CAJ | 학술논문

目的研究五鹤续断(WHDA)注射液对辐射损伤小鼠的保护作用.方法将160只昆明种雌性小鼠分为正常对照组、照射组和低剂量WHDA+照射组、高剂量WHDA+照射组,观察其30 d存活率;测定辐射后6 h、3 d、6 d、9 d小鼠的外周血象;测定辐射后小鼠的胸腺、脾脏和肝脏质量;测定辐射后小鼠外周血和肝脏的超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及丙二酰二醛(MDA)水平.结果 WHDA可提高辐射后小鼠的存活率,对其白细胞及血小板的数量变化有显著影响,但对其胸腺、脾脏、肝脏质量均无明显影响.WHDA还能显著提高辐射后小鼠的SOD活性,增加GSH水平,降低MDA水平.结论 WHDA对辐射损伤小鼠有明显的保护作用.
목적 연구오학속단(WHDA)주사액대복사손상소서적보호작용.방법 장160지곤명충자성소서분위정상대조조、조사조화저제량WHDA+조사조、고제량WHDA+조사조,관찰기30 d존활솔;측정복사후6 h、3 d、6 d、9 d소서적외주혈상;측정복사후소서적흉선、비장화간장질량;측정복사후소서외주혈화간장적초양화물기화매(SOD)、곡광감태(GSH)급병이선이철(MDA)수평.결과 WHDA가제고복사후소서적존활솔,대기백세포급혈소판적수량변화유현저영향,단대기흉선、비장、간장질량균무명현영향.WHDA환능현저제고복사후소서적SOD활성,증가GSH수평,강저MDA수평.결론 WHDA대복사손상소서유명현적보호작용.
Objective To study protective effect of Wu-He Dipsacus asper(WHDA) injection on the radiation damaged mice. Methods One hundred and twenty Kunming female mice were divided into normal control group, model control group, Wu-He Dipsacus asper treatment group to observe the 30-day survival rate;measured peripheral blood 6 hours, 3 days, 6 days and 9 days after irradiation, respectively; thymus, spleen and liver weight were detected; superoxide dismutase (SOD), glutathione (GSH), malonyldialdehyde (MDA) in liver and peripheral blood were also detected. Results WHDA can improve the survival rate of mice after radiation, and take a significant influence on white blood cells and platelets, but no obvious effect on the weight of thymus, spleen and liver. In addition, WHDA can improve SOD activity, increased GSH content and decrease MDA content significantly in this study. Conclusion WHDA has obvious protective effect in mice with radiation injury.