中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
6期
725-728
,共4页
陆平%李云鹏%张万峰%李达敏
陸平%李雲鵬%張萬峰%李達敏
륙평%리운붕%장만봉%리체민
原癌基因蛋白质c-pim-1/代谢%乳腺肿瘤/代谢/病理学
原癌基因蛋白質c-pim-1/代謝%乳腺腫瘤/代謝/病理學
원암기인단백질c-pim-1/대사%유선종류/대사/병이학
Proto-oncogene proteins c-pim-1/metabolism%Breast neoplasms/metabolism/pathology
目的 探讨PIM-1蛋白激酶在乳腺癌组织、乳腺非典型增生组织及正常乳腺组织中的表达及生物学意义.方法 利用免疫组织化学、Western blot方法检测乳腺癌组织、乳腺非典型增生组织和正常乳腺组织中PIM-1蛋白激酶的表达水平.结果 乳腺癌组织、乳腺非典型增生组织和正常乳腺组织中PIM-1蛋白激酶的阳性表达率分别为78.75％ (63/80)、42.00％ (21/50)、23.33％ (7/30);乳腺癌组织中PIM-1的蛋白水平明显高于癌旁正常乳腺组织(灰度值:118.807±19.99 vs 103.062±13.51,t=8.578,P＜0.05);PIM-1蛋白激酶的表达与乳腺癌的P-TNM分期和淋巴结转移密切相关(P=0.004,P=0.003),与年龄和肿块大小无关(P=0.654,P=0.084).结论 PIM-1在乳腺癌中表达阳性率明显高于非典型增生的乳腺组织和正常乳腺组织,PIM-1可能是乳腺癌形成过程中的早期分子事件,其表达增强与乳腺癌的浸润性发展密切相关,有可能成为新的早期筛查和判断乳腺癌生物学行为的诊断指标.利用石蜡标本检测指标是可行的,具有方便,易得,经济的特点.
目的 探討PIM-1蛋白激酶在乳腺癌組織、乳腺非典型增生組織及正常乳腺組織中的錶達及生物學意義.方法 利用免疫組織化學、Western blot方法檢測乳腺癌組織、乳腺非典型增生組織和正常乳腺組織中PIM-1蛋白激酶的錶達水平.結果 乳腺癌組織、乳腺非典型增生組織和正常乳腺組織中PIM-1蛋白激酶的暘性錶達率分彆為78.75％ (63/80)、42.00％ (21/50)、23.33％ (7/30);乳腺癌組織中PIM-1的蛋白水平明顯高于癌徬正常乳腺組織(灰度值:118.807±19.99 vs 103.062±13.51,t=8.578,P＜0.05);PIM-1蛋白激酶的錶達與乳腺癌的P-TNM分期和淋巴結轉移密切相關(P=0.004,P=0.003),與年齡和腫塊大小無關(P=0.654,P=0.084).結論 PIM-1在乳腺癌中錶達暘性率明顯高于非典型增生的乳腺組織和正常乳腺組織,PIM-1可能是乳腺癌形成過程中的早期分子事件,其錶達增彊與乳腺癌的浸潤性髮展密切相關,有可能成為新的早期篩查和判斷乳腺癌生物學行為的診斷指標.利用石蠟標本檢測指標是可行的,具有方便,易得,經濟的特點.
목적 탐토PIM-1단백격매재유선암조직、유선비전형증생조직급정상유선조직중적표체급생물학의의.방법 이용면역조직화학、Western blot방법검측유선암조직、유선비전형증생조직화정상유선조직중PIM-1단백격매적표체수평.결과 유선암조직、유선비전형증생조직화정상유선조직중PIM-1단백격매적양성표체솔분별위78.75％ (63/80)、42.00％ (21/50)、23.33％ (7/30);유선암조직중PIM-1적단백수평명현고우암방정상유선조직(회도치:118.807±19.99 vs 103.062±13.51,t=8.578,P＜0.05);PIM-1단백격매적표체여유선암적P-TNM분기화림파결전이밀절상관(P=0.004,P=0.003),여년령화종괴대소무관(P=0.654,P=0.084).결론 PIM-1재유선암중표체양성솔명현고우비전형증생적유선조직화정상유선조직,PIM-1가능시유선암형성과정중적조기분자사건,기표체증강여유선암적침윤성발전밀절상관,유가능성위신적조기사사화판단유선암생물학행위적진단지표.이용석사표본검측지표시가행적,구유방편,역득,경제적특점.
Objective To investigate the expression and biological significance of PIM1 in the breast cancer,atypical hyperplasia breast,and normal breast tissues.Methods The protein expression levels of PIM1 in the breast cancer,atypical hyperplasia breast,and normal breast tissues were assayed by immunohistochemistry and Western blot.Results The expression rates of PIM1 protein were 78.75％ (63/80)in breast cancers,42.00％ (21/50) in atypical hyperplasia tissues,and 23.33 ％ (7/30) in normal breast tissues,respectively.A significant correlation was found between PIM1 expression and the clinical stage and lymphonodus metastasis.However,no significant correlation was found between PIM1 expression and patients'age and tumor sizes.The level of PIM1 protein in breast cancers was obviously higher than that in non - cancerous tissues.Conclusions The positive rate of PIM1 in breast cancers is significantly higher than that in atypical hyperplasia breast tissues and in normal breast tissues.PIM1 may be an early molecular event in mammary tumorigenesis,and its overexpression may significantly relate to the malignant progression.It would be a new parameter for the early diagnosis and a biomarker for breast cancers.It is feasible to utilize paraffin specimens for index test with advantages of convenience,easy availability,and low expense.
