CAJ | 학술논문

目的研究针刺雌性大鼠身体各部位不同穴位对下丘脑促性腺激素释放激素(GnRH)相关神经元活动的影响,以比较身体各部位不同穴位对这类神经元活动的有效性,为针刺治疗生殖内分泌系统疾病提供实验依据.方法用玻璃微电极细胞外记录下丘脑内视前内侧区、弓状核及室旁核区域的GnRH相关神经元放电,以该神经元放电为指标,探察针刺身体不同部位的15个穴位对神经元的激活效应及激活强度的差异.结果针刺15个穴位前后兴奋性神经元放电变化百分率从大到小的排列顺序为:子宫、关元、带脉、三阴交、耳甲、足三里、下关、关元俞、肾俞、中脘、曲池、合谷、内关、肝俞、膻中;从针刺部位来看有明显变化的是:下腹部、后肢、下背部.针刺对抑制性神经元的影响同兴奋性神经元的结果基本一致.结论针刺能有效调节下丘脑-垂体-性腺内分泌轴的活性,但不同穴位的调节作用不同.穴位的调节作用与其所处部位的神经节段支配密切相关,与生殖器官同节段支配的穴位其激活下丘脑中GnRH相关神经元的作用最强.
목적 연구침자자성대서신체각부위불동혈위대하구뇌촉성선격소석방격소(GnRH)상관신경원활동적영향,이비교신체각부위불동혈위대저류신경원활동적유효성,위침자치료생식내분비계통질병제공실험의거.방법 용파리미전겁세포외기록하구뇌내시전내측구、궁상핵급실방핵구역적GnRH상관신경원방전,이해신경원방전위지표,탐찰침자신체불동부위적15개혈위대신경원적격활효응급격활강도적차이.결과 침자15개혈위전후흥강성신경원방전변화백분솔종대도소적배렬순서위:자궁、관원、대맥、삼음교、이갑、족삼리、하관、관원유、신유、중완、곡지、합곡、내관、간유、단중;종침자부위래간유명현변화적시:하복부、후지、하배부.침자대억제성신경원적영향동흥강성신경원적결과기본일치.결론 침자능유효조절하구뇌-수체-성선내분비축적활성,단불동혈위적조절작용불동.혈위적조절작용여기소처부위적신경절단지배밀절상관,여생식기관동절단지배적혈위기격활하구뇌중GnRH상관신경원적작용최강.
Objective To study the influences of acupuncture in different acupoints on activities of hypothalamus GnRH-related neurons in female rats.compare the validity of different acupoints in body's different parts to the neuron activities,and provide experimental basis for the treatment of the diseases of propagative and incretory system with acupuncture.Methods The discharge of GnRH-related neurons in the medial preoptic area,arcuate nucleus and paraventricular nucleus of hypothalamus were recorded extracellularly by using glass microelectrode to study the difference of 15 acupoints in body's different parts in activation and activation intensity to neurons.Results The percentage of activated neuron discharge changes before and after acupuncture in 15 acupoints showed a descending order as follows:Zigong(EXCA1),Guanyuan(CV4),Daimai(GB26),Sanyinjiao(SP6),Erjia,Zusanli(ST36),Xiaguan(ST7),Guanyuanshu(BL26),Shenshu(BL23),Zhongwan(CV12),Quchi(LI11),Hegu(L14),Neiguan (PC6),Ganshu(BL18)and Danzhong(CV17).The acupuncture positions with obvious changes included low abdominal,hind limbs and lower back.The influence of acupuncture on inhibited neurons was as the same as that on activated ones.Conclusion Acupuncture can regulate effectively the activity of hypothalamus-hypophysis-gonadal endocrine axis,but the effect is different in different acupoints.The regulation effect of acupuncture in an acupoint is related closely to the spinal segment dominated position of the same acupoint.The acupoints with the same spinal segment domination of the genital organs have the largest activation on hypothalamus GnRH-related neurons.