CAJ | 학술논문

目的建立血小板谱抗原,鉴定引起血小板输注无效和新生儿血小板减少性紫癜的血小板特异性抗体,为血小板血型研究和临床治疗提供依据。方法根据中国人群人类血小板同种抗原(HPA)-1-HPA-16等位基因频率分布资料,利用聚合酶链反应-序列特异引物(PCR-SSP)技术对O型血小板供者进行HPA-1-HPA-6、HPA-15分型,筛选合适的供者,组成血小板谱抗原。通过建立的血小板谱抗原,利用简易致敏红细胞血小板血清学技术(SEPSA)鉴定同种免疫反应产生的血小板抗体的特异性。结果从O型血小板供者中筛选出11名供者,建立了血小板特异性抗体鉴定谱抗原。其可鉴定HPA-1-HPA-6,HPA-15抗体的特异性。在所筛检1 120份样本中,有3例患者检出HPA抗体,其中HPA-4b(Penb)抗体1例,HPA-15a(Govb)抗体2例。结论通过血小板谱抗原鉴定血小板抗体的特异性,对提高临床输注血小板的安全性和有效性,以及预防新生儿血小板减少性紫癜有积极的意义.
목적 건립혈소판보항원,감정인기혈소판수주무효화신생인혈소판감소성자전적혈소판특이성항체,위혈소판혈형연구화림상치료제공의거。방법근거중국인군인류혈소판동충항원(HPA)-1-HPA-16등위기인빈솔분포자료,이용취합매련반응-서렬특이인물(PCR-SSP)기술대O형혈소판공자진행HPA-1-HPA-6、HPA-15분형,사선합괄적공자,조성혈소판보항원。통과건립적혈소판보항원,이용간역치민홍세포혈소판혈청학기술(SEPSA)감정동충면역반응산생적혈소판항체적특이성。결과종O형혈소판공자중사선출11명공자,건립료혈소판특이성항체감정보항원。기가감정HPA-1-HPA-6,HPA-15항체적특이성。재소사검1 120빈양본중,유3례환자검출HPA항체,기중HPA-4b(Penb)항체1례,HPA-15a(Govb)항체2례。결론통과혈소판보항원감정혈소판항체적특이성,대제고림상수주혈소판적안전성화유효성,이급예방신생인혈소판감소성자전유적겁적의의.
Objective To establish the platelet antigen panel for identifying the specificity of platelet antibodies which cause platelet transfusion refractoriness and neonatal alloimmune thrombocytopenia and provide evidence for clinical therapy and platelet genotyping research.Methods Based on the frequency distribution of human platelet alloantigen (HPA)-1 to HPA-16 gene in China, the frequencies of HPA-1 to HPA-6,HPA-15 alleles in blood group O donors were genotyped by the polymerase chain reaction with sequence-specific primers (PCR-SSP) method, and suitable donors were chosen to establish platelet-specific antigen panel.Using the established platelet-specific antigen panel, the specificity of platelet antibodies caused by alloimmune reaction was identified by using simplified sensitized erythrocyte platelet serology assay (SEPSA).Results Eleven ptatelet donors with blood group O were chosen to establish platelet-specific antigen panel which can identify specificity of HPA-1 to HPA-6, HPA-15 antibodies.One case of HPA-4b (Penb) and two cases of HPA-15a (Govb) platelet specific antibodies were detected in 1 120 samples.Conclusion Identifying the specific platelet antibodies using platelet specific antigen panel has profound significance on increasing the safety and effectiveness of clinical platelet transfusion and prevention of neonatal alloimmune thrombocytopenia.