The PFGE divided the serotype O:3 into 12 types,in which K6GN11 C30021 and K6GN11 C30012 were the dominant types,accounting for 63.64％ (42/66).The serotype O:9 were divided into 14 types,in which K6GN11C90010,K6GN11C90008,K6GN11C30018 and K6GN11 C90003 were the dominant types,accounting for 89.01％ (162/182).Conclusion The different serotypes of isolated strains in Ningxia district showed different dominant bacteria in different periods; while the biotypes also changed with serotypes.The Yersinia enterocolitica isolated from different years showed great variation.