CAJ | 학술논문

本研究的目的是证实抗TGF-β抗体在人脐血CD34+细胞体外扩增过程中可以增加CD34+细胞的扩增效率,并观察其对脐血CD34+细胞粘附分子表达的影响.从6份新鲜脐血标本中富集CD34+细胞,将其接种于含抗TGF-β抗体+SCF+Flt-3L+TPO+IL-3因子组合的SFEM无血清培养体系中,培养6天后,细胞计数,并用FACS检测CD34、c-kit(CD117)、CD11a、CD49d、CD33表达情况,同时进行集落分析.结果表明:①实验组有核细胞数、CD34+细胞数、CD34+c-kit+细胞数及CFU-GEMM分别扩增了41.82±13.49,15.62±6.95,13.36±6.12,11.07±4.05倍,明显高于对照组(P分别=0.001,0.002,0.003,0.002),其中实验组中更为早期的CD34+c-kitˉ亚群的扩增倍数更多(69.10±41.06),多于有核细胞、CD34+细胞、CD34+c-kit+细胞的扩增倍数(P=0.024).②TGF-β抗体对CD11a和CD49d的表达无明显影响(P值均大于0.05).结论:抗TGF-β抗体可协同早期干细胞生长因子有效扩增脐血CD34+细胞,同时不降低CD34+细胞黏附分子的表达.
본연구적목적시증실항TGF-β항체재인제혈CD34+세포체외확증과정중가이증가CD34+세포적확증효솔,병관찰기대제혈CD34+세포점부분자표체적영향.종6빈신선제혈표본중부집CD34+세포,장기접충우함항TGF-β항체+SCF+Flt-3L+TPO+IL-3인자조합적SFEM무혈청배양체계중,배양6천후,세포계수,병용FACS검측CD34、c-kit(CD117)、CD11a、CD49d、CD33표체정황,동시진행집락분석.결과표명:①실험조유핵세포수、CD34+세포수、CD34+c-kit+세포수급CFU-GEMM분별확증료41.82±13.49,15.62±6.95,13.36±6.12,11.07±4.05배,명현고우대조조(P분별=0.001,0.002,0.003,0.002),기중실험조중경위조기적CD34+c-kitˉ아군적확증배수경다(69.10±41.06),다우유핵세포、CD34+세포、CD34+c-kit+세포적확증배수(P=0.024).②TGF-β항체대CD11a화CD49d적표체무명현영향(P치균대우0.05).결론:항TGF-β항체가협동조기간세포생장인자유효확증제혈CD34+세포,동시불강저CD34+세포점부분자적표체.