CAJ | 학술논문

目的探讨缺氧诱导因子(HIF)-1α,血管内皮素(ET)-1在胃肠道间质瘤(GISTs)中的表达及其在预后判断中的价值.方法应用免疫组织化学SP法对76例GISTs检测,观察HIF-1α、ET-1在GISTs中的表达情况.结果 HIF-1α、ET-1在GISTs中的阳性表达率分别为73.68%(56/76)、65.79(50/76),两者的表达呈正相关(P<0.05),其与胃肠道间质瘤的组织学分级、肿瘤直径、浸润转移和核分裂数有关(P<0.05),而与患者的年龄、性别、肿瘤原发部位无关(P>0.05);HIF-1α、ET-1在极低和低危险,中度危险和高危险及直径<2 cm和直径>5 cm组间差异有统计学意义(P<0.05),阳性表达率随恶性程度的增加和肿瘤直径的增大而增加(P<0.05),在有浸润转移和核分裂数≥5/50HP组中的表达明显高于无浸润转移和核分裂数<5/50HP组中的表达(P<0.05).结论 HIF-1α、ET-1在GISTs中的表达有联合倾向,可能提示肿瘤的恶性程度及其预后,并可作为判断预后的有用标志物.
목적 탐토결양유도인자(HIF)-1α,혈관내피소(ET)-1재위장도간질류(GISTs)중적표체급기재예후판단중적개치.방법 응용면역조직화학SP법대76례GISTs검측,관찰HIF-1α、ET-1재GISTs중적표체정황.결과 HIF-1α、ET-1재GISTs중적양성표체솔분별위73.68%(56/76)、65.79(50/76),량자적표체정정상관(P<0.05),기여위장도간질류적조직학분급、종류직경、침윤전이화핵분렬수유관(P<0.05),이여환자적년령、성별、종류원발부위무관(P>0.05);HIF-1α、ET-1재겁저화저위험,중도위험화고위험급직경<2 cm화직경>5 cm조간차이유통계학의의(P<0.05),양성표체솔수악성정도적증가화종류직경적증대이증가(P<0.05),재유침윤전이화핵분렬수≥5/50HP조중적표체명현고우무침윤전이화핵분렬수<5/50HP조중적표체(P<0.05).결론 HIF-1α、ET-1재GISTs중적표체유연합경향,가능제시종류적악성정도급기예후,병가작위판단예후적유용표지물.
Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.