CAJ | 학술논문

目的采用组织培养技术在体外培养取自人体的睾丸卵黄囊瘤组织,探讨小儿睾丸卵黄囊瘤组织的体外培养方法及生物学特性.方法采用组织块培养法培养1例睾丸卵黄囊瘤患儿手术的标本,从形态学、细胞生长动力学、肿瘤内分泌、染色体分析、细胞DNA分析等方面初步研究了小儿睾丸卵黄囊瘤细胞的生物学特性.结果小儿睾丸卵黄囊瘤细胞无论形态学观察,还是功能学测定,均符合卵黄囊瘤细胞的特征,染色体众数39～97条,并具有体外分泌AFP功能.其特有表现为:细胞贴壁生长,呈短梭形或多角形,细胞密集时可多层重叠生长,肿瘤细胞表面有明显的微绒毛,细胞核形态怪异,细胞倍增时间较长.结论小儿睾丸卵黄囊瘤细胞在体外生长稳定,增殖活跃,肿瘤细胞的纯度较高,可以用于后续的实验研究.
목적 채용조직배양기술재체외배양취자인체적고환란황낭류조직,탐토소인고환란황낭류조직적체외배양방법급생물학특성.방법 채용조직괴배양법배양1례고환란황낭류환인수술적표본,종형태학、세포생장동역학、종류내분비、염색체분석、세포DNA분석등방면초보연구료소인고환란황낭류세포적생물학특성.결과 소인고환란황낭류세포무론형태학관찰,환시공능학측정,균부합란황낭류세포적특정,염색체음수39～97조,병구유체외분비AFP공능.기특유표현위:세포첩벽생장,정단사형혹다각형,세포밀집시가다층중첩생장,종류세포표면유명현적미융모,세포핵형태괴이,세포배증시간교장.결론 소인고환란황낭류세포재체외생장은정,증식활약,종류세포적순도교고,가이용우후속적실험연구.
Objective To establish a method for the culture of testicular yolk sac tumor in childhood and then investigate biological characteristics in vitro. Methods One specimen from testicular yolk sac tumor was cultured in vitro. Testicular yolk sac tumor cell lines were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation. Results Morphological observation and functional analysis show that cell lines have characteristics of testicular yolk sac tumor. The number of chromosomes varied from 39 to 97. Turours were immunostained positively for AFP in vitro and found to form multiple layers with microvilli. The nuclei were variable and bizarre in size and shape. Conclusions Testicular yolk sac tumor cell lines were cultured proliferated in a stable manner in vitro, which provides a convenient and economical object for basic researches on yolk sac tumor in future.