中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
7期
868-870
,共3页
贺红焰%沈宁%刘建%史小玲%王明勇
賀紅燄%瀋寧%劉建%史小玲%王明勇
하홍염%침저%류건%사소령%왕명용
血管紧张素类%肾小球%细胞增殖%原癌基因蛋白质c-fos
血管緊張素類%腎小毬%細胞增殖%原癌基因蛋白質c-fos
혈관긴장소류%신소구%세포증식%원암기인단백질c-fos
Angiotensins%Kidney glomerulus%Cell proliferation%Proto-oncogene protein c-fos
目的 观察血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素-Ⅱ(Ang-II)诱导的肾小球系膜细胞(GMC)增殖过程中c-fos表达的影响.方法 体外培养GMC,Ang-II为刺激因子,不同浓度Ang-(1-7)为干预因子.用结晶紫计数法检测GMC数目的 变化;Western blot检测GMC中c-fos蛋白表达的变化.结果 Ang-(1-7)呈剂量依赖性地抑制Ang-II诱导的GMC增殖和GMC内c-fos蛋白表达.结论 Ang-(1-7)可能通过抑制c-fos的表达,抑制Ang-II诱导的GMC增殖.
目的 觀察血管緊張素-(1-7)[Ang-(1-7)]對血管緊張素-Ⅱ(Ang-II)誘導的腎小毬繫膜細胞(GMC)增殖過程中c-fos錶達的影響.方法 體外培養GMC,Ang-II為刺激因子,不同濃度Ang-(1-7)為榦預因子.用結晶紫計數法檢測GMC數目的 變化;Western blot檢測GMC中c-fos蛋白錶達的變化.結果 Ang-(1-7)呈劑量依賴性地抑製Ang-II誘導的GMC增殖和GMC內c-fos蛋白錶達.結論 Ang-(1-7)可能通過抑製c-fos的錶達,抑製Ang-II誘導的GMC增殖.
목적 관찰혈관긴장소-(1-7)[Ang-(1-7)]대혈관긴장소-Ⅱ(Ang-II)유도적신소구계막세포(GMC)증식과정중c-fos표체적영향.방법 체외배양GMC,Ang-II위자격인자,불동농도Ang-(1-7)위간예인자.용결정자계수법검측GMC수목적 변화;Western blot검측GMC중c-fos단백표체적변화.결과 Ang-(1-7)정제량의뢰성지억제Ang-II유도적GMC증식화GMC내c-fos단백표체.결론 Ang-(1-7)가능통과억제c-fos적표체,억제Ang-II유도적GMC증식.
Objective To investigate the effect of angiotensin-(1-7) on the expression of cellular c-fos in angiotensin II -induced proliferative glomerular mesangial cells (GMC). Methods GMC were treated with angiotensin II and different dose of angiotensin-(1-7). GMC number were evaluated by crystal violet staining and the expression of c-foe were detected by western blot. Results Angiotensin-(1-7) inhibit angiotensin II -induced GMC proliferation as well as the expression c-foe in a concentration dependent manner. Conclusion c-fos is involved in the inhibiting effects of angiotensin-(1-7) on angiotensin II -induced GMC proliferation.
