CAJ | 학술논문

目的研究毒蕈环肽a-amanitin对人肝癌细胞SMMC-7721的体外毒性作用.方法运用MTT法研究α-amanitin不同浓度和不同作用时间对体外传代培养的人肝癌细胞SMMC-7721的抑制增殖作用,再进行环肽α-amanitin对细胞毒性作用的数学模型拟合. 结果研究发现,α-amanitin的使用剂量与细胞存活率存在负相关.7.12×10~(-4) mmol/L 和7.12×10~(-10) mmol/L α-amanitin对SMMC-7721作用24 h后,细胞存活率分别为71.25%和87.47%,作用效果分别与10 mmol/L和2.5 mmol/L 环磷酰胺相似.SMMC-7721细胞存活率平方根的反正弦值与α-amanitin浓度的对数变换值的相关和回归分析结果显示二者显著性相关,曲线拟合结果显示三次曲线模型拟合最好,回归方程为Y=31.257 7-12.902X-1.5117X~2-0.0616X~3.对SMMC-7721细胞存活率平方根的反正弦值与α-amanitin作用时间进行相关和回归分析,二者显著性相关,三次曲线模型拟合最好,对应回归方程为Y=89.668 5-8.616 6X+1.791X~2-0.1271X~3.结论环肽a-amanitin对人肝癌细胞SMMC-7721体外增殖有显著抑制作用,且单位摩尔数的α-amanitin抑制作用远强于单位摩尔数的环磷酰胺.
목적 연구독심배태a-amanitin대인간암세포SMMC-7721적체외독성작용.방법 운용MTT법연구α-amanitin불동농도화불동작용시간대체외전대배양적인간암세포SMMC-7721적억제증식작용,재진행배태α-amanitin대세포독성작용적수학모형의합. 결과연구발현,α-amanitin적사용제량여세포존활솔존재부상관.7.12×10~(-4) mmol/L 화7.12×10~(-10) mmol/L α-amanitin대SMMC-7721작용24 h후,세포존활솔분별위71.25%화87.47%,작용효과분별여10 mmol/L화2.5 mmol/L 배린선알상사.SMMC-7721세포존활솔평방근적반정현치여α-amanitin농도적대수변환치적상관화회귀분석결과현시이자현저성상관,곡선의합결과현시삼차곡선모형의합최호,회귀방정위Y=31.257 7-12.902X-1.5117X~2-0.0616X~3.대SMMC-7721세포존활솔평방근적반정현치여α-amanitin작용시간진행상관화회귀분석,이자현저성상관,삼차곡선모형의합최호,대응회귀방정위Y=89.668 5-8.616 6X+1.791X~2-0.1271X~3.결론 배태a-amanitin대인간암세포SMMC-7721체외증식유현저억제작용,차단위마이수적α-amanitin억제작용원강우단위마이수적배린선알.
Objective To study the inhibitory effect of cyclopeptide α-amanitin on human hepatoma SMMC-7721 cells. Methods The SMMC-7721 cells were treated with different concentration and duration of α-amanitin. The cell survival rate (CRS) of SMMC-7721 cells was examined by MTT assay in vitro, and the relative results were fitted with mathematical model. Results The cell survival rate was reversely correlated with the concentration of α-amanitin. The cell growth was restrained strikingly when the cells were treated by 7.12×10~(-10) mmol/L α-amanitin for 24 h. The cell survival rate were 71.25% and 87.47% after treating with 7.12×10~(-4) mmol/L and 7.12×10～10 mmol/L α-amanitin for 24 h, respectively. The arcsine transformation of the CSR square root and the logarithmic transformation of α-amanitin concentration were correlated and regressed. The curve fitting showed that the cubic model was the best one. The regression equation was Y=31.257 7-12.902X-1.511 7X~2-0.061 6X~3. The arcsine transformation of the CSR square root and the action time of α-amanitin were correlated and regressed. The correlation was also significant and the cubic model was the best one. The cubic regression equation was Y=89.668 5-8.6166X+1.791X~2-0.1271X~3. Conclusion The cyclopeptide α-amanitin has significant inhibitory effect on human hepatoma SMMC-7721 cells, and its toxicity is much stronger than cyclophosphamide.