CAJ | 학술논문

目的建立测定血浆中自藜芦醇苷的方法,并对其在Beagle犬体内的药代动力学进行研究.方法采用Hypersil-BDS C18色谱柱;流动相为甲醇-水(35:65),流速1.0 ml/min;紫外检测波长为290 nm,柱温25℃.用非室模型计算药代动力学参数.结果白藜芦醇苷在0.21～21.0μg/ml浓度范围内,其线性良好,r=0.9995(n=5),最低检测浓度约为0.1μg/ml.平均回收率达到90%以上,RSD值低于8.0%.其主要药代动力学参数在3个剂量组的T_(1/2)分别为89.95±19.96 min,152.15±55.82 min,202.36±66.75 min,AUCO-∞分别为300.14±51.33μg·min/ml,751.72±173.97μg·min/ml和1521.12±310.02μg·min/ml.结论用本法测定血浆中自藜芦醇苷,结果满意,白藜芦醇苷在体内符合二室模型.
목적 건립측정혈장중자려호순감적방법,병대기재Beagle견체내적약대동역학진행연구.방법 채용Hypersil-BDS C18색보주;류동상위갑순-수(35:65),류속1.0 ml/min;자외검측파장위290 nm,주온25℃.용비실모형계산약대동역학삼수.결과 백려호순감재0.21～21.0μg/ml농도범위내,기선성량호,r=0.9995(n=5),최저검측농도약위0.1μg/ml.평균회수솔체도90%이상,RSD치저우8.0%.기주요약대동역학삼수재3개제량조적T_(1/2)분별위89.95±19.96 min,152.15±55.82 min,202.36±66.75 min,AUCO-∞분별위300.14±51.33μg·min/ml,751.72±173.97μg·min/ml화1521.12±310.02μg·min/ml.결론 용본법측정혈장중자려호순감,결과만의,백려호순감재체내부합이실모형.
Objective To establish a method for detecting plasma polydatin using high-performance liquid chromatography (HPLC) and investigate the pharmacokinetics of polydatin in Beagle dogs. Methods HPLC-based detection of polydatin was performed on a Hypersil-BDS C18 column with a mobile phase of methanol and water (35=65) at the flow rate of 1.0 ml/min and the detection wavelength of 290 nm. The pharmacokinetic parameters of polydatin were calculated by non-compartment model statistics. Results The standard curve was linear within the range of 0.21-21.0 (Ag/ml (correlation coefficient of 0.9995,n=5). The average recovery was 90% with a relative standard deviation no more than 8.0%. The limit of detection of the method was 0.1 μ/ml. The pharmacokinetic parameters of polydatin at 3 doses were obtained, with T_(1/2) of 89.95±19.96 min,152.15±55.82 min, and 202.36±66.75 min, and AUC0-∞ of 300.14±S1.33 g·min·ml~(-1), 751.72+173.97 g·min·ml~(-1) and 1521.12±310.02 μg· min· ml~(-1), respectively. Conclusion The method we established allows effective detection of polydatin,whose pharmacokinetics conforms to the two-compartment model.