CAJ | 학술논문

采用16S rDNA特征序列PCR-DGGE法,分析了不同饵料饲养的暗纹东方鲀的表皮、肠道和河鲀毒素累积组织(肝和卵巢)中可培养细菌的群落组成.根据分子系统发育分析,共鉴定出45种可培养细菌,在这些细菌中,以变形细菌的gamma亚群占多数,其它分别隶属于低GC含量革兰氏阳性菌和高GC含量革兰氏阳性菌.摄食不同饵料的暗纹东方鲀,其肠道、表皮或河鲀毒素累积组织中的细菌菌落组成是不同的,但不管是摄食人工配合饲料或天然饵料,在暗纹东方鲀的肠道、表皮或河鲀毒素累积组织中,均发现已有报道的可产河鲀毒素的细菌类群,表明饵料来源对暗纹东方鲀的河鲀毒素产生不是必需的.
채용16S rDNA특정서렬PCR-DGGE법,분석료불동이료사양적암문동방돈적표피、장도화하돈독소루적조직(간화란소)중가배양세균적군락조성.근거분자계통발육분석,공감정출45충가배양세균,재저사세균중,이변형세균적gamma아군점다수,기타분별대속우저GC함량혁란씨양성균화고GC함량혁란씨양성균.섭식불동이료적암문동방돈,기장도、표피혹하돈독소루적조직중적세균균락조성시불동적,단불관시섭식인공배합사료혹천연이료,재암문동방돈적장도、표피혹하돈독소루적조직중,균발현이유보도적가산하돈독소적세균류군,표명이료래원대암문동방돈적하돈독소산생불시필수적.
Using 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) technology, we investigated the composition of culturable bacterial communities in the skin, intestines, and TTX-accumulating tissues (ovary, liver) of the obscure striped puffer fish Takifugu obscurus fed natural or artificial diets. A total of 45 species of different culturable bacteria were isolated and classified according to phylogenetic analysis. Of these species, those belonging to the class γ-Proteobacteria dominated the microbial community in the puffer fish, while others belonged to the group of low G+C Gram positive bacteria, and the group of high G+C Gram positive bacteria. Large differences in the bacterial assemblage isolated from the intestines, TTX-producing organs and skins of fish fed natural and artificial diets were observed. No matter fed with natural or artificial diets, the tissue of intestine, TTX-accumulating organ, or skin, was found to contain bacteria from TTX-producing genera reported to-date. Puffer fish restricted to artificial diets from birth still had large assemblages of TTX-producing bacteria, indicating that food chain accumulation of TTX contributes little to the toxicity of these fish.