CAJ | 학술논문

采用室内发芽试验方法,研究了不同浓度微囊藻毒素MC-LR对黑麦草种子发芽率、幼苗生长及幼苗体内抗氧化酶SOD、POD和CAT活性的影响,同时用液相色谱/质谱法(L/MS法)检测MC-LR在黑麦草幼苗体内的积累.结果显示,随着MC-LR处理浓度升高,黑麦草种子发芽率逐渐降低;MC-LR对黑麦草幼苗株高和干重无显著影响;但4mg·L~(-1)MC-LR处理对幼苗根长和鲜重具有显著抑制作用.MC-LR处理提高了黑麦草幼苗体内SOD和POD活性,但高浓度MC-LR对两种酶活性又具有一定抑制作用.随着MC-LR处理浓度升高,MC-LR在幼苗体内积累含量和生物富集系数逐渐增大.黑麦草幼苗体内可积累MC-LR,这有可能通过食物链途径对食品安全造成一定潜在风险.
채용실내발아시험방법,연구료불동농도미낭조독소MC-LR대흑맥초충자발아솔、유묘생장급유묘체내항양화매SOD、POD화CAT활성적영향,동시용액상색보/질보법(L/MS법)검측MC-LR재흑맥초유묘체내적적루.결과현시,수착MC-LR처리농도승고,흑맥초충자발아솔축점강저;MC-LR대흑맥초유묘주고화간중무현저영향;단4mg·L~(-1)MC-LR처리대유묘근장화선중구유현저억제작용.MC-LR처리제고료흑맥초유묘체내SOD화POD활성,단고농도MC-LR대량충매활성우구유일정억제작용.수착MC-LR처리농도승고,MC-LR재유묘체내적루함량화생물부집계수축점증대.흑맥초유묘체내가적루MC-LR,저유가능통과식물련도경대식품안전조성일정잠재풍험.
Effects of different concentrations of MC-LR on germination rate of ryegrass seeds, the growth and activity of antioxidant enzymes like SOD, POD and CAT of ryegrass seedlings were studied, and the accumulation of MC-LR in seedlings was also detected by Liquid chromatography/mass spectrometry(L/MS)method. The results showed that with the concentration of MC-LR increased, germination rate of ryegrass seeds reduced. There were no significant effects on seedlings height and dry weight of ryegrass seedlings after treated by MC-LR for seven days, hut significant effected on root length and fresh weight of ryegrass seedlings when the concentration of MC-LR was the highest of 4 mg·L~(-1). The activity of superoxide dismutase(SOD)and peroxidase (POD)increased with the MC-LR treatment when the concentration of MC-LR was lower but were inhibited when the concentration of MC-LR was higher. However, there was not a similar trend of the activity of catalase(CAT) of ryegrass seedlings observed after the MC-LR treatment. With the concentration of MC-LR increased, the contents of MC-LR accumulated in ryegrass seedlings and bioconcentration factor (BCF) were increased, respectively. The result that MC-LR could be accumulated in ryegrass seedlings suggests MC-LR may have potential food safety risk through the way of food chain as the livestock such as cattle and sheep fed on ryegrass.