CAJ | 학술논문

目的:探讨凋亡基因Bcl-2启动子区C(-938)A单核苷酸多态性(snigle nucleotide polymorphism,SNP)与河北省乳腺癌患者临床生物学指标的关系.方法:采用聚合酶链反应-限制性酶切片段长度多态性分析(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测113例乳腺癌患者Bcl-2基因 C(-938)A SNP的3个基因型(即AA、AC和CC),并与临床生物学指标相关联,比较各组间基因型频率的分布情况.结果: 按腋淋巴结转移个数进行分层分析,AA型和AC+CC型在无转移、1～3个转移、≥4个转移组中分别占26.8%、47.8%、52.6%和73.2%、52.2%、47.4%,两组比较差异有统计学意义(χ~2=6.337,P=0.042);与AC+CC型相比,AA型在≥4个转移组OR值为3.041(95%CI:1.072～8.626).在组织学分级中,AA型和AC+CC型在Ⅰ～Ⅱ级、Ⅲ级中分别占30.9%、57.9%和69.1%、42.1%,两组基因型分布差异有统计学意义(χ~2=5.055,P=0.025);与AC+CC型相比,AA型在肿瘤分化Ⅲ级组中OR值为3.082(95%CI:1.122～8.465).按雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)和C-erbB2蛋白表达水平进行分层分析,发现AA型和AC+CC型的上述指标分布差异无统计学意义(χ~2=3.005, χ~2=1.504, χ~2=1.163;P >0.05).结论:乳腺癌患者Bcl-2基因C(-938)A SNP的AA基因型与其腋淋巴结转移率较高和肿瘤组织分化较差相关.
목적:탐토조망기인Bcl-2계동자구C(-938)A단핵감산다태성(snigle nucleotide polymorphism,SNP)여하북성유선암환자림상생물학지표적관계.방법:채용취합매련반응-한제성매절편단장도다태성분석(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)방법검측113례유선암환자Bcl-2기인 C(-938)A SNP적3개기인형(즉AA、AC화CC),병여림상생물학지표상관련,비교각조간기인형빈솔적분포정황.결과: 안액림파결전이개수진행분층분석,AA형화AC+CC형재무전이、1～3개전이、≥4개전이조중분별점26.8%、47.8%、52.6%화73.2%、52.2%、47.4%,량조비교차이유통계학의의(χ~2=6.337,P=0.042);여AC+CC형상비,AA형재≥4개전이조OR치위3.041(95%CI:1.072～8.626).재조직학분급중,AA형화AC+CC형재Ⅰ～Ⅱ급、Ⅲ급중분별점30.9%、57.9%화69.1%、42.1%,량조기인형분포차이유통계학의의(χ~2=5.055,P=0.025);여AC+CC형상비,AA형재종류분화Ⅲ급조중OR치위3.082(95%CI:1.122～8.465).안자격소수체(estrogen receptor,ER)、잉격소수체(progesterone receptor,PR)화C-erbB2단백표체수평진행분층분석,발현AA형화AC+CC형적상술지표분포차이무통계학의의(χ~2=3.005, χ~2=1.504, χ~2=1.163;P >0.05).결론:유선암환자Bcl-2기인C(-938)A SNP적AA기인형여기액림파결전이솔교고화종류조직분화교차상관.
Objective:To study the correlation of C(-938)A single nucleotide polymorphism (SNP) in the promoter of anti-apoptosis gene Bcl-2 with the clinical biological parameters of breast cancer patients in Hebei Province. Methods:Three genotypes(AA, AC, CC) of Bcl-2 C(-938)A from 113 samples of breast cancer patients were analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the results were associated with clinical biological parameters. The distribution of genotype frequency was compared between different groups. Results:When stratified for axillary lymph node metastases, the frequency of AA genotype were 26.8%, 47.8% and 52.6% and the distribution of AC+CC genotypes were 73.2%, 52.2% and 47.4% in negative group, 1-3 metastasis group, and ≥4 metastasis group. The difference between the two groups was significant (χ~2=6.337, P=0.042). Compared with the AC+CC genotypes, the OR value of AA genotype in ≥4 metastasis group was 3.041 (95%CI=1.072-8.626). The frequency of AA genotype were 30.9% and 69.1% in gradeⅠ-Ⅱ group and grade Ⅲ group, and the frequency of AC+CC genotypes were 57.9% and 42.1%. The difference between the two groups was significant (χ~2=5.055; P=0.025). Compared with the AC+CC genotypes, the OR value of AA genotype in differentiated tumors(grade Ⅲ)was 3.082 (95%CI=1.122-8.465). Stratified for estrogen receptor (ER), progesterone receptor (PR) and C-erbB2, there was no difference between the distribution of AA genotype and AC+CC genotypes (χ~2=3.005, χ~2=1.504, χ~2=1.163, P>0.05). Conclusion:The AA genotype of Bcl-2 gene C(-938)A maybe correlated with high lymph node metastasis rate and poor differentiation.