中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
1期
102-104
,共3页
孙忠东%夏家红%董念国%杨辰垣
孫忠東%夏傢紅%董唸國%楊辰垣
손충동%하가홍%동념국%양신원
酸性复灌液%未成熟心肌%心肌保护
痠性複灌液%未成熟心肌%心肌保護
산성복관액%미성숙심기%심기보호
Acidic reperfusation%Immature myocardium%Cardioprotection
目的 观察不同pH值HEPES-KH复灌液对离体未成熟心肌的影响.方法 建立Langendorff离体灌注模型,分为2组:缺血/再灌(I/R,n=8),用pH 7.4 HEPES-KH液灌流20 min,缺血60min,恢复灌注30min;酸性灌注组(E,n=8),用pH7.4HEPES-KH液灌流20min,缺血60min后,应用pH 6.8、7.1和7.4 HEPES-KH液顺次灌注5、5、20 min.以血流动力学指标、生化指标作为观察指标.结果 E组与I/R组比较,左心室功能恢复、三磷酸腺苷含量(ATP)(0.93±0.12比0.56±0.04,P＜0.01)、超氧化物歧化酶活性(183.47±9.72比120.17±6.21,P＜0.01)、心肌线粒体Ca2+-ATP酶活性(16.74±1.42比6.78±0.64,P＜0.01)和心肌线粒体合成ATP的能力(105.37±9.51比50.83±4.75,P＜0.01)明显增强,在心肌含水量(74.56±1.68比86.20±2.33,P＜0.01)、丙二醛含量(1. 97±0.17比2.88±0.32,P＜0.01)、肌酸激酶(64.56±4.69比88.48±5.86,P＜0.01)和乳酸脱氢酶漏出率漏出率(96.41±6.57比128.42±9.80,P＜0.01)、心肌细胞内Ca2+含量(2.25±0.28比4.48±0.74,P＜0.01)和心肌线粒体Ca2+含量(36.10±4.05比68.29±6.90,P＜0.01)明显减少.结论 复灌初期应用梯度酸性复灌液对离体未成熟心肌具有明显保护作用.
目的 觀察不同pH值HEPES-KH複灌液對離體未成熟心肌的影響.方法 建立Langendorff離體灌註模型,分為2組:缺血/再灌(I/R,n=8),用pH 7.4 HEPES-KH液灌流20 min,缺血60min,恢複灌註30min;痠性灌註組(E,n=8),用pH7.4HEPES-KH液灌流20min,缺血60min後,應用pH 6.8、7.1和7.4 HEPES-KH液順次灌註5、5、20 min.以血流動力學指標、生化指標作為觀察指標.結果 E組與I/R組比較,左心室功能恢複、三燐痠腺苷含量(ATP)(0.93±0.12比0.56±0.04,P＜0.01)、超氧化物歧化酶活性(183.47±9.72比120.17±6.21,P＜0.01)、心肌線粒體Ca2+-ATP酶活性(16.74±1.42比6.78±0.64,P＜0.01)和心肌線粒體閤成ATP的能力(105.37±9.51比50.83±4.75,P＜0.01)明顯增彊,在心肌含水量(74.56±1.68比86.20±2.33,P＜0.01)、丙二醛含量(1. 97±0.17比2.88±0.32,P＜0.01)、肌痠激酶(64.56±4.69比88.48±5.86,P＜0.01)和乳痠脫氫酶漏齣率漏齣率(96.41±6.57比128.42±9.80,P＜0.01)、心肌細胞內Ca2+含量(2.25±0.28比4.48±0.74,P＜0.01)和心肌線粒體Ca2+含量(36.10±4.05比68.29±6.90,P＜0.01)明顯減少.結論 複灌初期應用梯度痠性複灌液對離體未成熟心肌具有明顯保護作用.
목적 관찰불동pH치HEPES-KH복관액대리체미성숙심기적영향.방법 건립Langendorff리체관주모형,분위2조:결혈/재관(I/R,n=8),용pH 7.4 HEPES-KH액관류20 min,결혈60min,회복관주30min;산성관주조(E,n=8),용pH7.4HEPES-KH액관류20min,결혈60min후,응용pH 6.8、7.1화7.4 HEPES-KH액순차관주5、5、20 min.이혈류동역학지표、생화지표작위관찰지표.결과 E조여I/R조비교,좌심실공능회복、삼린산선감함량(ATP)(0.93±0.12비0.56±0.04,P＜0.01)、초양화물기화매활성(183.47±9.72비120.17±6.21,P＜0.01)、심기선립체Ca2+-ATP매활성(16.74±1.42비6.78±0.64,P＜0.01)화심기선립체합성ATP적능력(105.37±9.51비50.83±4.75,P＜0.01)명현증강,재심기함수량(74.56±1.68비86.20±2.33,P＜0.01)、병이철함량(1. 97±0.17비2.88±0.32,P＜0.01)、기산격매(64.56±4.69비88.48±5.86,P＜0.01)화유산탈경매루출솔루출솔(96.41±6.57비128.42±9.80,P＜0.01)、심기세포내Ca2+함량(2.25±0.28비4.48±0.74,P＜0.01)화심기선립체Ca2+함량(36.10±4.05비68.29±6.90,P＜0.01)명현감소.결론 복관초기응용제도산성복관액대리체미성숙심기구유명현보호작용.
Objective To study the protective effects of different pH HEPES-KH reperfusate solutions on immature myodium. Methods The isolated Langendorff perfused model from immature rabbit hearts was established. The rabbits in ischemia/reperfusion (I/R) group were perfused with pH7.4HEPES-KH solutions preischemia and postischemia. In experimental (E) group, pH 6. 8, pH 7. 1 and pH 7. 4 HEPES-KH solutions were perfused for 5, 5 and 20 min postischemia, respectively. The hemodynamics and biochemistry were tested. Results The left ventricular function was significantly improved, adenosine triphosphate (ATP) content (0. 93 ±0. 12 vs 0. 56 ±0. 04,P ＜0. 01 ), superoxide dismutase activity ( 183.47 ±9. 72 vs 120. 17 ± 6. 21, P ＜ 0. 01 ), Ca2+ -ATPase activity of mitothondia ( 16. 74 ± 1.42 vs 6. 78 ± 0. 64, P ＜ 0. 01 ), ATP activity of mitochondria ( 105.37 ± 9. 51 vs 50. 83 ± 4. 75, P ＜ 0. 01 ) were significantly increased in E group as compared with those in I/R group. Myocardial water content (74. 56 ± 1.68 vs 86. 20 ±2. 33 ,P ＜0. 01 ), malondialdehyde content ( 1.9710. 17 vs 2. 88 ±0. 32,P ＜0. 01 ), dehydrogenase (64. 56 ± 4. 69 vs 88. 48 ± 5. 86, P ＜ 0. 01 ) and creatine kinase leakage (96. 41 ±6.57 vs 128.42 ±9.80,P＜0.01), myocardial cell Ca2+ content (2.25 ±0.28 vs 4.48 ±0.74,P＜0.01) and mitochondrial Ca2+ content (36. 10 ±4.05 vs 68.29 ±6.90,P＜0.01) in E group were reduced as compared with those in I/R group. Conclusion pH paradox might be one of important mechnisms for immature myocardial I/R injury, and acidic perfusate, at the beginning of reperfusion, might attenuate pH paradox and ameliorate functional recovery on isolated immature rabbit hearts.
