CAJ | 학술논문

[目的]为杂种小麦加工品质预测和优质小麦亲本组配提供依据.[方法]以杂交小麦母本晋麦47和父本丰优1号及其F2籽粒为材料,采用SDS-PAGE电泳法对小麦籽粒中的高分子量麦谷蛋白亚基(HMW-GS)的分离及表达进行分析.[结果]F2籽粒中共出现21种HMW-GS带型组合,其中回归母本的带型(N、7+9、3+12)占69.10%,回归父本的带型(1、17+18、5+10)占1.04%,其余29.86%为双亲杂合带型或缺失带型;HMW-GS在F2籽粒中按一定规律进行分离,Glu-Dlb,Glu-Dld出现基因重组(重组率5.90%),17+18和7+9亚基同时在Glu-B1位点表达,而Glu-Blc在该位点出现表达空位(空位率5.20%).[结论]明确了HMW-GS在小麦F2籽粒中的表达及分离规律.
[목적]위잡충소맥가공품질예측화우질소맥친본조배제공의거.[방법]이잡교소맥모본진맥47화부본봉우1호급기F2자립위재료,채용SDS-PAGE전영법대소맥자립중적고분자량맥곡단백아기(HMW-GS)적분리급표체진행분석.[결과]F2자립중공출현21충HMW-GS대형조합,기중회귀모본적대형(N、7+9、3+12)점69.10%,회귀부본적대형(1、17+18、5+10)점1.04%,기여29.86%위쌍친잡합대형혹결실대형;HMW-GS재F2자립중안일정규률진행분리,Glu-Dlb,Glu-Dld출현기인중조(중조솔5.90%),17+18화7+9아기동시재Glu-B1위점표체,이Glu-Blc재해위점출현표체공위(공위솔5.20%).[결론]명학료HMW-GS재소맥F2자립중적표체급분리규률.
[Objective] The aim was to provide the basis for forecasting processing quality of hybrid wheat and combining parents in high quality wheat breeding process.[Method] With grains of female parent Jinmai 47, male parent Fengyou 1 and F2 of hybrid wheat as the materials, the isolation and expression of high molecular weight glutenin subunits (HMW-GS) in grains were analyzed by SDS-PAGE electrophoresis.[Result] 21 kinds of band phenotypes of HMW-GS appeared in F2 grains.Among which band phenotypes that regressed to female parent (N, 7+9, 3+12) was 69.10%, and that regressed to male parent (1、17+18、5+10) was 1.04%, the remained 29.86% band phenotypes were hybrid band phenotypes and deletion band phenotypes..HMW-GS isolated in F2 grains according to a certain inheritance, Glu-Dlb and Glu-Dld was recombined (recombination rate of 5.90%), 17+18 and 7+9 subunits expressed at Glu-B1 site at the same time, while there were no expression of Glu-Blc at this site (vacancy rate of 5.20%).[Conclusion] This study determined the law of expression and isolation of HMW-GS in F2 grain of wheat.