CAJ | 학술논문

试验用大肠杆菌脂多糖(LPS)多次刺激断奶仔猪建立应激模型,研究α-酮戊二酸(AKG)对断奶仔猪的生长性能和血浆激素水平的影响,探讨AKG能否缓解仔猪应激反应.选用24头健康断奶仔猪(杜洛克×长白×大白,体重(7.25±0.23)kg),随机分成3个组(空白对照组、应激对照组和AKG组),每组8个重复,每个重复1头猪.2个对照组饲喂基础日粮+1%淀粉,AKG组饲喂基础日粮+1%AKG,在试验第10天、第12天、第14天和第16天分别在应激对照组和AKG组仔猪的腹膜注射80 μg/kg LPS.试验结果显示:(1)试验第1～9天,未注射LPS时,AKG组平均日增重分别比空白对照组和应激对照组提高30.0%(P<0.05)和31.1%(P<0.05),料重比降低12.4%(P<0.05)和12.8%(P<0.05);试验第10～16天(注射LPS),应激对照组与空白对照组相比平均日增重降低28.8%(P<0.05),料重比升高16.8%(P<0.05),但AKG组的平均日增重和料重比与空白对照组相比无显著性差异(P>0.05);(2)与空白对照组相比,试验第10天,首次LPS刺激后,应激对照组的血浆胰岛素样生长因子(IGF-I)水平降低22.7%(P<0.05),而AKG组无显著变化(P>0.05);试验第16天,多次LPS刺激后,与空白对照组比较,应激对照组的IGF-I和胰岛素(INS)水平分别降低21.2%(P<0.05)和25.7%(P<0.05),而AKG组无显著变化(P>0.05).结果表明,日粮中添加1%AKG能显著缓解LPS应激对杜×长×大断奶仔猪生长的负影响.
시험용대장간균지다당(LPS)다차자격단내자저건립응격모형,연구α-동무이산(AKG)대단내자저적생장성능화혈장격소수평적영향,탐토AKG능부완해자저응격반응.선용24두건강단내자저(두락극×장백×대백,체중(7.25±0.23)kg),수궤분성3개조(공백대조조、응격대조조화AKG조),매조8개중복,매개중복1두저.2개대조조사위기출일량+1%정분,AKG조사위기출일량+1%AKG,재시험제10천、제12천、제14천화제16천분별재응격대조조화AKG조자저적복막주사80 μg/kg LPS.시험결과현시:(1)시험제1～9천,미주사LPS시,AKG조평균일증중분별비공백대조조화응격대조조제고30.0%(P<0.05)화31.1%(P<0.05),료중비강저12.4%(P<0.05)화12.8%(P<0.05);시험제10～16천(주사LPS),응격대조조여공백대조조상비평균일증중강저28.8%(P<0.05),료중비승고16.8%(P<0.05),단AKG조적평균일증중화료중비여공백대조조상비무현저성차이(P>0.05);(2)여공백대조조상비,시험제10천,수차LPS자격후,응격대조조적혈장이도소양생장인자(IGF-I)수평강저22.7%(P<0.05),이AKG조무현저변화(P>0.05);시험제16천,다차LPS자격후,여공백대조조비교,응격대조조적IGF-I화이도소(INS)수평분별강저21.2%(P<0.05)화25.7%(P<0.05),이AKG조무현저변화(P>0.05).결과표명,일량중첨가1%AKG능현저완해LPS응격대두×장×대단내자저생장적부영향.
Constituting a stress model by repeatedly challenged with lipopolysaccharide (LPS) in weaned pigs, the experiment that was researched on effects of α-ketoglutaric acid (AKG) on growth performance and plasma hormones level was aimed at whether AKG could alleviate the reaction to stress in weaned pigs.Twenty-four healthy crossbred Duroc× Landrace × Largewhite weaned piglets with body weight of (7.25±0.23) kg were randomly allocated to 3 groups (control group, LPS control group and AKG group).Every group included 8 replicates and 1 piglet in per replicate.The control group and LPS control group were fed the basal ration adding with 1% amylum, while AKG group was fed the basal ration adding with 1% AKG.Piglets in LPS control and AKG group were injected intraperitoneally with 80 μg/kg LPS every other day (on day 10, 12, 14 and 16 of experiment).The results showed that: 1) from day 1 to day 9 (without LPS challenge), ADG in AKG group increased by 30.0% (P<0.05) and 31.1% (P<0.05) compared with control group and LPS control group, respec-tively; meanwhile, F/G decreased by 12.4% (P<0.05) and 12.8% (P<0.05); from day 10 to day 16 (with LPS chal-lenge), ADG and F/G in LPS control group decreased by 28.8% (P<0.05) and increased by 16.8% (P<0.05) compared with control group, respectively; while, there was no significant difference between AKG group and control group on ADG and F/G (P>0.05) ; 2) in comparison with control group, insulin-like growth factor-Ⅰ(IGF-Ⅰ) of plasma in LPS control group decreased by 22.7% (P<0.05) after LPS challenged firstly (on day 10 of experiment), but no significant difference was found in AKG group and control group; after repeated challenge with LPS (on day 16 of experiment), IGF-Ⅰ and insu-lin of plasma in LPS group decreased by 21.2% (P<0.05)and 25.7% (P<0.05) ; meanwhile, these indexes had no signifi-cant difference between AKG group and control group (P>0.05).The results indicated that dietary supplementation with 1% AKG could significantly relieve the growth depression of weaned piglets challenged by LPS.