肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
4期
227-230
,共4页
兰小琴%王莹%李美宁%张悦红%梁小波%程牛亮
蘭小琴%王瑩%李美寧%張悅紅%樑小波%程牛亮
란소금%왕형%리미저%장열홍%량소파%정우량
地诺前列酮%结肠肿瘤%SW480细胞
地諾前列酮%結腸腫瘤%SW480細胞
지낙전렬동%결장종류%SW480세포
Dinoprastone%Colonic neoplasms%SW480 cells
目的 研究前列腺素E2(PGE2)对结肠癌SW480细胞黏附、迁移、侵袭能力的影响及其可能的分子机制.方法 分别使用外源性PGE2及其受体EP1的拮抗剂SC19220作用于SW480细胞后,MTr法测定肿瘤细胞黏附能力,transwell小室检测细胞迁移及侵袭能力,RT-PCR及Western blotting检测血管内皮生长因子(VEGF)mRNA和蛋白水平的改变.结果 外源性PGE2可以显著增加SW480细胞黏附、迁移及侵袭能力,PGE2作用于细胞后,黏附细胞A值由0.207±0.009增加至0.417±0.088,迁移细胞由6.33±0.33增加至43.33±0.88,侵袭细胞由3.67±0.34增加至26.33±0.89,差异均有统计学意义(P＜0.05).SC19220可抑制PGE2所诱导的SW480细胞黏附、迁移及侵袭,PGE2+SC19220组与PGE2组相比,黏附细胞A值由0.417±0.088下降至0.140±0.006,迁移细胞由43.33±0.88下降至28.00±0.58,侵袭细胞由26.33±0.89下降至5.67±0.33,差异均有统计学差异(P＜0.05).RT-PCR及Western blotting检测结果显示,PGE2作用后SW480细胞VEGFmRNA及蛋白水平表达增强,且表达量与PGE2浓度呈正相关.结论 PGE2可显著增加SW480细胞黏附、迁移及侵袭能力,其作用过程可能与VEGF表达上调相关.
目的 研究前列腺素E2(PGE2)對結腸癌SW480細胞黏附、遷移、侵襲能力的影響及其可能的分子機製.方法 分彆使用外源性PGE2及其受體EP1的拮抗劑SC19220作用于SW480細胞後,MTr法測定腫瘤細胞黏附能力,transwell小室檢測細胞遷移及侵襲能力,RT-PCR及Western blotting檢測血管內皮生長因子(VEGF)mRNA和蛋白水平的改變.結果 外源性PGE2可以顯著增加SW480細胞黏附、遷移及侵襲能力,PGE2作用于細胞後,黏附細胞A值由0.207±0.009增加至0.417±0.088,遷移細胞由6.33±0.33增加至43.33±0.88,侵襲細胞由3.67±0.34增加至26.33±0.89,差異均有統計學意義(P＜0.05).SC19220可抑製PGE2所誘導的SW480細胞黏附、遷移及侵襲,PGE2+SC19220組與PGE2組相比,黏附細胞A值由0.417±0.088下降至0.140±0.006,遷移細胞由43.33±0.88下降至28.00±0.58,侵襲細胞由26.33±0.89下降至5.67±0.33,差異均有統計學差異(P＜0.05).RT-PCR及Western blotting檢測結果顯示,PGE2作用後SW480細胞VEGFmRNA及蛋白水平錶達增彊,且錶達量與PGE2濃度呈正相關.結論 PGE2可顯著增加SW480細胞黏附、遷移及侵襲能力,其作用過程可能與VEGF錶達上調相關.
목적 연구전렬선소E2(PGE2)대결장암SW480세포점부、천이、침습능력적영향급기가능적분자궤제.방법 분별사용외원성PGE2급기수체EP1적길항제SC19220작용우SW480세포후,MTr법측정종류세포점부능력,transwell소실검측세포천이급침습능력,RT-PCR급Western blotting검측혈관내피생장인자(VEGF)mRNA화단백수평적개변.결과 외원성PGE2가이현저증가SW480세포점부、천이급침습능력,PGE2작용우세포후,점부세포A치유0.207±0.009증가지0.417±0.088,천이세포유6.33±0.33증가지43.33±0.88,침습세포유3.67±0.34증가지26.33±0.89,차이균유통계학의의(P＜0.05).SC19220가억제PGE2소유도적SW480세포점부、천이급침습,PGE2+SC19220조여PGE2조상비,점부세포A치유0.417±0.088하강지0.140±0.006,천이세포유43.33±0.88하강지28.00±0.58,침습세포유26.33±0.89하강지5.67±0.33,차이균유통계학차이(P＜0.05).RT-PCR급Western blotting검측결과현시,PGE2작용후SW480세포VEGFmRNA급단백수평표체증강,차표체량여PGE2농도정정상관.결론 PGE2가현저증가SW480세포점부、천이급침습능력,기작용과정가능여VEGF표체상조상관.
Objective To investigate whether prostaglandin E2(PGE2) can promote the ability of adhesion.migration and invasion of colorectal cancer cells SW480 and its mechanism.Methods Extrinsic source PGE2 and the antagonist of EP1 SC19220 were added to the culture media. MTr assay was used to identify the adhesion ability of SW480 cells. Migration ability was tested by transwell plate.The invasion ability was tested bv ECM gel coated transweU plate. RT-PCR and western blotting were used to detect the mRNA and protein level of vascular endothelial growth factor (VEGF). Results The adhesion.invasion and migration ratio of SW480 ceils were all increased significantly after treated with PGEh the A values of adhesion ceils increased from 0.207±0.009 to 0.417±0.088. Migration cells increased from 6.33±0.33 to 43.33±0.88.invasion cells increased from 3.67±0.34 to 26.33±0.89(P＜0.05).The adhesion.migration and invasion cells of PGE2+SC 19220 group decreased significantly compared to PGE2 group.The A values of adhesion cells decreased from 0.417±0.088 to 0.140±0.006. Migration cells decreased from 43.33±0.88 to 28.00±0.58.invasion cells decreased from 26.33±0.89 to 5.67±0.33 (P＜0.05).The results of RT-PCR and western blotting showed that the expression of VEGF mRNA and protein increased in a dose dependent manner after PGE2 treatment. Conclusion The ability of adhesion. Migration and invasion of SW480increased after PGE2 was added to the culture media.It may be related to the upregulation of VEGF.
