中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
5期
276-279
,共4页
刘景%钟良军%史芳川%张蕾%张琼%孙大磊
劉景%鐘良軍%史芳川%張蕾%張瓊%孫大磊
류경%종량군%사방천%장뢰%장경%손대뢰
牙周炎%早产%模型,动物%肿瘤坏死因子类
牙週炎%早產%模型,動物%腫瘤壞死因子類
아주염%조산%모형,동물%종류배사인자류
Periodontitis%Premature birth%Models,animal%Tumor necrosis factors
目的 构建孕鼠慢性牙周炎(chronic periodontitis,CP)模型,探讨肿瘤坏死因子(TNF)在CP孕鼠早产胎盘组织中的表达.方法 选取60 d龄Wistar雌鼠20只按随机数字表分为两组:正常对照组8只、CP组12只,CP组建立牙周炎模型,4周后两组雌鼠均与雄鼠合笼,观察两组孕鼠的分娩时间及新生鼠出生体质量,至孕19 d(大鼠足孕期为21～23 d,早于孕19 d即为早产)处死全部孕鼠取胎盘组织.观察孕鼠牙周组织的病理改变;实时荧光定量聚合酶链反应(PCR)法分析胎盘组织中TNF的表达.结果 实验牙的牙周检查及组织病理学观察结果 显示,已成功建立了孕鼠CP动物模型.CP组共有7只孕鼠提前分娩,活产仔鼠30只;正常对照组至孕19 d处死时,活产仔鼠56只.CP组早产孕鼠每胎平均活产仔鼠数[(4.1±2.2)只]显著低于正常对照组[(9.2±2.2)只],CP组仔鼠出生体质量[(5.01±0.43)g]也显著低于正常对照组[(5.79±0.53)g],P<0.05.TNF基因在CP组和正常对照组的相对定量表达值分别为(1.807±0.265)、(1.003±0.021),差异有统计学意义(P=0.001).结论 TNF在慢性牙周炎孕鼠胎盘组织中呈过表达,它与孕鼠的早产及仔鼠出生体质量低有一定关系.
目的 構建孕鼠慢性牙週炎(chronic periodontitis,CP)模型,探討腫瘤壞死因子(TNF)在CP孕鼠早產胎盤組織中的錶達.方法 選取60 d齡Wistar雌鼠20隻按隨機數字錶分為兩組:正常對照組8隻、CP組12隻,CP組建立牙週炎模型,4週後兩組雌鼠均與雄鼠閤籠,觀察兩組孕鼠的分娩時間及新生鼠齣生體質量,至孕19 d(大鼠足孕期為21～23 d,早于孕19 d即為早產)處死全部孕鼠取胎盤組織.觀察孕鼠牙週組織的病理改變;實時熒光定量聚閤酶鏈反應(PCR)法分析胎盤組織中TNF的錶達.結果 實驗牙的牙週檢查及組織病理學觀察結果 顯示,已成功建立瞭孕鼠CP動物模型.CP組共有7隻孕鼠提前分娩,活產仔鼠30隻;正常對照組至孕19 d處死時,活產仔鼠56隻.CP組早產孕鼠每胎平均活產仔鼠數[(4.1±2.2)隻]顯著低于正常對照組[(9.2±2.2)隻],CP組仔鼠齣生體質量[(5.01±0.43)g]也顯著低于正常對照組[(5.79±0.53)g],P<0.05.TNF基因在CP組和正常對照組的相對定量錶達值分彆為(1.807±0.265)、(1.003±0.021),差異有統計學意義(P=0.001).結論 TNF在慢性牙週炎孕鼠胎盤組織中呈過錶達,它與孕鼠的早產及仔鼠齣生體質量低有一定關繫.
목적 구건잉서만성아주염(chronic periodontitis,CP)모형,탐토종류배사인자(TNF)재CP잉서조산태반조직중적표체.방법 선취60 d령Wistar자서20지안수궤수자표분위량조:정상대조조8지、CP조12지,CP조건립아주염모형,4주후량조자서균여웅서합롱,관찰량조잉서적분면시간급신생서출생체질량,지잉19 d(대서족잉기위21～23 d,조우잉19 d즉위조산)처사전부잉서취태반조직.관찰잉서아주조직적병리개변;실시형광정량취합매련반응(PCR)법분석태반조직중TNF적표체.결과 실험아적아주검사급조직병이학관찰결과 현시,이성공건립료잉서CP동물모형.CP조공유7지잉서제전분면,활산자서30지;정상대조조지잉19 d처사시,활산자서56지.CP조조산잉서매태평균활산자서수[(4.1±2.2)지]현저저우정상대조조[(9.2±2.2)지],CP조자서출생체질량[(5.01±0.43)g]야현저저우정상대조조[(5.79±0.53)g],P<0.05.TNF기인재CP조화정상대조조적상대정량표체치분별위(1.807±0.265)、(1.003±0.021),차이유통계학의의(P=0.001).결론 TNF재만성아주염잉서태반조직중정과표체,타여잉서적조산급자서출생체질량저유일정관계.
Objective To examine the expression of tumor necrosis factor in placenta of pregnant rats with chronic periodontitis.Methods Twenty Wistar female rats were randomly divided into two groups,control(n=8)and experimentalgroup(n=12).The periodontitis model was established in the experimental group.The females and males in the two groups got together four weeks later.Nineteen days after pregnancy all rats were executed and placenta collected.The delivery time and neonatal birth weight were recorded and the pathological changes of periodontal tissue observed.Tumor necrosis factor(TNF)expression was examined in placenta by real-time quantitative polymerase chain reaction analysis.Results The animal model of chronic periodontitis was successfully established.Experimental group delivered 30 offspring and the control group 56 offspring.The average number of pups born alive per liner in experimental group(4.1±2.2)Was significantly lower than that in control group(9.2±2.2),P<0.05.The birth weight of pups in experimental group[(5.01±0.43)g]wag significantly lower than that in the control group[(5.79±0.53)g],P<0.05.The relative quantitative expression of TNF was(1.807±0.265)in experimental group the and (1.003±0.021)in the control group(P=0.001).Conclusions Chronic periodonfitis may be related to preterm low birth weight.
