CAJ | 학술논문

以长豇豆高抗锈病材料‘ZN016'和高感品种‘之豇282'为材料,通过基质栽培,研究锈菌胁迫下外源硅对长豇豆叶片细胞质、线粒体和叶绿体抗氧化酶活性与抗氧化物质含量的影响.结果表明:锈菌胁迫下,外源硅可显著提高豇豆细胞质、线粒体和叶绿体中过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和苯丙氨酸解氨酶(PAL)的活性以及线粒体内的超氧化物歧化酶(SOD)活性;同时显著提高细胞质内的多酚含量和感病品种线粒体中抗坏血酸(AsA)的含量;外源硅可显著降低感病品种细胞质和叶绿体中的丙二醛(MDA)含量.推测锈菌胁迫下外源硅可能通过提高豇豆叶片细胞质和叶绿体的抗氧化能力,从而避免活性氧对细胞质膜和叶绿体膜系统的伤害,维持其正常的生理功能.
이장강두고항수병재료‘ZN016'화고감품충‘지강282'위재료,통과기질재배,연구수균협박하외원규대장강두협편세포질、선립체화협록체항양화매활성여항양화물질함량적영향.결과표명:수균협박하,외원규가현저제고강두세포질、선립체화협록체중과양화물매(POD)、항배혈산과양화물매(APX)、과양화경매(CAT)화분병안산해안매(PAL)적활성이급선립체내적초양화물기화매(SOD)활성;동시현저제고세포질내적다분함량화감병품충선립체중항배혈산(AsA)적함량;외원규가현저강저감병품충세포질화협록체중적병이철(MDA)함량.추측수균협박하외원규가능통과제고강두협편세포질화협록체적항양화능력,종이피면활성양대세포질막화협록체막계통적상해,유지기정상적생리공능.
The effects of exogenous silicon (Si) on antioxidant ability of two asparagus bean cultivars, 'ZN016' (highly resistant) and 'Zhijiang 282' (susceptible), were investigated at sub-cellular level (cytoplasm, chloroplast and mitochondria) under rust (Uromyces vignae) stress by substrate culture. The results indicate that application of exogenous Si significantly increased the activities of peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), and phenylalanine ammonia-lyase (PAL) in three sub-cellular fractions, and superoxide dismutase (SOD) activity of mitochondria for both cultivars, and the ascorbate acid (AsA) content in mitochondria of susceptible cultivar under rust stress. Consequently, the content of malonyldialdehyde (MDA) in cytoplasm and chloroplast of susceptible cultivar was significantly reduced by exogenous Si application under rust stress. These results above indicated that enhanced rust resistance of asparagus bean by exogenous Si application could increase their antioxidant capacities, which can quench reactive oxygen species more efficiently and maintain the integrity and biological function of cytoplasmic membrane and chloroplast membrane.