CAJ | 학술논문

目的对2004-2006年上海市麻疹暴发、流行的野毒株进行基因特征分析和分子流行病学研究,为提出更好的免疫策略,实现麻疹消除目标提供科学依据.方法采集的血清用ELISA法检测麻疹病毒IgM抗体;用Vero/SLAM细胞对采集的咽拭子标本进行病毒分离,分离到的麻疹病毒株用RT-PCR方法扩增其核蛋白基因C端的部分序列,并对扩增产物进行核苷酸序列测定和分析,以C端456个核苷酸为目的片段进行基因亲缘性关系分析.结果 2004-2006年上海市疾病预防控制中心对148例疑似麻疹病人的血清进行麻疹IgM抗体检测,132份血清阳性,148份咽拭子中分离到66株麻疹野病毒,阳性率分别为89.2%和44.6%.病毒分离阳性标本的对应血清麻疹IgM抗体检测均为阳性.分离到的66株麻疹野毒株均为H1基因型,除Shanghai04-5为H1b基因亚型外,其余均为H1a基因亚型.所有分离毒株之间的核苷酸同源性为95.8%～100%,氨基酸同源性为95.8%～100%.所有毒株与中国疫苗株S191的核苷酸同源性为91.2%～93.2%,氨基酸同源性为86.8%～90.1%;与疫苗株Changchun-47的核苷酸同源性为92.5%～93.4%,氨基酸同源性为88.2%～91.4%.结论引起2004-2006年上海市麻疹暴发、流行的野毒株为HI基因型,并以H1a为绝对优势亚型.上海市2005年和2006年可能都有一个主传播链的存在.
목적 대2004-2006년상해시마진폭발、류행적야독주진행기인특정분석화분자류행병학연구,위제출경호적면역책략,실현마진소제목표제공과학의거.방법 채집적혈청용ELISA법검측마진병독IgM항체;용Vero/SLAM세포대채집적인식자표본진행병독분리,분리도적마진병독주용RT-PCR방법확증기핵단백기인C단적부분서렬,병대확증산물진행핵감산서렬측정화분석,이C단456개핵감산위목적 편단진행기인친연성관계분석.결과 2004-2006년상해시질병예방공제중심대148례의사마진병인적혈청진행마진IgM항체검측,132빈혈청양성,148빈인식자중분리도66주마진야병독,양성솔분별위89.2%화44.6%.병독분리양성표본적대응혈청마진IgM항체검측균위양성.분리도적66주마진야독주균위H1기인형,제Shanghai04-5위H1b기인아형외,기여균위H1a기인아형.소유분리독주지간적핵감산동원성위95.8%～100%,안기산동원성위95.8%～100%.소유독주여중국역묘주S191적핵감산동원성위91.2%～93.2%,안기산동원성위86.8%～90.1%;여역묘주Changchun-47적핵감산동원성위92.5%～93.4%,안기산동원성위88.2%～91.4%.결론 인기2004-2006년상해시마진폭발、류행적야독주위HI기인형,병이H1a위절대우세아형.상해시2005년화2006년가능도유일개주전파련적존재.
Objective To carry out genetic characterization and molecular epidemiology study of the measles viruses(MV)isolated in shanghai from 2004 to 2006 for providing scientific basis for better immunization strategy and the goal of measles elimination.Methods Serum specimens were collected from the patients with suspected measles to detect anti-measles IgM by ELISA.Part of the nucleotide sequences of the C terminus of nucleoprotein(N)gene of these isolates were amplified by RT-PCR,and the amplicons were directly sequenced.The phylogenetic analysis was based on the nucleotide sequence about 456 bp of the C terminus of nucleoprotein(N)gene.Results Anti-measles IgM was detected in 132 of 148 serum specimens,and MV was isolated from 66 of 148 throat swabs,positive rates were 89.2% and 44.6%,respectively.The 66 strains of MV were genotype H1.All of them were subgenotype H1a except Shanghai04-5,which was subgenotype H1b.The sequence identity of 66 isolates showed that the homology of nucleotide sequences was 95.8%-100%,and that of amino acid sequences was 95.8%-100%.The homologies of nucleotide and amino acid sequences were 91.2%-93.2%and 86.8%-90.1%,respectively,when compared with that of vaccine strain S191,and those were 92.5%-93.4%and 88.2%-91.4% respectively when compared with vaccine strain changchun-47.Conclusions The wild-type MV strains circulating in shanghai were genotype H1 in 2004-2006,and the subgenotype H1a was the predominant one.There was one main transmission chain in 2005 and 2006,respectively,in shanghai.