CAJ | 학술논문

背景:MC3R基因突变是引起体质量增加的重要因素之一.目的:分析比格犬MC3R基因突变与体质量性状的关系.方法:抽取112只比格犬血液,记录体质量数据,然后提取DNA;采用AS-PCR技术对MC3R基因A167T侠点进行分析,并克隆测序.观察比格犬MC3R基因T167A的基因型分布及其与体质量的关系.结果与结论:167位点表现为A、T2个等位基因和AA、AT及TT 3种基因型;T167A碱基置换对犬体质量有显著影响(P<0.05).表明MC3R基因突变可致犬体质量增加,可作为犬体质量标记的候选基因.
배경:MC3R기인돌변시인기체질량증가적중요인소지일.목적:분석비격견MC3R기인돌변여체질량성상적관계.방법:추취112지비격견혈액,기록체질량수거,연후제취DNA;채용AS-PCR기술대MC3R기인A167T협점진행분석,병극륭측서.관찰비격견MC3R기인T167A적기인형분포급기여체질량적관계.결과여결론:167위점표현위A、T2개등위기인화AA、AT급TT 3충기인형;T167A감기치환대견체질량유현저영향(P<0.05).표명MC3R기인돌변가치견체질량증가,가작위견체질량표기적후선기인.
BACKGROUND:Melanocortin-3 receptor(MC3R)gene mutation is an important factor in weight gain.CIBJECTIVE:To analyze the relationship between MC3R gene mutations and body weight traits in Beagle dogs.METHODS:The blood samples were taken from 112 Beagle dogs,and their body weights were recorded,then their genomic DNA was extracted.The A167T site of MC3R gene was detected by the methods of AS-PCR,and mutant-lype MC3R gene was sequenced.The relationships between MC3R genotypes distribution and body weight were observed.RESULTS AND CONCLUSlON:The A167T site of Beagle canine MC3R gene was found with two alleles A and T and three genotypes AA.AT and TT.Statistical analysis showed that the base substitution of T167A had significant influence on canine body weight (P<0.05).It suggested that MC3R gene mutation can lead to a significant increase in canine body weight,which can be used as a candidate gene for body weight labeling.