中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2008年
9期
909-914
,共6页
黄支密%糜祖煌%储秋菊%单浩%熊春林%邹玉秀%夏守慧%秦玲
黃支密%糜祖煌%儲鞦菊%單浩%熊春林%鄒玉秀%夏守慧%秦玲
황지밀%미조황%저추국%단호%웅춘림%추옥수%하수혜%진령
肺炎克雷伯菌%16S rRNA甲基化酶%基因
肺炎剋雷伯菌%16S rRNA甲基化酶%基因
폐염극뢰백균%16S rRNA갑기화매%기인
Klebsiella pneumoniae%16S rRNA methylase%Gene
目的 了解临床分离肺炎克雷伯菌中16S rRNA甲基化酶基因、氨基糖苷类修饰酶(AMEs)基因存在状况及其遗传学背景.方法 在2005年9月至2006年4月间从住院患者中分离25株肺炎克雷伯菌,采用聚合酶链反应及序列分析的方法分析6种16S rRNA甲基化酶基因(armA、rmtA、rmtB、rmtC、rmtD和npmA)、6种AMEs基因[aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰ、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ]、3类整合子(Ⅰ、Ⅱ、Ⅲ类)遗传标记整合酶基因(intI1、intI2、intI3)、汞离子还原酶基因merA(为转座子Tn21和Tn501共同的遗传标记)、tnpA基凶(为转座子Tn1、Tn2、Tn3和Tn1000共同的遗传标记).结果 25株中,有5种基因rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ、ant(3")-Ⅰ、intI1阳性,阳性株数(%)分别为15株(60.0%)、1株(4.0%)、12株(48.0%)、15株(60.0%)、24株(96.0%),其余12种基因均阴性;6种AMEs基因总阳性率为84.0%(21/25).结论临床分离的肺炎克雷伯菌中rmtB基因和AMEs基因阳性率均较高,在肺炎克雷伯菌中检出rmtB基因为中国大陆地区首次报道.
目的 瞭解臨床分離肺炎剋雷伯菌中16S rRNA甲基化酶基因、氨基糖苷類脩飾酶(AMEs)基因存在狀況及其遺傳學揹景.方法 在2005年9月至2006年4月間從住院患者中分離25株肺炎剋雷伯菌,採用聚閤酶鏈反應及序列分析的方法分析6種16S rRNA甲基化酶基因(armA、rmtA、rmtB、rmtC、rmtD和npmA)、6種AMEs基因[aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰ、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ]、3類整閤子(Ⅰ、Ⅱ、Ⅲ類)遺傳標記整閤酶基因(intI1、intI2、intI3)、汞離子還原酶基因merA(為轉座子Tn21和Tn501共同的遺傳標記)、tnpA基兇(為轉座子Tn1、Tn2、Tn3和Tn1000共同的遺傳標記).結果 25株中,有5種基因rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ、ant(3")-Ⅰ、intI1暘性,暘性株數(%)分彆為15株(60.0%)、1株(4.0%)、12株(48.0%)、15株(60.0%)、24株(96.0%),其餘12種基因均陰性;6種AMEs基因總暘性率為84.0%(21/25).結論臨床分離的肺炎剋雷伯菌中rmtB基因和AMEs基因暘性率均較高,在肺炎剋雷伯菌中檢齣rmtB基因為中國大陸地區首次報道.
목적 료해림상분리폐염극뢰백균중16S rRNA갑기화매기인、안기당감류수식매(AMEs)기인존재상황급기유전학배경.방법 재2005년9월지2006년4월간종주원환자중분리25주폐염극뢰백균,채용취합매련반응급서렬분석적방법분석6충16S rRNA갑기화매기인(armA、rmtA、rmtB、rmtC、rmtD화npmA)、6충AMEs기인[aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰ、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ]、3류정합자(Ⅰ、Ⅱ、Ⅲ류)유전표기정합매기인(intI1、intI2、intI3)、홍리자환원매기인merA(위전좌자Tn21화Tn501공동적유전표기)、tnpA기흉(위전좌자Tn1、Tn2、Tn3화Tn1000공동적유전표기).결과 25주중,유5충기인rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ、ant(3")-Ⅰ、intI1양성,양성주수(%)분별위15주(60.0%)、1주(4.0%)、12주(48.0%)、15주(60.0%)、24주(96.0%),기여12충기인균음성;6충AMEs기인총양성솔위84.0%(21/25).결론림상분리적폐염극뢰백균중rmtB기인화AMEs기인양성솔균교고,재폐염극뢰백균중검출rmtB기인위중국대륙지구수차보도.
Objective To investigate the presence and genetic background of 16S rRNA methylase gene and Aminoglycoside modifying enzymes(AMEs) genes in Klebsiella pneumoniae isolated from the People's Liberation Army 98th HospitaI,Huzhou district,Zhejiang province,China.Methods 25 strains of Klebsiella pneumoniae were isolated from the inpatienta between September,2005 and April,2006.6 kinds of 16S rRNA methylase gene (including armA,rmtA,rmtB,rmtC,rmtD and npmA ),6 kinds of AMEs genes [ including aac (3)-Ⅰ,sac (3)-Ⅱ,sac (6')-Ⅰ,aac (6')-Ⅱ,ant (3")-Ⅰand ant(2")-Ⅰ],intI1,intI2,intI3,mercuric reductase gene merA (merA gene were the collective genetic markers of transposona of Tn21 and Tn501 ) and tnpA ( tnpA gene were the collective genetic markers of transposons of Tn1,Tn2,Tn3 and Tn1000) were analyzed by PCR and verificated by DNA sequencing.Results In 25 strains of Klebsiella pneumoniae,the positive rate of genes of rmtB,sac (3)-Ⅱ,sac (6')-Ⅰ,ant(3")-Ⅰ and intI1 were 60.0%(15/25),4.0%(1/25),48.0%(12/25),60.0%(15/25) and 96.0%(24/25),respectively.The rest 12 kinds of genes were all tested negative.The total positive rate of 6 kinds of AMEs gene was 84.0%(21/25).Conclusion There were very high positive rate on both genes of rmtB and AMEs genotypes in Klebsiella pneumoniae isolated from inpatients,and this was the first report of the emergence of 16S rRNA methylase gene rmtB in Klebsiella pneumoniae identified in Zhejiang province,China.
