CAJ | 학술논문

目的通过对化学因素诱发的4种多发性抽动症(TS)模型效度的比较,为TS动物模型的选择应用提供理论和实验依据.方法采用不同化学造模剂建立4种TS模型-AMP模型、APO模型、IDPN模型、DOI模型,通过自主活动次数、爬杆时间、脑纹状体单胺类递质检测,分别从模型的表面效度、预测效度、结构效度方面比较评价4种TS动物模型.结果 AMP模型组与空白组比较,自主活动次数增加(t=4.746,P=0.000),脑纹状体DOPAC含量升高[(0.99±0.18)ng/mg,P=0.029],NE含量下降[(0.11±0.033)ng/mg,P=0.012];APO模型组与空白组比较,爬杆时间延长(t=3.353,P=0.004),脑纹状体DA、5-HT含量升高[分别为(10.19±1.23)ng/mg,(0.54±0.08)ng/mg,P=0.019,P=0.002],DOPAC、HVA含量降低[分别为(0.63±0.11)ng/mg,(0.45±0.04)ng/mg,均P＜0.01];DOI模型组与空白组比较,脑纹状体DA、DOPAC、HVA含量下降[分别为(13.66±1.55)ng/mg,(0.80±0.11)ng/mg,(1.04±0.14)ng/mg,P=0.029,P=0.001,P=0.004];IDPN300组与空白组比较,脑纹状体5-HT含量升高[(0.77±0.09)ng/mg,P=0.031].结论 AMP模型表面效度短暂,IDPN模型表面效度稳定、持久,AMP模型、APO模型、DOI模型具有预测效度,AMP模型、APO模型、DOI模型、IDPN模型具有成为结构效度模型的潜能.
목적 통과대화학인소유발적4충다발성추동증(TS)모형효도적비교,위TS동물모형적선택응용제공이론화실험의거.방법 채용불동화학조모제건립4충TS모형-AMP모형、APO모형、IDPN모형、DOI모형,통과자주활동차수、파간시간、뇌문상체단알류체질검측,분별종모형적표면효도、예측효도、결구효도방면비교평개4충TS동물모형.결과 AMP모형조여공백조비교,자주활동차수증가(t=4.746,P=0.000),뇌문상체DOPAC함량승고[(0.99±0.18)ng/mg,P=0.029],NE함량하강[(0.11±0.033)ng/mg,P=0.012];APO모형조여공백조비교,파간시간연장(t=3.353,P=0.004),뇌문상체DA、5-HT함량승고[분별위(10.19±1.23)ng/mg,(0.54±0.08)ng/mg,P=0.019,P=0.002],DOPAC、HVA함량강저[분별위(0.63±0.11)ng/mg,(0.45±0.04)ng/mg,균P＜0.01];DOI모형조여공백조비교,뇌문상체DA、DOPAC、HVA함량하강[분별위(13.66±1.55)ng/mg,(0.80±0.11)ng/mg,(1.04±0.14)ng/mg,P=0.029,P=0.001,P=0.004];IDPN300조여공백조비교,뇌문상체5-HT함량승고[(0.77±0.09)ng/mg,P=0.031].결론 AMP모형표면효도단잠,IDPN모형표면효도은정、지구,AMP모형、APO모형、DOI모형구유예측효도,AMP모형、APO모형、DOI모형、IDPN모형구유성위결구효도모형적잠능.
Objective To provide theoretical and experimental proof for selecting and implying Tourette's syndrome(TS) animal models, validities of four TS models induced by chemical factors were compared. Methods Four TS models,namely AMP model,APO model,DO1 model and IDPN model were built up by using different chemical modeling agents. Through detecting spontaneous movement, climbing time and monoamine transmitters levels in striatum, four TS animal models were compared and evaluated from three levels of validities-face, prediction,construct. Results Compared with control group, spontaneous movement times raised ( t = 4. 746, P =0. 000) and level of DOPAC ( (0.99 ± 0. 177 ) ng/mg) in striatum increased (P = 0.029 ), and level of NE in striatum decreased in AMP model group( (0.11 ± 0.033 )ng/mg, P = 0.012). Compared with control group, climbing time prolonged (P = 0. 004) and levels of DA ( ( 10. 19 ± 1.23 ) ng/mg), 5-HT ( ( 0. 54 ± 0.08 ) ng/mg) in striatum raised(P=0. 019, P=0. 002),at the same time ,levels of DOPAC( (0.63 ±0.11 )ng/mg),HVA ((0.45 ±0.04 ) ng/mg) in striatum reduced (P ＜ 0.01 ) in APO model group; Compared with control group, levels of DA ( ( 13.66 ± 1.55 ) ng/mg), DOPAC( (0.80 ±0. 11 ) ng/mg), HVA( ( 1.04 ± 0.14) ng/mg) grew downwards in striatum of DOI model mice(P=0.029,P=0.001, P= 0.004). Compared with control group, level of 5-HT in striatum increased in IDPN300 group ( (0.77 ± 0.09) ng/mg, P = 0.031 ). Conclusion Face validity of AMP model is temporal and that of IDPN model is steady and persistent. AMP model,APO model and DOI model possess predictive validity. AMP model,APO model,DOI model and IDPN model have potentiality of becoming construct validity model.