CAJ | 학술논문

在家蚕基因组中检索分析了与果蝇86个胚胎发育相关基因同源的基因.分析结果表明,在果蝇的这86个基因中,有62(72%)个基因在家蚕基因组中存在直向同源关系,其中58(67%)个基因并且在家蚕、果蝇、冈比亚按蚊3种昆虫的基因组间呈现 1:1:1直向同源关系.通过比较分析发现,体节极性决定基因在3种昆虫基因组间最为保守.同果蝇和冈比亚按蚊相比,家蚕中决定背-腹部形成的基因分化最大.进一步利用芯片数据对其中的12个母性基因在家蚕5龄第3天幼虫生殖腺中的表达情况进行分析:有2个基因(orb和nanos)在卵巢中特异表达;4个基因(spire、Ras85D、gd和arrest)在精巢中特异表达;另外的6个基因(exuperantia、vasa、pumilio、mago nashi、nudel和dorsal)在精巢和卵巢中均有表达,但是其中的2个基因(pumilio和nudel)在雌、雄间的表达量存在显著差异.研究结果为家蚕发育调控机制的进一步研究提供了重要线索和数据信息.
재가잠기인조중검색분석료여과승86개배태발육상관기인동원적기인.분석결과표명,재과승적저86개기인중,유62(72%)개기인재가잠기인조중존재직향동원관계,기중58(67%)개기인병차재가잠、과승、강비아안문3충곤충적기인조간정현 1:1:1직향동원관계.통과비교분석발현,체절겁성결정기인재3충곤충기인조간최위보수.동과승화강비아안문상비,가잠중결정배-복부형성적기인분화최대.진일보이용심편수거대기중적12개모성기인재가잠5령제3천유충생식선중적표체정황진행분석:유2개기인(orb화nanos)재란소중특이표체;4개기인(spire、Ras85D、gd화arrest)재정소중특이표체;령외적6개기인(exuperantia、vasa、pumilio、mago nashi、nudel화dorsal)재정소화란소중균유표체,단시기중적2개기인(pumilio화nudel)재자、웅간적표체량존재현저차이.연구결과위가잠발육조공궤제적진일보연구제공료중요선색화수거신식.
Homologs for 86 developmental genes of five groups that play crucial roles in the body pattern of the Drosophila embryo were searched in the silkworm (Bombyx mori) genome by homology comparison. Results revealed that 62 (72%) orthologous genes were present in the silkworm genome and 58 (67%) of them formed orthologous groups with Anopheles gambiae genes (1:1:1 orthologous pairs). By comparison, the segment polarity group of zygotic genes was mostly conserved between the three insect genomes while the dorsal ventral group of maternal genes in B.mori seems more diversified than those found in A.gambiae. We further analyzed the expression profile of 12 silkworm maternal genes in gonads of the 3rd day fifth instar larva by using microarray data. Results showed that 2 genes (orb and nanos) and 4 genes (spire, Ras85D, gd and arrest) exclusively expressed in female and male individuals respectively. Other 6 genes (exuperantia, vasa, pumilio, mago nashi, nudel and dorsal) expressed in both female and male individuals and 2 of them (pumilio and nudel) exhibited notable differences between male and female. These results provide important information for further research in silkworm development.