CAJ | 학술논문

生物质谱是蛋白质组研究中的核心技术之一,可以实现大规模、高通量的蛋白质定性和定量分析.由于样品和实验过程自身的复杂性,质谱实验的重复性还存在一些问题,肽段鉴定和定量结果有很大的随机性,肽段的质谱检测概率问题在蛋白质组研究中,特别是定量蛋白质组研究中备受关注.本文总结了影响肽段可检测性的重要因素,分析了已经提出的计算预测方法,并对其在实验研究中的应用进行了综述.
생물질보시단백질조연구중적핵심기술지일,가이실현대규모、고통량적단백질정성화정량분석.유우양품화실험과정자신적복잡성,질보실험적중복성환존재일사문제,태단감정화정량결과유흔대적수궤성,태단적질보검측개솔문제재단백질조연구중,특별시정량단백질조연구중비수관주.본문총결료영향태단가검측성적중요인소,분석료이경제출적계산예측방법,병대기재실험연구중적응용진행료종술.
As the complexity of samples and experimental processes, the repeatability of mass spectrometry experiments is still not satisfactory, the results of peptide identification and quantification show high randomicity), the probability of peptide being detected by mass spectrometry in proteome research, especially in quantitative proteomic study, has received much attention. Therefore, a lot of experimental researches have been done, as well as a number of computational prediction methods have been developed. In this article, we summarized the important factors impacting the peptide detectability, investigated the existing prediction methods) and reviewed their applications in experimental study.