CAJ | 학술논문

目的:检测贵屿电子垃圾污染区新生儿胎盘镉含量及胎盘金属硫蛋白(metallothionein, MT)表达量,评估贵屿地区新生儿镉暴露情况及对新生儿的可能影响. 方法:选取贵屿当地医院妇产科2006年7～9月出生的足月健康新生儿胎盘100例为实验组,纳入研究的产妇为贵屿镇当地居民,妊娠期间在贵屿居住.取汕头市潮南民生医院妇产科2006年5～6月出生的足月健康新生儿胎盘52例为对照组,产妇来自贵屿周边乡镇.石墨炉原子吸收法检测胎盘镉含量.链霉菌素-生物素(S-P)免疫组化技术检测胎盘组织MT的表达水平.问卷调查收集可能影响镉负荷的产妇年龄、家庭、环境、健康、饮食等因素. 结果:实验组新生儿胎盘镉水平的平均值为(0.17±0.48)μg/g,明显高于对照组(0.10±0.11)μg/g,差异具有统计学意义(P<0.01).相关分析表明产妇在贵屿居住时间、产妇妊娠期间在贵屿居住时间、产妇怀孕期间在公路附近活动时间是影响胎盘镉水平的主要因素.S-P免疫组织化学检测显示胎盘组织中蜕膜细胞、合体滋养层细胞、绒毛间质细胞均有MT的表达.实验组胎盘组织MT阳性表达率(67.00%)高于对照组(32.69%)(P<0.01).新生儿胎盘MT表达量与胎盘镉水平呈显著正相关(r=0.761,P<0.05).结论:贵屿部分新生儿处于高镉负荷状态,贵屿当地环境和从事电子垃圾作业是影响当地新生儿高镉负荷的危险因素.贵屿地区新生儿胎盘可能通过增加MT的表达拮抗镉的毒性.
목적:검측귀서전자랄급오염구신생인태반력함량급태반금속류단백(metallothionein, MT)표체량,평고귀서지구신생인력폭로정황급대신생인적가능영향. 방법:선취귀서당지의원부산과2006년7～9월출생적족월건강신생인태반100례위실험조,납입연구적산부위귀서진당지거민,임신기간재귀서거주.취산두시조남민생의원부산과2006년5～6월출생적족월건강신생인태반52례위대조조,산부래자귀서주변향진.석묵로원자흡수법검측태반력함량.련매균소-생물소(S-P)면역조화기술검측태반조직MT적표체수평.문권조사수집가능영향력부하적산부년령、가정、배경、건강、음식등인소. 결과:실험조신생인태반력수평적평균치위(0.17±0.48)μg/g,명현고우대조조(0.10±0.11)μg/g,차이구유통계학의의(P<0.01).상관분석표명산부재귀서거주시간、산부임신기간재귀서거주시간、산부부잉기간재공로부근활동시간시영향태반력수평적주요인소.S-P면역조직화학검측현시태반조직중세막세포、합체자양층세포、융모간질세포균유MT적표체.실험조태반조직MT양성표체솔(67.00%)고우대조조(32.69%)(P<0.01).신생인태반MT표체량여태반력수평정현저정상관(r=0.761,P<0.05).결론:귀서부분신생인처우고력부하상태,귀서당지배경화종사전자랄급작업시영향당지신생인고력부하적위험인소.귀서지구신생인태반가능통과증가MT적표체길항력적독성.
OBJECTIVE: This study was designed to determine the placental cadmium (Cd) levels and the expression of placental metallothionein (MT), and to investigate Cd exposure levels and its effect on neonates who lived in Guiyu, anelectronic waste (e-waste) environmental polluted site. METHODS: Among the 152 placentas included in the study, 100placentas come from pregnant women who lived in Guiyu (Exposed Group), 52 lived in chaonan, located 10 km away from Guiyu and residents had never been exposed to e-waste pollution (Control Group). Placental Cd levels were measured byatomic absorption spectrometry. Presence of placental MT was determined by immunohistochemistry. Information on maternaland neonatal characteristics and exposure conditions were obtained from hospital records and by personal interviews. RESULTS: In the Exposed Group, mean placental Cd was (0.17±0.48) μg/g, higher than Control Groups (0.10±0.11)μg/g, (P<0.01). The high levels of placental Cd were significantly associated with their occupational and environmental exposure to e-waste recycling pollutants. MT staining was positive and very dense in 67.00% (67/100) of cases in Exposed Group compared to 32.69% (17/52) in Control Group (P≤0.01). CONCLUSION: Exposure to e-waste recycling pollutants increased Cd exposure in neonates, which was accompanied by increased placental MT expression. Our finding of increased placental MT in Guiyu revealed the impact of pollution and the sensitivity of the placenta to Cd exposure inthe environment.