中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
37期
2625-2628
,共4页
巨噬细胞移动抑制因子%腺癌%胃%免疫组织化学
巨噬細胞移動抑製因子%腺癌%胃%免疫組織化學
거서세포이동억제인자%선암%위%면역조직화학
Macrophage migration inhibitory factor%Adenocarcinoma%Stomach%Immunohistochemistry
目的 检测巨噬细胞移动抑制因子(MIF)在不同分化程度胃腺癌组织、癌旁组织及正常胃黏膜组织中的表达,分析MIF的表达与胃腺癌分化程度、淋巴结转移情况及其他临床病理特征相关性.方法 选择胃腺癌组织120例及癌旁组织40例为实验组,其中高、中、低分化腺癌各40例,选择正常胃黏膜组织40例为对照组.应用免疫组化法检测不同分化程度胃腺癌组织、癌旁组织、正常胃黏膜组织中MIF表达情况.结果 胃腺癌MIF表达阳性率为80.8%(97/120),低分化、中分化、高分化胃腺癌MIF表达阳性率分别为97.5%(39/40),82.5%(33/40),62.5%(25/40).癌旁组织MIF表达阳性率为40%(16/40),正常胃黏膜MIF表达阳性率为7.5%(3/40).低、中、高分化胃腺癌MIF表达阳性率Spearman等级相关分析系数rs为0.458(x2=27.046,P＜0.001).MIF的表达与临床病理因素关系相关性为淋巴结转移x2=9.231(P=0.002)、性别x2=0.045(P=0.832)、年龄为x2=0.241(P=0.623)、肿瘤大小x2=0.003(P=0.954)、肿瘤部位x2=0.051(P=0.821).结论 胃腺癌组织MIF表达明显高于癌旁组织及正常胃黏膜组织MIF表达,低分化腺癌MIF表达明显高于高/中分化腺癌MIF表达.MIF表达与胃腺癌组织分化程度及淋巴是否转移密切相关,与年龄、性别、肿瘤大小、肿瘤部位无相关性.
目的 檢測巨噬細胞移動抑製因子(MIF)在不同分化程度胃腺癌組織、癌徬組織及正常胃黏膜組織中的錶達,分析MIF的錶達與胃腺癌分化程度、淋巴結轉移情況及其他臨床病理特徵相關性.方法 選擇胃腺癌組織120例及癌徬組織40例為實驗組,其中高、中、低分化腺癌各40例,選擇正常胃黏膜組織40例為對照組.應用免疫組化法檢測不同分化程度胃腺癌組織、癌徬組織、正常胃黏膜組織中MIF錶達情況.結果 胃腺癌MIF錶達暘性率為80.8%(97/120),低分化、中分化、高分化胃腺癌MIF錶達暘性率分彆為97.5%(39/40),82.5%(33/40),62.5%(25/40).癌徬組織MIF錶達暘性率為40%(16/40),正常胃黏膜MIF錶達暘性率為7.5%(3/40).低、中、高分化胃腺癌MIF錶達暘性率Spearman等級相關分析繫數rs為0.458(x2=27.046,P＜0.001).MIF的錶達與臨床病理因素關繫相關性為淋巴結轉移x2=9.231(P=0.002)、性彆x2=0.045(P=0.832)、年齡為x2=0.241(P=0.623)、腫瘤大小x2=0.003(P=0.954)、腫瘤部位x2=0.051(P=0.821).結論 胃腺癌組織MIF錶達明顯高于癌徬組織及正常胃黏膜組織MIF錶達,低分化腺癌MIF錶達明顯高于高/中分化腺癌MIF錶達.MIF錶達與胃腺癌組織分化程度及淋巴是否轉移密切相關,與年齡、性彆、腫瘤大小、腫瘤部位無相關性.
목적 검측거서세포이동억제인자(MIF)재불동분화정도위선암조직、암방조직급정상위점막조직중적표체,분석MIF적표체여위선암분화정도、림파결전이정황급기타림상병리특정상관성.방법 선택위선암조직120례급암방조직40례위실험조,기중고、중、저분화선암각40례,선택정상위점막조직40례위대조조.응용면역조화법검측불동분화정도위선암조직、암방조직、정상위점막조직중MIF표체정황.결과 위선암MIF표체양성솔위80.8%(97/120),저분화、중분화、고분화위선암MIF표체양성솔분별위97.5%(39/40),82.5%(33/40),62.5%(25/40).암방조직MIF표체양성솔위40%(16/40),정상위점막MIF표체양성솔위7.5%(3/40).저、중、고분화위선암MIF표체양성솔Spearman등급상관분석계수rs위0.458(x2=27.046,P＜0.001).MIF적표체여림상병리인소관계상관성위림파결전이x2=9.231(P=0.002)、성별x2=0.045(P=0.832)、년령위x2=0.241(P=0.623)、종류대소x2=0.003(P=0.954)、종류부위x2=0.051(P=0.821).결론 위선암조직MIF표체명현고우암방조직급정상위점막조직MIF표체,저분화선암MIF표체명현고우고/중분화선암MIF표체.MIF표체여위선암조직분화정도급림파시부전이밀절상관,여년령、성별、종류대소、종류부위무상관성.
Objective To investigate the expression of MIF on the gastric adenocarcinoma, evaluate the relation between the MIF expression and tumor differentiation, lymph node metastasis and other clinical patho-features. Methods 120 gastric adenocarcinoma cancer tissues, 40 tissues besides cancer and 40common mucosa tissues specimens are collected. Detect the expression of MIF with immunohistochemistry method (Streptavidin -Peroxidase). Results Positive rate of MIF expression in gastric cancer is 80. 8%(97/120). Those in poorly differentiated, moderately differentiated and well-differentiated gastric adenocarcinoma are 97. 5% (39/40), 82. 5% (33/40) ,62.5% (25/40), analytical factor of Spearman rank correlation rs is 0. 458( x2 =27. 046, P ＜0.001 ). Positive rate of MIF expression in tissues besides cancer is 40% (16/40)and that in normal gastric mucosa is 7.5% (3/40). Positive rate of MIF expression in gastric adenocarcinoma without lymph node metastasis is 40% (22/55) and the rate with lymph node metastasis is 67% (44/65). Conclusions There is overexpression of MIF in the gastric adenocarcinoma.Expression of MIF in different differentiated cancer is different. Expression in poorly differentiated is higher than that in moderately differentiated and well-differentiated caner. The level of expression of MIF malignancy has positive correlation with malignancy degree of gastric adenocarcinoma. MIF expression has nothing to do with age, gender, tumor sizes or location.
